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Low-level laser therapy modulates cyclo-oxygenase-2 expression during bone repair in rats

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Abstract

The goal of this study was to analyze the role of cyclo-oxygenase-2 following bone repair in rats submitted to low-level laser therapy. A total of 48 rats underwent surgery to inflict bone defects in their tibias having been randomly distributed into two groups: negative control and laser exposed group, i.e., the animals were treated with low-level laser therapy by means of gallium arsenide laser at 16 J/cm2. The animals were killed after 48 h, 7 days, 14 days, or 21 days. The tibias were removed for morphological, morphometric, and immunohistochemistry analysis for cyclo-oxygenase-2. Statistical significant differences (P < 0.05) were observed in the quality of bone repair and quantity of formed bone between groups 14 days after surgery in the laser exposed group. In the same way, cyclo-oxygenase-2 immunoreactivity was more intense in bone cells for intermediate periods evaluated in this group. Taken together, such results suggest that low-level laser therapy is able to improve bone repair in the tibia of rats after 14 days of surgery as a result of an up-regulation for cyclo-oxygenase-2 expression in bone cells.

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Acknowledgments

The authors are grateful to Maira Cristina Rondina Couto for histology assistance and Wilson Aparecido Orcini for morphometry assistance. R V Ferino is a recipient of a Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) fellowship [Programa Institucional de Bolsas de Iniciação Científica (PIBIC)].

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Authors and Affiliations

  1. Department of Oral Maxillofacial Surgery, School of Dentistry, University of the Sacred Heart (USC), Bauru, SP, Brazil

    Mariza A. Matsumoto, Rafael V. Ferino & Giovanni F. Monteleone

  2. Department of Biosciences, Federal University of Sao Paulo (UNIFESP), Av. Ana Costa 95, Santos, SP, 11060-001, Brazil

    Daniel A. Ribeiro

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  1. Mariza A. Matsumoto
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  2. Rafael V. Ferino
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Correspondence to Daniel A. Ribeiro.

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Matsumoto, M.A., Ferino, R.V., Monteleone, G.F. et al. Low-level laser therapy modulates cyclo-oxygenase-2 expression during bone repair in rats. Lasers Med Sci 24, 195–201 (2009). https://doi.org/10.1007/s10103-008-0544-4

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  • DOI: https://doi.org/10.1007/s10103-008-0544-4

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