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Detection of Salmonella enterica serovar Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom

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Abstract

The aim of this study was to investigate the presence in the United Kingdom (UK) of Salmonella enterica serovar Typhimurium isolates carrying pUO-StVR2-like virulence-resistance hybrid plasmids that originated from pSLT. One hundred and fifty ampicillin-resistant isolates of S. Typhimurium, collected in different regions of the UK during 2006, were screened for the presence of bla OXA-1 carried by an InH-like integron (2000 bp/bla OXA-1-aadA1) characteristic of pUO-StVR2. Positive isolates were tested for the presence of a large plasmid that hybridised with probes specific for the bla OXA-1 and spvC genes, used as resistance and virulence markers of the hybrid plasmid, respectively. Eleven out of the 150 isolates fulfilled both criteria and were assigned to the S. Typhimurium pUO-StVR2 group. Nine were resistant to ampicillin, chloramphenicol, streptomycin/spectinomycin, sulfonamides and tetracycline, encoded by bla OXA-1, catA1, aadA1-like, sul1 and tet(B), respectively, and carried a pUO-StVR2-like plasmid of ca. 130 kb. Two contained hybrid plasmids of smaller size and lacked resistance(s) to chloramphenicol or chloramphenicol and tetracycline. The eleven isolates, which showed five and six closely related XbaI and BlnI profiles, respectively, were resistant to nitrofurantoin. In conclusion, multidrug-resistant S. Typhimurium isolates of the pUO-StVR2 group, which are endemic in Spain, were also detected in the UK, albeit with a low frequency (7.3%).

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Acknowledgements

This work was mostly performed during a short-term visit of A. Herrero at the Health Protection Agency (HPA). We are most grateful to the staff of the Health Protection Agency Centre for Infections, especially to Dr. K. Hopkins, for their valuable help. We also thank Dr. B. Guerra (Federal Institute for Risk Assessment, Berlin, Germany) for the sequencing of the gyr and par genes, helping with the hybridisation experiments and the useful comments. A. Herrero was the recipient of a grant from the Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología (FICYT, ref. BP03-014). The work in Spain was supported by project FIS PI080656 from the Spanish Ministry of Science and Innovation.

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Authors and Affiliations

  1. Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, Julián Clavería 6, 33006, Oviedo, Spain

    A. Herrero, M. C. Mendoza & M. R. Rodicio

  2. Salmonella Reference Unit, Laboratory of Enteric Pathogens, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London, NW 5EQ, UK

    E. J. Threlfall

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  1. A. Herrero
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  2. M. C. Mendoza
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  3. E. J. Threlfall
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  4. M. R. Rodicio
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Correspondence to M. R. Rodicio.

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Herrero, A., Mendoza, M.C., Threlfall, E.J. et al. Detection of Salmonella enterica serovar Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom. Eur J Clin Microbiol Infect Dis 28, 1087–1093 (2009). https://doi.org/10.1007/s10096-009-0753-1

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