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Revisited distribution of nonfermenting Gram-negative bacilli clinical isolates

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Abstract

Nonfermenting Gram-negative bacilli (NF-GNB) are ubiquitous environmental opportunistic bacteria frequently misidentified by conventional phenotypic methods. The aim of this study was to determine the distribution of NF-GNB species by 16 S rRNA gene sequencing (used as reference method) and to compare performances of biochemical tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). From nine French hospitals, 188 NF-GNB isolates (except P. aeruginosa and A. baumannii) were prospectively collected from 187 clinical samples between December 2008 and May 2009. By using the genotypic approach, 173 (92%) and 188 (100%) isolates were identified to the species and genus level, respectively. They covered 35 species and 20 genera, with a predominance of Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Pseudomonas putida group bacteria. Of the 173 species-level identified strains, concordant identification to the species-level was obtained for 75.1%, 83% and 88.9% of isolates with API 20 NE strip, the VITEK-2 (ID-GN card) system and MALDI-TOF-MS, respectively. By excluding S. maltophilia isolates accurately identified by the three methods, genus-level identification was much higher for MALDI-TOF-MS (92.9%), compared with API 20 NE and VITEK-2 (76.2% and 80.8%, respectively). In conclusion, MALDI-TOF-MS represents a rapid, inexpensive, and accurate tool for routine identification of NF-GNB in human clinical samples.

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Acknowledgements

We thank Pr. X. Nassif for access to MALDI-TOF-MS and Andromas@Software. We are grateful to Michel Auzou (Caen), Brigitte Carzon (Versailles), and Brunhilde Dauphin (Andromas) for excellent technical assistance.

Transparency declaration

The authors declare that they have no conflicts of interest.

Author information

Authors and Affiliations

  1. Service de Bactériologie-Virologie, Groupe Hospitalier Lariboisière-Fernand Widal, Assistance Publique-Hôpitaux de Paris, 2 rue Ambroise Paré, 75475, Paris Cedex 10, France

    H. Jacquier

  2. Laboratoire de Microbiologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France

    E. Carbonnelle

  3. Faculté de Médecine, Université Paris Descartes, Paris, France

    E. Carbonnelle, E. Bille & J. R. Zahar

  4. Laboratoire de Bactériologie-Hygiène, CHU de Nantes, Nantes, France

    S. Corvec & M. Illiaquer

  5. EA3826 Thérapeutiques cliniques et expérimentales des infections, UFR de Médecine, Université de Nantes, 44000, Nantes, France

    S. Corvec & M. Illiaquer

  6. Laboratoire de Microbiologie, CH de Versailles, Université Paris-Sud, Le Chesnay, France

    A. Le Monnier

  7. Service de Bactériologie-Virologie-Hygiène, Hôpital Necker, AP-HP, Paris, France

    E. Bille, J. R. Zahar & J. L. Beretti

  8. Service de Bactériologie-Virologie-Hygiène, Hôpital Avicenne, AP-HP, Université Paris 13, Bobigny, France

    F. Jauréguy

  9. Service de Microbiologie-Hygiène, Hôpital Louis Mourier, AP-HP, Colombes, France

    V. Fihman

  10. Laboratoire de Bactériologie-Virologie, Hôpital Saint-Antoine, AP-HP, Université Paris 6, Paris, France

    J. Tankovic

  11. Laboratoire de Microbiologie, CHU Côte de Nacre, Caen, France

    V. Cattoir

  12. Faculté de Médecine de Caen, Université de Caen Basse-Normandie, Caen, France

    V. Cattoir

  13. Faculté de Médecine, Université Paris 7–Denis Diderot, Paris, France

    H. Jacquier

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  1. H. Jacquier
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  2. E. Carbonnelle
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  3. S. Corvec
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Correspondence to H. Jacquier.

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The authors H. Jacquier and E. Carbonnelle are treated as first authors.

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Jacquier, H., Carbonnelle, E., Corvec, S. et al. Revisited distribution of nonfermenting Gram-negative bacilli clinical isolates. Eur J Clin Microbiol Infect Dis 30, 1579–1586 (2011). https://doi.org/10.1007/s10096-011-1263-5

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  • DOI: https://doi.org/10.1007/s10096-011-1263-5

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