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Low-level laser therapy can reduce lipopolysaccharide-induced contractile force dysfunction and TNF-α levels in rat diaphragm muscle

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Abstract

Our objective was to investigate if low-level laser therapy (LLLT) could improve respiratory function and inhibit tumor necrosis factor (TNF-α) release into the diaphragm muscle of rats after an intravenous injection of lipopolysaccharide (LPS) (5 mg/kg). We randomly divided Wistar rats in a control group without LPS injection, and LPS groups receiving either (a) no therapy, (b) four sessions in 24 h with diode Ga–AsI–Al laser of 650 nm and a total dose of 5.2 J/cm2, or (c) an intravenous injection (1.25 mg/kg) of the TNF-α inhibitor chlorpromazine (CPZ). LPS injection reduced maximal force by electrical stimulation of diaphragm muscle from 24.15 ± 0.87 N in controls, but the addition of LLLT partly inhibited this reduction (LPS only: 15.01 ± 1.1 N vs LPS + LLLT: 18.84 ± 0.73 N, P < 0.05). In addition, this dose of LLLT and CPZ significantly (P < 0.05 and P < 0.01, respectively) reduced TNF-α concentrations in diaphragm muscle when compared to the untreated control group.

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Acknowledgements

This work was funded by the Norwegian Research Council, and FABESP grant no. 05/02117-6.

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Authors and Affiliations

  1. Research Group of Animal Experimental, IP & D UNIVAP R. Shishima Hifumi, 2911, 12240-000, São José dos Campos, SP, Brazil

    F. Aimbire & R. Albertini

  2. Laboratory of Pharmacology and Phototherapy of Inflammation, Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, Butantan, 1524, 05508-900, São Paulo, SP, Brazil

    R. A. B. Lopes-Martins & P. S. L. M. Leonardo

  3. Department of Physiology and Pharmacodynamics, IOC FIOCRUZ, P.O. Box 926, 21045-900, Rio de Janeiro, RJ, Brazil

    H. C. Castro-Faria-Neto

  4. Department of Laser in Biomedicine, Heart Institute, São Paulo, SP, Brazil

    M. C. Chavantes

  5. Research Group of Florescence IP & D, UNIVAP R. Shishima Hifumi, 2911, 12240-000, São José dos Campos, SP, Brazil

    M. T. T. Pacheco

  6. Section of Physiology, Department of Biomedicine, University of Bergen, Haukeland University Hospital, 5020, Bergen, Norway

    V. V. Iversen

  7. Section of Physiotherapy Science, Institute of Public Health and Primary Health Care, University of Bergen, Kalfarveien 31, 5018, Bergen, Norway

    J. M. Bjordal

  8. Bergen University College, Physiotherapy Department, Moellendalsvn. 6, 5009, Bergen, Norway

    J. M. Bjordal

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  1. F. Aimbire
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Aimbire, F., Lopes-Martins, R.A.B., Castro-Faria-Neto, H.C. et al. Low-level laser therapy can reduce lipopolysaccharide-induced contractile force dysfunction and TNF-α levels in rat diaphragm muscle. Lasers Med Sci 21, 238–244 (2006). https://doi.org/10.1007/s10103-006-0405-y

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