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Significance of methicillin–teicoplanin resistant Staphylococcus haemolyticus in bloodstream infections in patients of the Semmelweis University hospitals in Hungary

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Abstract

The purpose of this study was to quantify the impact of Staphylococcus haemolyticus in the epidemiology of the blood stream infection (BSI) and to characterize the rates and quantitative levels of resistance to antistaphylococcal drugs. During an eight-year period, 2967 BSIs of the patients hospitalized in different clinical departments of the Semmelweis University, Budapest, Hungary were analyzed. One hundred eighty-four were caused by S. haemolyticus, amounting to 6% of all infections. The antibacterial resistance of S. haemolyticus isolates was investigated by the broth microdilution method, vancomycin agar screen, population analysis profile and PCR for mecA, vanA and vanB genes detection. Epidemiological investigation was processed by determining phenotypic antibiotic resistance patterns and PFGE profiles. Extremely high MIC levels of resistance were obtained to oxacillin, erythromycin, clindamycin, gentamicin and ciprofloxacin. The incidence of teicoplanin reduced susceptibility revealed 32% without possessing either the vanA or vanB gene by the strains. PFGE revealed 56 well-defined genotypes indicating no clonal relationship of the strains. The propensity of S. haemolyticus to acquire resistance and its pathogenic potential in immunocompromised patients, especially among preterm neonates, emphasise the importance of species level identification of coagulase-negative staphylococci and routinely determine the MIC of proper antibacterial agents for these isolates.

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Acknowledgements

This work was supported by the Hungarian National Scientific Research Found (OTKA) Grant Numbers: T32473, M36764 and T46186 to Ferenc Rozgonyi.

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Author notes

  1. V. Cser

    Present address: Microbiology Diagnostic Laboratory, United Saint István and Saint László Municipal Hospital, Budapest, Hungary

Authors and Affiliations

  1. Division of Microbiological Diagnostic Laboratory, Central Clinical Laboratory, Semmelweis University, Nagyvárad tér 4, 1089, Budapest, Hungary

    K. Kristóf & E. Kocsis

  2. Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary

    D. Szabó, S. Kardos, V. Cser & K. Nagy

  3. Department of Prosthodontics, Semmelweis University, Budapest, Hungary

    P. Hermann

  4. Microbiology Laboratory, Department of Dermatology, Venerology and Dermatooncology, Semmelweis University, Budapest, Hungary

    F. Rozgonyi

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  1. K. Kristóf
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Kristóf, K., Kocsis, E., Szabó, D. et al. Significance of methicillin–teicoplanin resistant Staphylococcus haemolyticus in bloodstream infections in patients of the Semmelweis University hospitals in Hungary. Eur J Clin Microbiol Infect Dis 30, 691–699 (2011). https://doi.org/10.1007/s10096-010-1142-5

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