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Retrospective analysis of the genetic diversity of Klebsiella oxytoca isolated in Poland over a 50-year period

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Abstract

Population genetics analyses and determination of the phylogenetic relationships between strains have proven to be extremely useful approaches, enabling deeper insights into the epidemiological pattern of bacterial species. There is no longitudinal data describing the molecular epidemiology of Klebsiella oxytoca strains that are opportunistic pathogens responsible for an increasing number of multi-resistant infections in hospitals. The aim of the present study was to assess the genetic diversity of K. oxytoca strains over a 50-year period using internal transcribed spacer polymerase chain reaction (ITS-PCR) and PCR MP (ang. PCR melting profiles) genotyping methods on a large collection of strains isolated from the patients of several hospitals in Poland. The phylogenetic analysis based on ITS-PCR exhibited six distinct branches. Two main groups, KoX and KoY, with four and two sub-groups within KoX and KoY, respectively, have been identified. Typing by the PCR MP method showed a higher level of genetic diversity. However, all K. oxytoca strains were also divided into six genotype groups (KoA, KoB, KoC, KoD, KoE and KoF). In conclusion, we found that the ITS-PCR and PCR MP methods are useful for the phylogenetic delineation of genetic groups in K. oxytoca.

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References

  1. Boucher HW, Talbot GH, Bradley JS, Edwards JE, Gilbert D, Rice LB, Scheld M, Spellberg B, Bartlett J (2009) Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clin Infect Dis 48:1–12

    Article  PubMed  Google Scholar 

  2. Podschun R, Ullmann U (1998) Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev 11:589–603

    PubMed  CAS  Google Scholar 

  3. Livermore DM (1995) β-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev 8:557–584

    PubMed  CAS  Google Scholar 

  4. Granier SA, Leflon-Guibout V, Goldstein FW, Nicolas-Chanoine MH (2003) New Klebsiella oxytoca β-lactamase genes bla OXY-3 and bla OXY-4 and a third genetic group of K. oxytoca based on bla OXY-3. Antimicrob Agents Chemother 47:2922–2928

    Article  PubMed  CAS  Google Scholar 

  5. Jonas D, Spitzmüller B, Daschner FD, Verhoef J, Brisse S (2004) Discrimination of Klebsiella pneumoniae and Klebsiella oxytoca phylogenetic groups and other Klebsiella species by use of amplified fragment length polymorphism. Res Microbiol 155:17–23

    Article  PubMed  CAS  Google Scholar 

  6. Fevre C, Jbel M, Passet V, Weill F-X, Grimont PAD, Brisse S (2005) Six groups of the OXY β-lactamase evolved over millions of years in Klebsiella oxytoca. Antimicrob Agents Chemother 49:3453–3462

    Article  PubMed  CAS  Google Scholar 

  7. Ørskov I, Ørskov F (1984) Serotyping of Klebsiella. In: Bergan T (ed) Methods in microbiology, vol 14. Academic Press Inc., New York, NY, pp 143–164

    Google Scholar 

  8. Jensen MA, Webster JA, Straus N (1993) Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol 59:945–952

    PubMed  CAS  Google Scholar 

  9. Krawczyk B, Samet A, Leibner J, Śledzińska A, Kur J (2006) Evaluation of a PCR melting profile technique for bacterial strain differentiation. J Clin Microbiol 44:2327–2332

    Article  PubMed  CAS  Google Scholar 

  10. Brisse S, Verhoef J (2001) Phylogenetic diversity of Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates revealed by randomly amplified polymorphic DNA, gyrA and parC genes sequencing and automated ribotyping. Int J Syst Evol Microbiol 51:915–924

    PubMed  CAS  Google Scholar 

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Acknowledgements

This work was supported by a grant from the State Committee for Scientific Research, Poland. We are grateful to Alicja Sekowska, Joanna Wroblewska and Eugenia Gospodarek for providing some of the Klebsiella oxytoca clinical strains.

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Authors and Affiliations

  1. Department of Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-233, Gdańsk, Poland

    K. Stojowska, B. Krawczyk & J. Kur

  2. Department of Bacteriology, National Institute of Public Health, National Institute of Hygiene, ul. Chocimska 24, 00-791, Warszawa, Poland

    S. Kałużewski

Authors
  1. K. Stojowska
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  2. B. Krawczyk
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  3. S. Kałużewski
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  4. J. Kur
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Corresponding author

Correspondence to J. Kur.

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Stojowska, K., Krawczyk, B., Kałużewski, S. et al. Retrospective analysis of the genetic diversity of Klebsiella oxytoca isolated in Poland over a 50-year period. Eur J Clin Microbiol Infect Dis 28, 1263–1266 (2009). https://doi.org/10.1007/s10096-009-0768-7

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  • DOI: https://doi.org/10.1007/s10096-009-0768-7

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