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Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?

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Abstract

We assessed hepatitis E virus (HEV) seroprevalence in patients with hepatic disorders as well as in human immunodeficiency virus (HIV)-infected patients and emphasised the issue of possible non-specific anti-HEV seroresponse and need for combining diagnostic methods for hepatitis E diagnosis. Over a two-year period, from March 2011 to February 2013, we determined anti-HEV immunoglobulin M (IgM) and IgG by enzyme immunoassays (EIA; Mikrogen, Germany) in 504 hepatitis patients negative for acute viral hepatitis A–C. Furthermore, 88 samples from randomly selected consecutive HIV-infected patients were also analysed. All EIA reactive samples were additionally tested by line immunoblot assays (LIA; Mikrogen, Germany). HEV nested reverse transcription polymerase chain reaction (RT-PCR) was carried out in 14 anti-HEV IgM LIA-positive patients. Anti-HEV IgM or IgG were detected in 16.9 % of patients by EIA and confirmed by LIA in 10.7 % [95 % confidence interval (CI) 8.3–13.7 %] of hepatitis patients. HEV RNA was detected in five patients. The agreement between EIA and LIA assessed by Cohen’s kappa was 0.47 (95 % CI 0.55–0.75) for IgM and 0.83 (95 % CI 0.78–0.93) for IgG. Anti-HEV IgM and IgG seroprevalence in HIV-infected patients was 1.1 %, respectively. Our findings show a rather high HEV seroprevalence in patients with elevated liver enzymes in comparison to HIV-infected patients. Discordant findings by different methods stress the need to combine complementary methods and use a two-tier approach with prudent interpretation of reactive serological results for hepatitis E diagnosis.

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Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. University Hospital for Infectious Diseases, Mirogojska 8, 10000, Zagreb, Croatia

    O. Đaković Rode, R. Mikulić, J. Begovac & A. Vince

  2. Croatian Veterinary Institute, Zagreb, Croatia

    L. Jemeršić & D. Brnić

  3. General Hospital, Slavonski Brod, Croatia

    N. Pandak

  4. School of Medicine, University of Zagreb, Zagreb, Croatia

    J. Begovac & A. Vince

Authors
  1. O. Đaković Rode
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  2. L. Jemeršić
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  3. D. Brnić
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  4. N. Pandak
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  5. R. Mikulić
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  6. J. Begovac
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  7. A. Vince
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Correspondence to O. Đaković Rode.

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Đaković Rode, O., Jemeršić, L., Brnić, D. et al. Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?. Eur J Clin Microbiol Infect Dis 33, 2231–2236 (2014). https://doi.org/10.1007/s10096-014-2187-7

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  • DOI: https://doi.org/10.1007/s10096-014-2187-7

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