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Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay

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Abstract

Rapid molecular typing methods can be a valuable aid in the investigation of suspected outbreaks. We used a semi-automated repetitive sequence-based polymerase chain reaction (Rep-PCR) typing assay and pulsed field gel electrophoresis (PFGE) to investigate the relationship between local Klebsiella pneumoniae (K. pneumoniae) producing extended spectrum β-lactamases (ESBLs) and their relation to recognized Danish outbreak strains. PFGE and Rep-PCR produced similar clustering among isolates. Individual isolates from each cluster were further characterized by PCR amplification and sequencing of bla TEM, bla SHV, and bla CTX-M, and multilocus sequence typing (MLST). Thirty-five out of 52 ESBL-producing K. pneumoniae isolates were ST15 and bla CTX-M15, bla SHV-28, and bla TEM-1 positive by PCR. Ten out of 52 were ST16 and tested positive for bla CTX-M15, bla SHV-1, and bla TEM-1. Isolates from previously recognized hospital outbreaks were also ST15 and PCR positive for bla CTX-M15, bla SHV-28, and bla TEM-1, and typed within the main cluster by both Rep-PCR and PFGE. In conclusion, K. pneumoniae ST15 containing bla CTX-M15 and bla SHV-28 constitutes an epidemic clone in the Copenhagen area and this clone can be rapidly recognized by semi-automated Rep-PCR.

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Acknowledgment

The authors gratefully acknowledge support from bioMérieux for providing access to the Agilent 2100 Bioanalyzer.

Transparency declaration

This work was supported by Fondation Idella (grants 3.3.5.-2007 and 3.3.5/2008/II to K.S.). All authors declare that they do not have any commercial interests or associations that pose a conflict of interest with regard to this article.

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Authors and Affiliations

  1. Department of Clinical Microbiology 445, Hvidovre Hospital, Kettegård Alle 30, 2650, Hvidovre, Denmark

    J. B. Nielsen, R. L. Jørgensen & K. Schønning

  2. Department of Clinical Microbiology, Odense University Hospital, 5000, Odense, Denmark

    M. N. Skov

  3. Department of Clinical Microbiology, Næstved Sygehus, 4700, Næstved, Denmark

    O. Heltberg

  4. Department of Clinical Microbiology, Hillerød Hospital, 3400, Hillerød, Denmark

    D. S. Hansen

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  1. J. B. Nielsen
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  2. M. N. Skov
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  3. R. L. Jørgensen
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  4. O. Heltberg
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  5. D. S. Hansen
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  6. K. Schønning
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Correspondence to K. Schønning.

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Nielsen, J.B., Skov, M.N., Jørgensen, R.L. et al. Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay. Eur J Clin Microbiol Infect Dis 30, 773–778 (2011). https://doi.org/10.1007/s10096-011-1153-x

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  • DOI: https://doi.org/10.1007/s10096-011-1153-x

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