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A comparative study of two different methods of sample preparation for positive blood cultures for the rapid identification of bacteria using MALDI-TOF MS

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European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has lately been implemented as a solid technology for rapid microorganism identification in microbiology laboratories. This study compares two methods for bacterial separation from 85 positive blood culture before MALDI-TOF MS: (1) a conventional method that we used in our laboratory to prepare bacteria for susceptibility testing and (2) a new commercialized technique (Sepsityper). There were no significant differences in the identification of Gram-negative bacilli regardless of the bacterial separation method used. However, identification was greater for Gram-positive cocci when the Sepsityper method was used (84.15% vs. 100% in the identification to a genus level in staphylococci and 57.14% vs. 85.71% in the identification to a genus level of enterococci with the in-house and Sepsityper methods, respectively). Therefore, the Sepsityper method to prepare bacteria from a positive blood culture is more adequate for the further identification of Gram-positive cocci by MALDI-TOF MS.

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Acknowledgments

This study has been supported by 2009 SGR 1256 from the Departament de Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya, and by the Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Spanish Network for the Research in Infectious Disease (REIPI 06/0008). This work has also been supported by funding from the European Community (TROCAR contract HEALTH-F3-2008-223031) to J.V.

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Authors and Affiliations

  1. Department of Clinical Microbiology, Hospital Clinic, CRESIB/IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain

    P. M. Juiz, M. Almela, C. Melción, I. Campo, C. Esteban, C. Pitart, F. Marco & J. Vila

  2. Servei de Microbiologia, Hospital Clinic de Barcelona, Villarroel, 170, 08036, Barcelona, Spain

    J. Vila

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  1. P. M. Juiz
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  2. M. Almela
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  3. C. Melción
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  4. I. Campo
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  5. C. Esteban
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  6. C. Pitart
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  7. F. Marco
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Correspondence to J. Vila.

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Juiz, P.M., Almela, M., Melción, C. et al. A comparative study of two different methods of sample preparation for positive blood cultures for the rapid identification of bacteria using MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 31, 1353–1358 (2012). https://doi.org/10.1007/s10096-011-1449-x

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  • DOI: https://doi.org/10.1007/s10096-011-1449-x

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