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Molecular fingerprinting of Staphylococcus aureus from bone and joint infections

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European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

The objective of the study was to determine if a clonal complex (CC) of Staphylococcus aureus or certain virulence and adhesion factors were associated with infections of bones and prosthetic implants. One hundred and nineteen isolates were characterised using microarrays. There was no evidence for a single virulence factor or CC being causative for bone and implant infections. Isolates belonged to 20 different CCs, with CC8 (19.33%), CC45 (17.65%) and CC30 (12.61%) being dominant. Population structure and the relative abundances of virulence genes was similar to previously described isolates from healthy carriers. Differences to carrier isolates included a higher proportion of CC45, a lower proportion of CC15, as well as a higher abundance of sak (staphylokinase) among patient isolates. For 23 patients with infections of total knee or hip prosthetics, it was possible to simultaneously obtain nasal swabs. Fifteen (65.2%) carried S. aureus in their anterior nares. In nine of them (39.1%), isolates from the infection site were identical to carriage isolates. This suggests an elevated risk of infection for S. aureus carriers and the possibility of endogenous infection in a high proportion of them. Therefore, the pre-operative screening and eradication of S. aureus in patients receiving total joint prosthetics should be considered.

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Acknowledgements

The authors thank A. Ruppelt, H. Kanig, S. Kolewa, E. Müller, I. Engelmann and J. Sachtschal for their excellent technical assistance, as well as R. Schuster and T. Uhlig for developing the software used in the microarray analysis. We acknowledge Prof. E. Jacobs, Prof. G. Ehninger and E. Ermantraut for their support. We thank D. Rowe and W. Rudolph for critically reading the manuscript.

The study was funded by the Vice-Directorate for Research of the Faculty of Medicine “Carl Gustav Carus” (MedDrive program 2008).

Ralf Ehricht and Peter Slickers are employees of CLONDIAG.

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Authors and Affiliations

  1. Medical Clinic and Policlinic I, University Hospital Dresden, Fetscherstrasse 74, 01307, Dresden, Germany

    C. Luedicke

  2. CLONDIAG GmbH, Loebstedter Strasse 103–105, 07749, Jena, Germany

    P. Slickers & R. Ehricht

  3. Institute for Medical Microbiology and Hygiene, Faculty of Medicine “Carl Gustav Carus”, Technical University of Dresden, Fetscherstrasse 74, 01307, Dresden, Germany

    S. Monecke

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  1. C. Luedicke
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  2. P. Slickers
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  3. R. Ehricht
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  4. S. Monecke
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Correspondence to S. Monecke.

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Luedicke, C., Slickers, P., Ehricht, R. et al. Molecular fingerprinting of Staphylococcus aureus from bone and joint infections. Eur J Clin Microbiol Infect Dis 29, 457–463 (2010). https://doi.org/10.1007/s10096-010-0884-4

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  • DOI: https://doi.org/10.1007/s10096-010-0884-4

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