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MicroRNA expressions associated with eosinophilic meningitis caused by Angiostrongylus cantonensis infection in a mouse model

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Abstract

Angiostrongylus cantonensis (A. cantonensis) infection is the major cause of eosinophilic meningitis (EM). Severe cases or infant and child cases have poor prognosis. MicroRNAs (miRNAs) play important roles in inflammation; however, little is known about the roles in brain inflammation caused by A. cantonensis. In this study, Illumina deep sequencing and bioinformatics were used to determine the abundance and differential expression of miRNAs in the brain tissues of a mouse model. A total of 648 conserved miRNAs were identified, 157 of which were significantly differentially expressed between infected mice and normal mice. The five most fold-changed miRNAs were miR-511-5p, miR-511-3p, miR-223-3p, miR-155-5p and miR-206-3p. These expressions of miR-511, miR-223, miR-155, miR-206, miR-142 and miR-21a were validated by quantitative reverse transcription polymerase chain reaction (RT-PCR). The analysis of these miRNAs showed that miR-511-3p was more abundant than the miR-511-5p strand, and increased to a peak in 21 days after A. cantonensis infection, miR-223 might be a potential indicator of disease severity and the upregulation of miR-155-5p after stimulation with the somatic antigen of phase IV A. cantonensis implied its involvement in the central nervous system (CNS) inflammation induced by A. cantonensis infection. These observations suggest that miRNAs may play important roles in the regulation of EM caused by A. cantonensis infection.

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Acknowledgments

The authors would like to thank Beijing Berry Genomic Corporation for providing the scientific assistance. This work was supported by a grant from the National Basic Research Program of China (2010CB530004) and the National Natural Science Foundation of China (grant nos. 81271855 and 81261160324).

Conflict of interest

The authors declare that they have no competing interest.

Author contributions

Conceived and designed the experiments: Z.W. and Y.Z. Performed the experiments: L.Y. and X.Z. Analysed the data: Q.L. and L.Y. Contributed reagents/materials/analysis tools: X.Z., H.Z. and X.S. Wrote the paper: L.Y. Reviewed the manuscript: Z.W., Q.L. and Z.L.

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Authors and Affiliations

  1. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China

    L. Yu, X. Zeng, Z. Lv, H. Zheng, X. Sun & Z. Wu

  2. Key Laboratory for Tropical Diseases Control, The Ministry of Education, Sun Yat-sen University, Guangzhou, China

    L. Yu, X. Zeng, Z. Lv, H. Zheng, X. Sun & Z. Wu

  3. Department of Preventive Medicine, School of Medicine, Ningbo University, Ningbo, China

    Q. Liao

  4. Advanced Computing Research Laboratory, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, China

    Y. Zhao

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  1. L. Yu
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  2. Q. Liao
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  3. X. Zeng
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  4. Z. Lv
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  5. H. Zheng
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  7. X. Sun
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Corresponding author

Correspondence to Z. Wu.

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Table S1

Distribution of small RNAs categorisation in the EM model (21d) and controls (Con) (XLS 18 kb)

Table S2

Expression profiles of known miRNAs in the EM model (21d) and control (Con) (XLS 74 kb)

Table S3

The significantly differentially expressed known miRNAs in the EM model (21d) and control (Con) (XLS 50 kb)

Table S4

The enriched KEGG pathways of target genes for differentially expressed miRNAs (XLS 22 kb)

Figure S1

The overall flow of small RNA libraries construction and deep sequencing (JPEG 17 kb)

High resolution (TIFF 84 kb)

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Yu, L., Liao, Q., Zeng, X. et al. MicroRNA expressions associated with eosinophilic meningitis caused by Angiostrongylus cantonensis infection in a mouse model. Eur J Clin Microbiol Infect Dis 33, 1457–1465 (2014). https://doi.org/10.1007/s10096-014-2087-x

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  • DOI: https://doi.org/10.1007/s10096-014-2087-x

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