Skip to main content

Advertisement

SpringerLink
Log in

Diagnostic value of the matrix metalloproteinase-8 rapid test for detecting microbial invasion of the amniotic cavity

  • Brief Report
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

The purpose of this paper was to compare the MMP-8 PTD Check (MPC) test with other indirect tests for detecting microbial invasion of the amniotic cavity (MIAC). Amniotic fluid (AF) was analyzed in 155 women for white blood cell (WBC) count, glucose concentration, and an MPC test and evaluated for MIAC using cultures for aerobic/anaerobic bacteria and mycoplasmas and polymerase chain reaction (PCR) of chlamydia. The median AF glucose concentration was lower and the median AF WBC count was higher in women with MIAC than in women without MIAC (p < 0.01 and p < 0.001, respectively). Also, the positive rate of the MPC test was higher in women with MIAC than in women without MIAC (p < 0.001). The sensitivities of AF glucose concentration, AF WBC count, and the MPC test for the detection of MIAC were 58.6%, 75.9%, and 86.2%, respectively. The specificities for the detection of MIAC were 76.2%, 80.2%, and 74.6%, respectively. We conclude that the MPC test is a rapid, easily performed, and accurate indirect method for detecting MIAC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Romero R, Sirtori M, Oyarzun E, Avila C, Mazor M, Callahan R, Sabo V, Athanassiadis AP, Hobbins JC (1989) Infection and labor. V. Prevalence, microbiology, and clinical significance of intraamniotic infection in women with preterm labor and intact membranes. Am J Obstet Gynecol 161:817–824

    PubMed  CAS  Google Scholar 

  2. Greig PC (1998) The diagnosis of intrauterine infection in women with preterm premature rupture of the membranes (PPROM). Clin Obstet Gynecol 41:849–863

    Article  PubMed  CAS  Google Scholar 

  3. Maymon E, Romero R, Pacora P, Gomez R, Athayde N, Edwin S, Yoon BH (2000) Human neutrophil collagenase (matrix metalloproteinase 8) in parturition, premature rupture of the membranes, and intrauterine infection. Am J Obstet Gynecol 183:94–99

    PubMed  CAS  Google Scholar 

  4. Nien JK, Yoon BH, Espinoza J, Kusanovic JP, Erez O, Soto E, Richani K, Gomez R, Hassan S, Mazor M, Edwin S, Bahado-Singh R, Romero R (2006) A rapid MMP-8 bedside test for the detection of intra-amniotic inflammation identifies patients at risk for imminent preterm delivery. Am J Obstet Gynecol 195:1025–1030

    Article  PubMed  Google Scholar 

  5. Gauthier DW, Meyer WJ (1992) Comparison of gram stain, leukocyte esterase activity, and amniotic fluid glucose concentration in predicting amniotic fluid culture results in preterm premature rupture of membranes. Am J Obstet Gynecol 167:1092–1095

    PubMed  CAS  Google Scholar 

  6. Yoon BH, Jun JK, Park KH, Syn HC, Gomez R, Romero R (1996) Serum C-reactive protein, white blood cell count, and amniotic fluid white blood cell count in women with preterm premature rupture of membranes. Obstet Gynecol 88:1034–1040

    Article  PubMed  CAS  Google Scholar 

  7. Romero R, Yoon BH, Mazor M, Gomez R, Gonzalez R, Diamond MP, Baumann P, Araneda H, Kenney JS, Cotton DB, Sehgal P (1993) A comparative study of the diagnostic performance of amniotic fluid glucose, white blood cell count, interleukin-6, and gram stain in the detection of microbial invasion in patients with preterm premature rupture of membranes. Am J Obstet Gynecol 169:839–851

    PubMed  CAS  Google Scholar 

  8. Maymon E, Romero R, Chaiworapongsa T, Berman S, Conoscenti G, Gomez R, Edwin S (2001) Amniotic fluid matrix metalloproteinase-8 in preterm labor with intact membranes. Am J Obstet Gynecol 185:1149–1155

    Article  PubMed  CAS  Google Scholar 

  9. Shim SS, Romero R, Jun JK, Moon KC, Kim G, Yoon BH (2005) C-reactive protein concentration in vaginal fluid as a marker for intra-amniotic inflammation/infection in preterm premature rupture of membranes. J Matern Fetal Neonatal Med 18:417–422

    Article  PubMed  CAS  Google Scholar 

  10. Yoon BH, Romero R, Moon JB, Shim SS, Kim M, Kim G, Jun JK (2001) Clinical significance of intra-amniotic inflammation in patients with preterm labor and intact membranes. Am J Obstet Gynecol 185:1130–1136

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Ulsan, College of Medicine, Asan Medical Center, 388-1 Poongnap-dong, Songpa-Gu, Seoul, 138-736, Korea

    S.-J. Lee, H.-S. Won, P. R. Lee, J.-Y. Shim & A. Kim

  2. Department of Laboratory Medicine, University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Korea

    M.-N. Kim

Authors
  1. S.-J. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H.-S. Won
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M.-N. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. R. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J.-Y. Shim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J.-Y. Shim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, SJ., Won, HS., Kim, MN. et al. Diagnostic value of the matrix metalloproteinase-8 rapid test for detecting microbial invasion of the amniotic cavity. Eur J Clin Microbiol Infect Dis 27, 1257–1260 (2008). https://doi.org/10.1007/s10096-008-0566-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-008-0566-7

Keywords

Search

Navigation