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Biodegradation of azo dyes in industrial effluent: an eco-friendly way toward green technology

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Abstract

Laccase enzyme obtained from Micrococcus luteus was exploited for biodegradation of azo dyes. The enzyme was characterized for optimum growth conditions at pH 7.0, temperature at 37 °C and incubation duration of 72 h. The molecular weight of the enzyme was found to be 63 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Application of enzyme on the two dyes namely C.I. Acid Black 52 and C.I. Acid Blue 113 resulted in biodegradation rate of 92.2 and 94.5 %, respectively. UV–Vis spectra analysis indicated the absence of peaks in the visible region of the experimental samples confirmed the complete biodegradation of dye samples. Further, Fourier transform-infrared spectroscopy results showed the transformation of N=N into N2 or NH3 and then into biomass. Intermediate products of the biodegraded dye sample were analyzed by mass-electrospray ionization spectra. The reduction in BOD and COD values were found to be 93.2, 92.5 and 92.0, 88.7 %, respectively, for the above dyes.

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Acknowledgments

The authors want to thank Council of Scientific and Industrial Research (CSIR) for providing fund and other resources for carrying out the research work successfully.

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Authors and Affiliations

  1. Leather Processing Division, CSIR- Central Leather Research Institute, Adyar, Chennai, 600020, India

    J. Kanagaraj, T. Senthilvelan & R. C. Panda

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  1. J. Kanagaraj
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  2. T. Senthilvelan
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  3. R. C. Panda
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Correspondence to J. Kanagaraj.

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Kanagaraj, J., Senthilvelan, T. & Panda, R.C. Biodegradation of azo dyes in industrial effluent: an eco-friendly way toward green technology. Clean Techn Environ Policy 17, 331–341 (2015). https://doi.org/10.1007/s10098-014-0783-y

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