Abstract
The aim of this study was to perform the molecular characterization of methicillin-resistant Staphylococcus haemolyticus (MRSH) from clinical specimens of patients in a Chinese hospital. One hundred and thirty-three strains of S. haemolyticus collected from April 2002 to April 2003 were analyzed. Antimicrobial susceptibility to 15 antimicrobial agents was determined by the broth microdilution method. The resistant rates to penicillin G and oxacillin were higher than 90%. There were no isolates resistant to linezolid or vancomycin, and only 6.0% of the strains were resistant to teicoplanin. The positivity rate for mecA genes was 90.2% by polymerase chain reaction (PCR). Ninety MRSH (isolated from inpatients and mecA-gene-positive) were genotyped by pulsed-field gel electrophoresis (PFGE) after SmaI digestion. Twenty-five different PFGE patterns (A∼Y) were found and a major clone (type A; n = 36) with five subtypes was identified. Clone A was detected during a 1-year period. Identical PFGE types were found in different wards and patients. The results of this study suggest the clonal spread of MRSH within our hospital. This emphasizes the need for control and prevention measures.
References
Kloos WE, Bannerman TL (1994) Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev 7(1):117–140
Falcone M, Micozzi A, Pompeo ME, Baiocchi P, Fabi F, Penni A, Martino P, Venditti M (2004) Methicillin-resistant staphylococcal bacteremia in patients with hematologic malignancies: clinical and microbiological retrospective comparative analysis of S. haemolyticus, S. epidermidis and S. epidermidis. J Chemother 16(6):540–548
Raponi G, Ghezzi MC, Gherardi G, Dicuonzo G, Caputo D, Venditti M, Rocco M, Micozzi A, Mancini C (2005) Antimicrobial susceptibility, biochemical and genetic profiles of Staphylococcus haemolyticus strains isolated from the bloodstream of patients hospitalized in critical care units. J Chemother 17(3):264–269
Santos Sanches I, Mato R, de Lencastre H, Tomasz A (2000) Patterns of multidrug resistance among methicillin-resistant hospital isolates of coagulase-positive and coagulase-negative staphylococci collected in the international multicenter study RESIST in 1997 and 1998. Microb Drug Resist 6(3):199–211
Mayhall CG (2004) Hospital epidemiology and infection control. Lippincott Wiliams and Wilkins, Philadelphia, pp 495–510
Clinical and Laboratory Standards Institute (CLSI) (2006) Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. CLSI document M100-S16, vol. 26-3; M7-A7, vol. 26-2; M2-A9, vol. 26-1. CLSI, Wayne, PA
Degener JE, Heck ME, van Leeuwen WJ, Heemskerk C, Crielaard A, Joosten P, Caesar P (1994) Nosocomial infection by Staphylococcus haemolyticus and typing methods for epidemiological study. J Clin Microbiol 32(9):2260–2265
Biavasco F, Vignaroli C, Lazzarini R, Varaldo PE (2000) Glycopeptide susceptibility profiles of Staphylococcus haemolyticus bloodstream isolates. Antimicrob Agents Chemother 44(11):3122–3126
Song MD, Wachi M, Doi M, Ishino F, Matsuhashi M (1987) Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Lett 221(1):167–171
Prevost G, Jaulhac B, Piemont Y (1992) DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol 30(4):967–973
Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33(9):2233–2239
Campanile F, Bongiorno D, Borbone S, Falcone M, Giannella M, Venditti M, Stefani S (2008) In vitro activity of daptomycin against methicillin- and multi-resistant Staphylococcus haemolyticus invasive isolates carrying different mec complexes. Diagn Microbiol Infect Dis 61(2):227–231
Mohammedi I, Malhière S, Robert D, Charveriat MA, Etienne J (2006) Linezolid therapy of bloodborne teicoplanin-resistant Staphylococcus haemolyticus. Infection 34(5):292–293
de Mattos EM, Teixeira LA, Alves VM, Rezenda e Resende CA, da Silva Coimbra MV, da Silva-Carvalho MC, Ferreira-Carvalho BT, Figueiredo AM (2003) Isolation of methicillin-resistant coagulase-negative staphylococci from patients undergoing continuous ambulatory peritoneal dialysis (CAPD) and comparison of different molecular techniques for discriminating isolates of Staphylococcus epidermidis. Diagn Microbiol Infect Dis 45(1):13–22
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yu, MH., Chen, YG., Yu, YS. et al. Antimicrobial resistance and molecular characterization of Staphylococcus haemolyticus in a Chinese hospital. Eur J Clin Microbiol Infect Dis 29, 613–616 (2010). https://doi.org/10.1007/s10096-010-0893-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10096-010-0893-3