Skip to main content

Advertisement

SpringerLink
Log in

Restriction modification (RM) tests associated to additional molecular markers for screening prevalent MRSA clones in Brazil

  • Article
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

In this study, we associated the restriction modification (RM) tests to the polymerase chain reaction (PCR) detection of molecular markers (SCCmec III, seh, agr II-SCCmec IV, and lukSF) for revealing the main methicillin-resistant Staphylococcus aureus (MRSA) clones circulating in Brazil. This simple and rapid approach allowed a precise classification of the MRSA analyzed when compared with pulsed-field gel electrophoresis (PFGE) data.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Appelbaum PC (2006) MRSA—the tip of the iceberg. Clin Microbiol Infect 12(Suppl 2):3–10

    Article  PubMed  CAS  Google Scholar 

  2. Deurenberg RH, Stobberingh EE (2008) The evolution of Staphylococcus aureus. Infect Genet Evol 8:747–763

    Article  PubMed  CAS  Google Scholar 

  3. Feng Y, Chen CJ, Su LH, Hu S, Yu J, Chiu CH (2008) Evolution and pathogenesis of Staphylococcus aureus: lessons learned from genotyping and comparative genomics. FEMS Microbiol Rev 32:23–37

    Article  PubMed  CAS  Google Scholar 

  4. Cury GG, Mobilon C, Stehling EG, Lancellotti M, de Ramos CM, Martinez R, Brocchi M, Silveira WD (2009) Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in two metropolitan areas of São Paulo State, southeast Brazil. Braz J Infect Dis 13:165–169

    Article  PubMed  CAS  Google Scholar 

  5. Ribeiro A, Coronado AZ, Silva-Carvalho MC, Ferreira-Carvalho BT, Dias C, Rozenbaum R, Del Peloso PF, da Costa Ferreira Leite C, Teixeira LA, Figueiredo AMS (2007) Detection and characterization of international community-acquired infections by methicillin-resistant Staphylococcus aureus clones in Rio de Janeiro and Porto Alegre cities causing both community- and hospital-associated diseases. Diagn Microbiol Infect Dis 59:339–345

    Article  PubMed  CAS  Google Scholar 

  6. Teixeira LA, Resende CA, Ormonde LR, Rosenbaum R, Figueiredo AMS, de Lencastre H, Tomasz A (1995) Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil. J Clin Microbiol 33:2400–2404

    PubMed  CAS  Google Scholar 

  7. Aires de Sousa M, Sanches IS, Ferro ML, Vaz MJ, Saraiva Z, Tendeiro T, Serra J, de Lencastre H (1998) Intercontinental spread of a multidrug-resistant methicillin-resistant Staphylococcus aureus clone. J Clin Microbiol 36:2590–2596

    PubMed  CAS  Google Scholar 

  8. Sousa-Junior FC, Silva-Carvalho MC, Fernandes MJ, Vieira MF, Pellegrino FL, Figueiredo AMS, de Melo MC, Milan EP (2009) Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil. Braz J Med Biol Res 42:877–881

    Article  PubMed  Google Scholar 

  9. Silva-Carvalho MC, Bonelli RR, Souza RR, Moreira S, dos Santos LCG, de Souza Conceição M, de Mello Junior SJ, Carballido JM, Rito PN, Vieira VV, Teixeira LA, Figueiredo AMS (2009) Emergence of multiresistant variants of the community-acquired methicillin-resistant Staphylococcus aureus lineage ST1-SCCmecIV in 2 hospitals in Rio de Janeiro, Brazil. Diagn Microbiol Infect Dis 65:300–335

    Article  PubMed  Google Scholar 

  10. de Miranda OP, Silva-Carvalho MC, Ribeiro A, Portela F, Cordeiro RP, Caetano N, Vidal CFL, Figueiredo AMS (2007) Emergence in Brazil of methicillin-resistant Staphylococcus aureus isolates carrying SCCmecIV that are related genetically to the USA800 clone. Clin Microbiol Infect 13:1165–1172

    Article  PubMed  Google Scholar 

  11. Melo MCN, Silva-Carvalho MC, Ferreira RL, Coelho LR, Souza RR, Gobbi CN, Rozenbaum R, Solari CA, Ferreira-Carvalho BT, Figueiredo AMS (2004) Detection and molecular characterization of a gentamicin-susceptible, methicillin-resistant Staphylococcus aureus (MRSA) clone in Rio de Janeiro that resembles the New York/Japanese clone. J Hosp Infect 58:276–285

    Article  PubMed  CAS  Google Scholar 

  12. Ribeiro AM, Dias C, Silva-Carvalho MC, Berquó L, Ferreira FA, Santos RN, Ferreira-Carvalho BT, Figueiredo AMS (2005) First report of infection with community-acquired methicillin-resistant Staphylococcus aureus in South America. J Clin Microbiol 43:1985–1988

    Article  PubMed  CAS  Google Scholar 

  13. Scribel LV, Silva-Carvalho MC, Souza RR, Superti SV, Kvitko CH, Figueiredo AMS, Zavascki AP (2009) Clinical and molecular epidemiology of methicillin-resistant Staphylococcus aureus carrying SCCmecIV in a university hospital in Porto Alegre, Brazil. Diagn Microbiol Infect Dis 65:457–461

    Article  PubMed  CAS  Google Scholar 

  14. Enright MC, Day NPJ, Davies CE, Peacock SJ, Spratt BG (2000) Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38:1008–1015

    PubMed  CAS  Google Scholar 

  15. Frénay HM, Bunschoten AE, Schouls LM, van Leeuwen WJ, Vandenbroucke-Grauls CM, Verhoef J, Mooi FR (1996) Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism. Eur J Clin Microbiol Infect Dis 15:60–64

    Article  PubMed  Google Scholar 

  16. Harmsen D, Claus H, Witte W, Rothgänger J, Claus H, Turnwald D, Vogel U (2003) Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 41:5442–5448

    Article  PubMed  CAS  Google Scholar 

  17. Tenover FC, Gay EA, Frye S, Eells SJ, Healy M, McGowan JE Jr (2009) Comparison of typing results obtained for methicillin-resistant Staphylococcus aureus isolates with the DiversiLab system and pulsed-field gel electrophoresis. J Clin Microbiol 47:2452–2457

    Article  PubMed  CAS  Google Scholar 

  18. Cockfield JD, Pathak S, Edgeworth JD, Lindsay JA (2007) Rapid determination of hospital-acquired meticillin-resistant Staphylococcus aureus lineages. J Med Microbiol 56:614–619

    Article  PubMed  CAS  Google Scholar 

  19. Diep BA, Perdreau-Remington F, Sensabaugh GF (2003) Clonal characterization of Staphylococcus aureus by multilocus restriction fragment typing, a rapid screening approach for molecular epidemiology. J Clin Microbiol 41:4559–4564

    Article  PubMed  CAS  Google Scholar 

  20. Hardy KJ, Oppenheim BA, Gossain S, Gao F, Hawkey PM (2006) Use of variations in staphylococcal interspersed repeat units for molecular typing of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 44:271–273

    Article  PubMed  CAS  Google Scholar 

  21. Strandén A, Frei R, Widmer AF (2003) Molecular typing of methicillin-resistant Staphylococcus aureus: can PCR replace pulsed-field gel electrophoresis? J Clin Microbiol 41:3181–3186

    Article  PubMed  Google Scholar 

  22. Waldron DE, Lindsay JA (2006) Sau1: a novel lineage-specific type I restriction-modification system that blocks horizontal gene transfer into Staphylococcus aureus and between S. aureus isolates of different lineages. J Bacteriol 188:5578–5585

    Article  PubMed  CAS  Google Scholar 

  23. Boye K, Bartels MD, Andersen IS, Møller JA, Westh H (2007) A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V. Clin Microbiol Infect 13:725–727

    Article  PubMed  CAS  Google Scholar 

  24. Pacheco AB, Guth BE, Soares KC, Nishimura L, de Almeida DF, Ferreira LC (1997) Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans. J Clin Microbiol 35:1521–1525

    PubMed  CAS  Google Scholar 

  25. Clinical and Laboratory Standards Institute (CLSI) (2009) Performance standards for antimicrobial disk susceptibility tests. Approved standard—tenth edition. CLSI document M02-A10. CLSI, Wayne, PA

    Google Scholar 

  26. Argudín MA, Mendoza MC, Méndez FJ, Martín MC, Guerra B, Rodicio MR (2009) Clonal complexes and diversity of exotoxin gene profiles in methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from patients in a Spanish hospital. J Clin Microbiol 47:2097–2105

    Article  PubMed  Google Scholar 

  27. Jarraud S, Mougel C, Thioulouse J, Lina G, Meugnier H, Forey F, Nesme X, Etienne J, Vandenesch F (2002) Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect Immun 70:631–641

    Article  PubMed  CAS  Google Scholar 

  28. Monecke S, Slickers P, Ehricht R (2008) Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition. FEMS Immunol Med Microbiol 53:237–251

    Article  PubMed  CAS  Google Scholar 

  29. Shukla SK, Karow ME, Brady JM, Stemper ME, Kislow J, Moore N, Wroblewski K, Chyou PH, Warshauer DM, Reed KD, Lynfield R, Schwan WR (2010) Virulence genes and genotypic associations in nasal carriage, community-associated methicillin-susceptible and methicillin-resistant USA400 Staphylococcus aureus isolates. J Clin Microbiol 48:3582–3592

    Article  PubMed  CAS  Google Scholar 

  30. Argudín MA, Mendoza MC, Vázquez F, Guerra B, Rodicio MR (2011) Molecular typing of Staphylococcus aureus bloodstream isolates from geriatric patients attending a long-term care Spanish hospital. J Med Microbiol 60:172–179

    Article  PubMed  Google Scholar 

  31. Lee MA, Squirrell DJ, Leslie DL, Brown T (2009) Homogeneous fluorescent chemistries for real-time PCR. In: Logan J, Edwards K, Saunders N (eds) Real-time PCR: current technology and applications. Caister Academic Press, Norwich, UK, pp 31–70

    Google Scholar 

  32. Gomes AR, Westh H, de Lencastre H (2006) Origins and evolution of methicillin-resistant Staphylococcus aureus clonal lineages. Antimicrob Agents Chemother 50:3237–3244

    Article  PubMed  CAS  Google Scholar 

  33. Preney L, Caillon J, Vaucel J, Ygout JF, Donnio PY; BCB MRSA Study Group (2005) Geographic dissemination of methicillin-resistant Staphylococcus aureus strains: a French regional study. Pathol Biol (Paris) 53:4–8

    Article  CAS  Google Scholar 

  34. Shittu AO, Lin J (2006) Antimicrobial susceptibility patterns and characterization of clinical isolates of Staphylococcus aureus in KwaZulu-Natal province, South Africa. BMC Infect Dis 6:125

    Article  PubMed  Google Scholar 

  35. Valaperta R, Tejada MR, Frigerio MA, Moroni A, Ciulla E, Cioffi S, Capelli P, Costa E (2010) Staphylococcus aureus nosocomial infections: the role of a rapid and low-cost characterization for the establishment of a surveillance system. New Microbiol 33:223–232

    PubMed  Google Scholar 

  36. Cotton MF, Wasserman E, Smit J, Whitelaw A, Zar HJ (2008) High incidence of antimicrobial resistant organisms including extended spectrum beta-lactamase producing Enterobacteriaceae and methicillin-resistant Staphylococcus aureus in nasopharyngeal and blood isolates of HIV-infected children from Cape Town, South Africa. BMC Infect Dis 8:40

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported, in part, by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Author information

Authors and Affiliations

  1. Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-902, Brazil

    C. O. Beltrame, A. M. N. Botelho, M. C. Silva-Carvalho, R. R. Souza, R. R. Bonelli, M. S. Ramundo, M. A. Guimarães, L. R. Coelho & A. M. S. Figueiredo

Authors
  1. C. O. Beltrame
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. N. Botelho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. C. Silva-Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. R. Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. R. Bonelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. S. Ramundo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. A. Guimarães
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L. R. Coelho
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. M. S. Figueiredo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. M. S. Figueiredo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beltrame, C.O., Botelho, A.M.N., Silva-Carvalho, M.C. et al. Restriction modification (RM) tests associated to additional molecular markers for screening prevalent MRSA clones in Brazil. Eur J Clin Microbiol Infect Dis 31, 2011–2016 (2012). https://doi.org/10.1007/s10096-011-1534-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-011-1534-1

Keywords

Search

Navigation