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Significant shifts in the distribution of vaccine capsular polysaccharide types and rates of antimicrobial resistance of perinatal group B streptococci within the last decade in St. Petersburg, Russia

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Abstract

Evaluation of circulating group B streptococcus (GBS) strains is important to assess the potential effects of GBS prevention strategies in a certain region. This study aimed at estimating the distributions of capsular polysaccharide (CPS) types and pilus profiles, and the rates of antimicrobial resistance among GBS strains isolated from colonized pregnant women and newborns in 2010–2011 and 2017–2018 in St. Petersburg, Russia. A total of 261 GBS isolates have been investigated. Antibiotic susceptibility testing was performed using the disc-diffusion method. CPS types and pilus profiles were determined by using PCR. Over the 9-year period, the resistance of GBS to both erythromycin and clindamycin has significantly increased, exceeding 30% in 2017–2018. The most prevalent CPS types were Ia, III, and V. Significant shifts were observed in the frequency of CPS types III (decreased) and V (increased), which resulted in a significant reduction (from 77 to 63%) in the potential coverage by a trivalent vaccine (including serotypes Ia, Ib, and III), whereas that of a pentavalent vaccine (including serotypes Ia, Ib, II, III, and V) remained largely unchanged (approximately 95%). The most common pilus profiles were PI-1/2a, PI-2a, and PI-1a/2b, and pilus genotype distribution has not changed with time. High and steadily growing resistance of perinatal GBS strains to clindamycin requires restricting its use to penicillin-allergic women at high risk for anaphylaxis and testing the GBS strains for their susceptibility to this antibiotic. A pentavalent CPS-based vaccine covers the vast majority of perinatal GBS strains in Russia.

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Funding

The study was supported by the Ministry of Science and Higher Education of the Russian Federation (contract no. AAAA-A19-119-021290030-0). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Laboratory of Microbiology, D.O. Ott Research Institute of Obstetrics, Gynaecology and Reproductology, 3 Mendeleyevskaya Line, St. Petersburg, 199034, Russia

    Elena Shipitsyna, Kira Shalepo, Svetlana Zatsiorskaya, Anna Krysanova, Maria Razinkova, Alexey Grigoriev & Alevtina Savicheva

  2. Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, St. Petersburg, Russia

    Kira Shalepo, Anna Krysanova & Alevtina Savicheva

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  1. Elena Shipitsyna
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Correspondence to Elena Shipitsyna.

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The study was approved by the Ethical Committee at the D.O. Ott Research Institute of Obstetrics, Gynaecology and Reproductology (approval number 91/2018), and waiver of informed consent was obtained.

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Shipitsyna, E., Shalepo, K., Zatsiorskaya, S. et al. Significant shifts in the distribution of vaccine capsular polysaccharide types and rates of antimicrobial resistance of perinatal group B streptococci within the last decade in St. Petersburg, Russia. Eur J Clin Microbiol Infect Dis 39, 1487–1493 (2020). https://doi.org/10.1007/s10096-020-03864-1

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