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Identification of blood and wound isolates of C. canimorsus and C. cynodegmi using VITEK2 and MALDI-TOF

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Abstract

Capnocytophaga canimorsus and C. cynodegmi are gram negative bacteria that can be transmitted to humans from dogs or cats and cause serious infections. Routine bacteriological methods, including fermentation and phenotypic tests are insufficient to correctly identify C. canimorsus or C. cynodegmi. The aim of this study was to evaluate the performance of VITEK2 and MALDI-TOF in identification of these bacteria. Twenty two isolates that were identified as C. canimorsus / C. cynodegmi by 16S rRNA sequencing were included in the study and were further investigated with VITEK2 and MALDI-TOF. A Capnocytophaga species-specific PCR was used as the reference method. Out of 22 included isolates, the species-specific PCR identified six blood isolates as C. canimorsus and 14 wound isolates as C. cynodegmi. Two isolates could not be identified with the reference method. VITEK2 identified 10/20 isolates correctly to Capnocytophaga spp. MALDI-TOF analysis correctly identified 6/6 C. canimorsus and 13/14 C. cynodegmi isolates. The mean time to identification with VITEK2 was 6 hours whereas MALDI-TOF required approximately 10 minutes per sample. Here we show that MALDI-TOF rapidly identified C. canimorsus and C. cynodegmi and thus constitutes a valuable diagnostic tool in the clinical laboratory.

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Acknowledgments

PB holds a Postdoc position from Karolinska Institutet and Stockholm County Council. In addition, he has received grants from the Magnus Bergwall and Åke Wiberg foundations as well as from the Karolinska Institutet and Swedish Society for Medical Microbiology. We extend our gratitude to Mrs Britta Loré, Falun Central Hospital for providing clinical strains and epidemiologic data.

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Nothing to declare.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institutet and Karolinska University Hospital, Huddinge, Stockholm, Sweden

    S. Zangenah, V. Özenci, S. Boräng & P. Bergman

  2. Department of Medicine, Centre for Infectious Medicine (CIM), Karolinska Institutet and Karolinska University Hospital, Huddinge, Stockholm, Sweden

    S. Zangenah & P. Bergman

Authors
  1. S. Zangenah
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  2. V. Özenci
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  3. S. Boräng
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  4. P. Bergman
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Correspondence to P. Bergman.

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Zangenah, S., Özenci, V., Boräng, S. et al. Identification of blood and wound isolates of C. canimorsus and C. cynodegmi using VITEK2 and MALDI-TOF. Eur J Clin Microbiol Infect Dis 31, 2631–2637 (2012). https://doi.org/10.1007/s10096-012-1606-x

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  • DOI: https://doi.org/10.1007/s10096-012-1606-x

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