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Comparison of an in-house method and the commercial Sepsityper™ kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry

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Abstract

The identification of bacteria directly from positive blood cultures using matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is a new challenge to microbiologists. However, the protocols previously described are often difficult to implement in routine and comparisons are not always possible due to the variability of interpretative criteria. This study evaluated the analytical and practical performances of an in-house (IH) method, adapted from previous protocols, and the Sepsityper™ kit (Bruker Daltonics, Bremen, Germany). Positive blood cultures from 63 different patients were prospectively evaluated by both methods. To enhance the sensitivity of these methods, lowered cut-offs were assessed and validated on 66 additional samples. The IH method produced 86.4% and 73.7% correct genus and species identifications, respectively, when using the lowered cut-offs of 1.4 and 1.6 for correct genus and species identifications. The Sepsityper™ kit showed similar results (78.0% and 68.4% correct genus and species identification, respectively). However, the IH method is ten-fold less expensive than the commercial option (0.72 vs. 7.45 €/analysis) and its turnaround time is approximately 20 min versus the nearly 40 min required for the Sepsityper™ kit, which includes an extraction step. Finally, the IH method was introduced twice-daily in our routine practice.

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Acknowledgements

We thank Michèle Dramaix from the School of Public Health, Université Libre de Bruxelles, for her help in the statistical analysis process. The technical assistance provided by David Fage was also greatly appreciated.

This work has been partially presented at the 51st Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), Chicago, IL, USA, September 2011 (Poster D1292).

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Authors and Affiliations

  1. Department of Microbiology, Saint-Pierre University Hospital and Jules Bordet Institute, rue Haute 322, 1000, Brussels, Belgium

    D. Martiny, A. Dediste & O. Vandenberg

  2. Infectious Diseases Epidemiological Unit, School of Public Health, Université Libre de Bruxelles, Brussels, Belgium

    O. Vandenberg

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  1. D. Martiny
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  2. A. Dediste
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  3. O. Vandenberg
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Correspondence to D. Martiny.

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Martiny, D., Dediste, A. & Vandenberg, O. Comparison of an in-house method and the commercial Sepsityper™ kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 31, 2269–2281 (2012). https://doi.org/10.1007/s10096-012-1566-1

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