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Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS

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Abstract

The identification of Salmonella sp. in stool samples usually takes 2 days when employing routine procedures. Fast approaches are necessary in order to shorten the analysis time. The aim of this work was the development of a rapid procedure for the detection of Salmonella sp. from clinical stool samples. Spiked stool samples were cultured in selective selenite enrichment broth. Identifications were directly performed from the liquid broth by the MALDI Biotyper. After the evaluation of this method, the same procedure was applied to clinical samples. Coevally, the samples were streaked on Hektoen agar and single colonies were analyzed by the MALDI Biotyper. For comparison, the liquid broth was plated according to the standard laboratory procedure. A total of 4,847 samples were analyzed for Salmonella sp. In total, 108 Salmonella sp.-positive samples were identified; 66 of these were identified after the streaking of stool samples on Hektoen agar and subsequent MALDI Biotyper analysis of Salmonella sp. suspicious colonies. These and a further 34 samples were detected as Salmonella sp.-positive directly from the selenite enrichment broth on day one. Eight Salmonella sp.-positive samples were not detected before plating of the selenite broth and subsequent MALDI Biotyper analysis on day two. The combination of MALDI Biotyper analysis and selective selenite enrichment broth identification delivers positive results for the majority of the samples already after one day.

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Acknowledgment

The authors are grateful to Dr. Markus Timke for his helpful discussion of the manuscript and Ms. Erika Tranfield for the careful proofreading.

Transparency declaration

The authors Katrin Sparbier and Markus Kostrzewa are employed by the mass spectrometry company Bruker Daltonik GmbH.

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Authors and Affiliations

  1. Bruker Daltonik GmbH, Fahrenheitstr. 4, 28359, Bremen, Germany

    K. Sparbier & M. Kostrzewa

  2. Praxis für Laboratoriumsmedizin, Ärztliche Gemeinschaft für Diagnostik Köln-Bonn, Hauptstr. 71–73, 50996, Köln-Rodenkirchen, Germany

    U. Weller & C. Boogen

Authors
  1. K. Sparbier
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  2. U. Weller
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  3. C. Boogen
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  4. M. Kostrzewa
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Correspondence to K. Sparbier.

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Katrin Sparbier and Ulrich Weller equally contributed to this work.

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Sparbier, K., Weller, U., Boogen, C. et al. Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 31, 767–773 (2012). https://doi.org/10.1007/s10096-011-1373-0

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  • DOI: https://doi.org/10.1007/s10096-011-1373-0

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