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Observations on pulpal response to carbon dioxide laser drilling of dentine in healthy human third molars

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Abstract

Preservation of pulpal health is the primary prerequisite for successful application of laser systems in the hard tissue management of vital teeth. The purpose of this study was to investigate the short and long-term pulpal effects to cavity preparations in healthy human teeth using carbon dioxide (CO2) laser. A total of seven, healthy, third molars that were scheduled to be removed due to space problems were used. After the laser drilling, the occlusal cavities were closed temporarily, and the teeth were extracted 7 days (n=5) and 3 months (n=2) after the operation. The specimens were fixed, decalcified, subdivided and processed for light and transmission electron microscopy. Seven days postoperatively all the five teeth that had been irradiated with the CO2 laser did not reveal any pathological changes in the pulpo-dentine complex. Three months postoperatively the two teeth that were prepared with the laser showed subtle but distinct apposition of tertiary dentine that was lined with intact odontoblasts. One of the specimens at 3 months revealed the presence of a mild, but very circumscribed, pulpal infiltration of chronic inflammatory cells subjacent to the cavity preparation. The latter is unlikely to be due to a direct effect of the laser irradiation but a possible consequence of microleakage of oral antigens and/or other tissue-irritating molecules through the temporary restoration and the remaining dentine thickness (RDT). Although these preliminary histological results suggest that the CO2 laser under investigation induced only minimal response of the dentine-pulp complex when used as a hard-tissue drilling tool, with specific energy settings, pulse duration within thermal relaxation time and emitting radiations at 9.6 μm of wavelength, larger clinical trials involving various types of teeth are necessary to reach definite conclusions for large-scale clinical application of the laser device.

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References

  1. Einstein A (1917) Zur Quantentheorie der Strahlung (On quantem theory of radiation). Physik Z 18:121–128

    Google Scholar 

  2. Schawlow AL, Townes CH (1958) Infrared and optical masers. Phys Rev 112:1940–1949

    Article  Google Scholar 

  3. Maiman TH (1960) Stimulated optical radiation in ruby. Nature 187:493–494

    Google Scholar 

  4. Goldman L, Hornby P, Meyer R, Goldman B (1964) Impact of the laser on dental caries. Nature 203:417

    Google Scholar 

  5. Goldman L, Gray JA, Goldman J, Goldman B, Meyer R (1965) Effect of laser beam impacts on teeth. J Am Dent Assoc 70:601–606

    Google Scholar 

  6. Stern RH, Sognnaes RF (1964) Laser beam effect on dental hard tissues. J Dent Res 43:873

    Google Scholar 

  7. Stern RH, Renger HL, Howell FV (1969) Laser effects on vital dental pulps. Br Dent J 127:26–28

    Google Scholar 

  8. Hoexter DL (2001) Latest advances in laser systems and periodontal surgery. Dent Clin N Am 45:207–212

    Google Scholar 

  9. Kimura Y, Wilder-Smith P, Matsumoto K (2000) Lasers in endodontics: a review. Int Endod J 33:173–185

    Article  Google Scholar 

  10. Sulewski JG (2000) Historical survey of laser dentistry. Dent Clin N Am 44:717–752

    Google Scholar 

  11. Willenborg GC (1988) The future of lasers in clinical dentistry. J Dent Pract Admin 5:141–147

    Google Scholar 

  12. Willenborg GC (1989) Dental laser applications: emerging to maturity. Lasers Surg Med 9:303–313

    Google Scholar 

  13. Jastrow R (1983) How to make nuclear weapons obsolete, 1st edn. Little Brown, Boston, pp 83–88

    Google Scholar 

  14. Anderson RR, Parrish JA (1983) Selective photothermolysis: precise microsurgery by selective absorption of pulsed radiation. Science 220:524–527

    Google Scholar 

  15. Visuri SR, Walsh JT, Wigdor HA (1993) Erbium laser ablation of dental hard tissues: effect of water cooling. Laser Surg Med 18:294–300

    Article  Google Scholar 

  16. Hibst R, Keller U (1989) Experimental studies of the application of Er:YAG laser on dental hard substances: I. Measurement of the ablation rate. Laser Surg Med 9:338–344

    Google Scholar 

  17. Keller U, Hibst R (1989) Experimental studies on the application of the Er:YAG laser on dental hard substances. II Light microscopic and SEM investigations. Laser Surg Med 9:345–351

    Google Scholar 

  18. Walsh JT, Flotte TJ, Deutsch TF (1989) Er:YAG laser ablation of tissue: effect of pulse duration and tissue type on thermal damage. Laser Surg Med 9:314–326

    Google Scholar 

  19. Stanislawki M, Meister J, Mitra T, Ivanenko MM, Zanger K, Hering P (2001) Hard tissue ablation with a free running Er:YAG and Q-switched CO2 laser: a comparative study. Appl Phys 72:115–120

    Google Scholar 

  20. Sekine Y (1995) Histopathological study of Er:YAG laser application to cavity preparation. Jpn J Conserv Dent 38:211–233

    Google Scholar 

  21. Takamori K (2000) A histopathological and immunohistochemical study of dental pulp and pulpal nerve fibers in rats after the cavity preparation using Er:YAG laser. J Endod 26:95–99

    Article  Google Scholar 

  22. Wigdor H, Abt E, Ashrafi S, Walsh JT Jr (1993) The effect of laser on dental hard tissues. J Am Dent Assoc 124:65–70

    Google Scholar 

  23. Cozean C, Arcoria CJ, Pelagalli J, Powell GL (1997) Dentistry for the 21st century. Erbium:YAG laser for teeth. J Am Dent Assoc 128:1080–1087

    Google Scholar 

  24. Nair PNR, Baltensperger MM, Luder HU, Eyrich GKH (2003) Pulpal response to Er:YAG laser drilling of dentine in healthy third molars. Lasers Surg Med 32:203–209

    Article  Google Scholar 

  25. Clayman L, Fuller T, Beckman H (1978) Healing of continuous wave and rapid super-pulsed, carbon dioxide, laser-induced bone defects. J Oral Maxillofac Surg 36:932–937

    Google Scholar 

  26. Miserendino LJ, Neiburger EJ, Walia H, Leubke N, Brantley W (1989) Thermal effects of continuous wave CO2 laser exposure on human teeth: an in vitro study. J Endod 15:302–305

    Google Scholar 

  27. Karnovsky MJ (1965) A formaldehyde glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A–139A

    Google Scholar 

  28. Schroeder HE, Rossinsky K, Müller W (1980) An established routine method for differential staining of epoxy-embedded tissue sections. Microsc Acta 83:111–116

    Google Scholar 

  29. Fraska JM, Parks VR (1965) A routine technique for double staining ultrathin sections using uranyl and lead salts. J Cell Biol 25:157–161

    Article  Google Scholar 

  30. Venable JH, Coggeshall R (1965) A simplified lead citrate stain for use in electron microscopy. J Cell Biol 25:407–408

    Article  CAS  PubMed  Google Scholar 

  31. Melcer J, Chaumette MT, Melcer F (1987) Dental pulp exposed to CO2 laser beam. Lasers Surg Med 7:347–352

    Google Scholar 

  32. Melcer J, Chaumette MT, Melcer F, Zeboulon S, Hasson R, Merard R, Pinaudeau Y, Dejardin J, Weill R (1985) Preliminary report on the effect of the CO2 laser beam on the dentinal pulp of the Macaca mulatta primate and the beagle dog. J Endod 11:1–5

    Google Scholar 

  33. Wigdor HA, Walsh JT Jr (2002) Histologic analysis of the effect on dental pulp of a 9.6-μm CO2 laser. Lasers Surg Med 30:261–266

    Article  Google Scholar 

  34. Zach L, Cohen G (1965) Pulp response to externally applied heat. Oral Surg Oral Med Oral Pathol 19:515–530

    Google Scholar 

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Acknowledgements

We thank Mrs. Ursula Tsuruta and Mrs. Susy Münzel-Pedrazoli for their excellent technical assistance.

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Authors and Affiliations

  1. Institute of Oral Biology, Section of Oral Structures and Development (OSD), Centre of Dental and Oral Medicine, University of Zurich, Plattenstrasse 11, 8028, Zurich, Switzerland

    P. N. R. Nair & H. U. Luder

  2. Cranio-Maxillofacial Surgery, University Hospital, University of Zurich, Zurich, Switzerland

    M. Baltensperger & G. K. H. Eyrich

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  1. P. N. R. Nair
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  2. M. Baltensperger
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  3. H. U. Luder
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  4. G. K. H. Eyrich
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Correspondence to P. N. R. Nair.

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Nair, P.N.R., Baltensperger, M., Luder, H.U. et al. Observations on pulpal response to carbon dioxide laser drilling of dentine in healthy human third molars. Lasers Med Sci 19, 240–247 (2005). https://doi.org/10.1007/s10103-004-0317-7

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  • DOI: https://doi.org/10.1007/s10103-004-0317-7

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