Skip to main content

Advertisement

SpringerLink
Log in

Differentiating the growing nosocomial infectious threats Ralstonia pickettii and Ralstonia insidiosa

  • Article
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

Differentiation of the growing nosocomial infectious threats, Ralstonia pickettii and Ralstonia insidiosa, based on nitrate reduction, desferrioxamine susceptibility, arabinose, N-acetyl-glucosamine and phenylacetate assimilation is described. These tests can be used for preliminary identification of Ralstonia pickettii and Ralstonia insidiosa resulting in more accurate identification of these species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adley CC, Saieb FM (2005) Comparison of bioMerieux API 20NE and Remel RapID NF Plus, identification systems of type strains of Ralstonia pickettii. Lett Appl Microbiol 41(2):136–140

    Article  PubMed  CAS  Google Scholar 

  2. Blazevic DJ, Ederer GM (1975) Principles of biochemical tests in diagnostic microbiology. Wiley, USA

    Google Scholar 

  3. Coenye T, Goris J, De Vos P, Vandamme P, LiPuma JJ (2003) Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov. Int J Syst Evol Microbiol 53(Pt 4):1075–1080

    Article  PubMed  CAS  Google Scholar 

  4. Coenye T, Vandamme P, LiPuma JJ (2002) Infection by Ralstonia species in cystic fibrosis patients: identification of R. pickettii and R. mannitolilytica by polymerase chain reaction. Emerg Infect Dis 8(7):692–696

    PubMed  CAS  Google Scholar 

  5. Gilligan PH, Lum G, Vandamme PAR, Whittier S (2003) Burkholderia, Stenotrophomonas, Ralstonia, Brevundimonas, Comamonas, Delftia, Pandoraea and Acidovorax. In: Murray PR, Baron EJ, Pfaller MA, Jorgensen JH, Yolken RH (eds) Manual of Clinical Microbiology, 8th edn. ASM Press, Washington, D.C., pp 729–748

    Google Scholar 

  6. Glass MB, Popovic T (2005) Preliminary evaluation of the API 20NE and RapID NF plus systems for rapid identification of Burkholderia pseudomallei and B. mallei. J Clin Microbiol 43(1):479–483

    Article  PubMed  CAS  Google Scholar 

  7. Hoefel D, Monis PT, Grooby WL, Andrews S, Saint CP (2005) Profiling bacterial survival through a water treatment process and subsequent distribution system. J Appl Microbiol 99(1):175–186

    Article  PubMed  CAS  Google Scholar 

  8. Labarca JA, Trick WE, Peterson CL, Carson LA, Holt SC, Arduino MJ, Meylan M, Mascola L, Jarvis WR (1999) A multistate nosocomial outbreak of Ralstonia pickettii colonization associated with an intrinsically contaminated respiratory care solution. Clin Infect Dis 29(5):1281–1286

    Article  PubMed  CAS  Google Scholar 

  9. Laffineur K, Janssens M, Charlier J, Avesani V, Wauters G, Delmee M (2002) Biochemical and susceptibility tests useful for identification of nonfermenting gram-negative rods. J Clin Microbiol 40(3):1085–1087

    Article  PubMed  CAS  Google Scholar 

  10. Roberts LA, Collignon PJ, Cramp VB, Alexander S, McFarlane AE, Graham E, Fuller A, Sinickas V, Hellyar A (1990) An Australia-wide epidemic of Pseudomonas pickettii bacteraemia due to contaminated "sterile" water for injection. Med J Aust 152(12):652–655

    PubMed  CAS  Google Scholar 

  11. Ryan MP, Pembroke JT, Adley CC (2006) Ralstonia pickettii: a persistent gram-negative nosocomial infectious organism. J Hosp Infect 62(3):278–284

    Article  PubMed  CAS  Google Scholar 

  12. Ryan MP (2009) Genotypic and phenotypic analysis of Ralstonia pickettii high purity water isolates. Thesis, University of Limerick, Limerick, Ireland

  13. van Pelt C, Verduin CM, Goessens WH, Vos MC, Tummler B, Segonds C, Reubsaet F, Verbrugh H, van Belkum A (1999) Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods. J Clin Microbiol 37(7):2158–2164

    PubMed  Google Scholar 

  14. Vaneechoutte M, Kampfer P, De Baere T, Falsen E, Verschraegen G (2004) Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov. Int J Syst Evol Microbiol 54(Pt 2):317–327

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Department of Chemical and Environmental Sciences, Faculty of Science and Engineering, University of Limerick.

Author information

Authors and Affiliations

  1. Microbiology Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland

    M. P. Ryan & C. C. Adley

  2. Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland

    J. T. Pembroke

Authors
  1. M. P. Ryan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. T. Pembroke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. C. Adley
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. C. Adley.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ryan, M.P., Pembroke, J.T. & Adley, C.C. Differentiating the growing nosocomial infectious threats Ralstonia pickettii and Ralstonia insidiosa . Eur J Clin Microbiol Infect Dis 30, 1245–1247 (2011). https://doi.org/10.1007/s10096-011-1219-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-011-1219-9

Keywords

Search

Navigation