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Development and evaluation of MALDI-TOF MS-based serotyping for Streptococcus pneumoniae

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Abstract

Surveillance of Streptococcus pneumoniae serotypes is important for the successful implementation of vaccination strategies to prevent the spread of invasive pneumococcal diseases. The standard method of serotyping of pneumococcal isolates is the phenotypic Neufeld test, which is cost- and labor-intensive. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been implemented as a rapid, simple and inexpensive method for identifying species. We evaluated the performance of MALDI-TOF MS for serotyping ten major serotypes of S. pneumoniae in Japan (serotypes 3, 6B, 15A, 15C, 19A, 19 F, 23A, 24 F, 35B and 38) using the Biotyper and ClinProTools. After optimizing the settings, we validated their serotyping performance for serotypes 3, 15A and 19A using a separate set of isolates that were not used in the creation of the classification algorithms. A total of 574 isolates of S. pneumoniae collected from Japanese nationwide surveillance studies were included. Of these, 407 isolates belonged to the ten major serotypes. Biotyper and ClinProTools correctly identified 77.9 % and 84.0 %, respectively, of the ten major serotype isolates. The validation analysis included a total of 113 isolates of the serotypes 3, 15A and 19A isolates. Biotyper and ClinProTools correctly identified 85.0 % and 69.9 % of the validation cohort isolates, respectively. MALDI-TOF MS has the potential to discriminate the ten major S. pneumoniae serotypes prevalent in Japan.

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Acknowledgments

We are grateful to the members of the Pneumocatch surveillance study group and the Kansai Community Acquired Pneumococcal Pneumonia Study Group for their assistance with the collection of isolates. We are grateful to Michiaki Mishima for his management of these surveillance groups.

Conflict of interest

Yutaka Ito was supported by the research funding (WI 171133) from Pfizer for the surveillance study “Clonarity analysis of Streptococcus pneumoniae isolated from children with pneumococcal infection in Japan”. Takao Fujisawa was supported by an unconditional grant from Pfizer. Bin Chang, Makoto Ohnishi, and Toshiaki Ihara were supported by the Ministry of Health and Labour Sciences Research Grant (H22-shinko-shitei-021) and (H25-shinko-shitei-003).

All other authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan

    S. Nakano, Y. Matsumura, K. Kato, T. Yunoki, G. Hotta, T. Noguchi, M. Yamamoto, M. Nagao, S. Takakura & S. Ichiyama

  2. Department of Respiratory Medicine, Allergy and Rheumatology, Nagoya City University Graduate School of Medical Science, Nagoya, Japan

    Y. Ito

  3. Department of Clinical Research, National Hospital Organization Mie National Hospital, Tsu, Japan

    T. Fujisawa & S. Suga

  4. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan

    B. Chang & M. Ohnishi

  5. Department of Paediatrics, National Hospital Organization Mie National Hospital, Tsu, Japan

    T. Ihara

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  1. S. Nakano
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  2. Y. Matsumura
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  3. Y. Ito
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  4. T. Fujisawa
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  7. K. Kato
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  8. T. Yunoki
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  9. G. Hotta
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  10. T. Noguchi
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  11. M. Yamamoto
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  15. T. Ihara
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  16. S. Ichiyama
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Corresponding author

Correspondence to Y. Matsumura.

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Nakano, S., Matsumura, Y., Ito, Y. et al. Development and evaluation of MALDI-TOF MS-based serotyping for Streptococcus pneumoniae . Eur J Clin Microbiol Infect Dis 34, 2191–2198 (2015). https://doi.org/10.1007/s10096-015-2468-9

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  • DOI: https://doi.org/10.1007/s10096-015-2468-9

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