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Metabolic properties of lactobacilli in women experiencing recurring episodes of bacterial vaginosis with vaginal pH ≥ 5

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Abstract

While 60% of women experiencing recurring episodes of bacterial vaginosis (BV) with vaginal pH ≥ 5 are depleted of resident probiotic lactobacilli, the remainder carry one or more strains of lactobacilli. Their ability to make D-lactic acid is, however, low (3.94 ± 0.72 mM/L) compared to the D-lactic acid produced by strains from healthy vagina with vaginal pH ∼ 4 (8.04 ± 1.07 mM/L) culture supernatant of 0.5 McFarland concentration (P < 0.001).

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References

  1. Wang J (2000) Bacterial vaginosis. Prim Care Update Ob Gyns 7(5):181–185

    Article  PubMed  Google Scholar 

  2. Nugent RP, Krohn MA, Hillier SL (1991) Reliability of diagnosing bacterial vaginosis is improved by a standardized method of Gram stain interpretation. J Clin Microbiol 29(2):297–301

    CAS  PubMed  Google Scholar 

  3. Pospiech A, Neumann B (1995) A versatile quick-prep of genomic DNA from gram-positive bacteria. Trends Genet 11:217–218

    Article  CAS  PubMed  Google Scholar 

  4. Dubernet S, Desmasures N, Guéguen M (2002) A PCR-based method for identification of lactobacilli at the genus level. FEMS Microbiol Lett 214:271–275

    Article  CAS  PubMed  Google Scholar 

  5. Song Y-L, Kato N, Liu C-X, Matsumiya Y, Kato H, Watanabe K (2000) Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiol Lett 187:167–173

    CAS  PubMed  Google Scholar 

  6. Tynkkynen S, Satokari R, Saarela M, Mattila-Sandholm T, Saxelin M (1999) Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains. Appl Environ Microbiol 65:3908–3914

    CAS  PubMed  Google Scholar 

  7. Edwards U, Rogall T, Blöcker H, Emde M, Böttger EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853

    Article  CAS  PubMed  Google Scholar 

  8. Garg KB, Ganguli I, Das R, Talwar GP (2009) Spectrum of Lactobacillus species present in healthy vagina of Indian women. Ind J Med Res 129:652–657

    CAS  Google Scholar 

  9. Rabe LK, Hillier SL (2003) Optimization of media for detection of hydrogen peroxide production by Lactobacillus species. J Clin Microbiol 41:3260–3264

    Article  CAS  PubMed  Google Scholar 

  10. Gutmann I, Wahlefeld AW (1974) In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 1464–1468

    Google Scholar 

Download references

Acknowledgements

We express our sincere appreciation to all of the women who participated in the study.

This study was supported by a Research Grant of the Department of Biotechnology (DBT), Govt. of India. K.B.G. is a recipient of the University Grants Commission (UGC) Fellowship.

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Correspondence to G. P. Talwar.

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Garg, K.B., Ganguli, I., Kriplani, A. et al. Metabolic properties of lactobacilli in women experiencing recurring episodes of bacterial vaginosis with vaginal pH ≥ 5. Eur J Clin Microbiol Infect Dis 29, 123–125 (2010). https://doi.org/10.1007/s10096-009-0818-1

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  • DOI: https://doi.org/10.1007/s10096-009-0818-1

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