Abstract
The aim of this study was to measure the seroprevalence to mumps in Norwegian conscripts belonging to the first children vaccination cohorts that had been offered two doses of MMR vaccine. The seroprevalence to mumps was 76% with the Microimmune assay and 85% with the Enzygnost assay. We also compared the performance of the Microimmune assay for detection of mumps- and measles-specific IgG antibodies in 340 paired serum and oral fluid samples from the conscripts and evaluated the effect of revaccination. Mumps-specific IgG antibodies were detected in only 61% of the oral fluids. In contrast, high levels of measles-specific IgG antibodies were detected in both the serum and oral fluid samples. Based on these results, we are only able to recommend the use of oral fluid for surveillance of measles in Norway. Our results may also indicate that the seroprevalence necessary to interrupt transmission of mumps has not been reached in vaccinated young adult Norwegians. Seroconversion was observed in all initially measles seronegative conscripts after revaccination, whereas 23 of 27 initially mumps seronegative conscripts failed to seroconvert.
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References
Anonymous (2002) Measles and measles immunization in Norway; historical review and present situation. Eurosurveillance 6
MSIS (2007) Norwegian surveillance system for communicable diseases. http://www.msis.no/. Cited 17 September 2007
Vainio K, Samdal HH, Anestad G, Skutlaberg DH, Bransdal KT, Mundal R, Aaberge I (2007) Seroprevalence of measles among Norwegian military conscripts in 2004. Eur J Clin Microbiol Infect Dis 26:217–220
Parry JV, Perry KR, Mortimer PP (1987) Sensitive assays for viral antibodies in saliva: an alternative to tests on serum. Lancet 2:72–75
Backhouse JL, Gidding HF, McIntyre PB, Gilbert GL (2006) Evaluation of two enzyme immunoassays for detection of immunoglobulin G antibodies to mumps virus. Clin Vaccine Immunol 13:764–767
Garrido RM, Blanco QA, Garrote Adrados JA, Telleria Orriols JJ, Arranz SE (1997) Value of salivary antibodies for determining seropositivity to measles, rubella, and mumps in children and adults. An Esp Pediatr 47:499–504
Gill J, Aston R, Vyse AJ, White JM, Greenwood A (2002) Susceptibility of young offenders to measles and rubella: an antibody prevalence study using oral fluid samples. Commun Dis Public Health 5:314–317
Kremer JR, Muller CP (2005) Evaluation of commercial assay detecting specific immunoglobulin G in oral fluid for determining measles immunity in vaccinees. Clin Diagn Lab Immunol 12:668–670
Nigatu W, Nokes DJ, Enquselassie F, Brown DW, Cohen BJ, Vyse AJ, Cutts FT (1999) Detection of measles specific IgG in oral fluid using an FITC/anti-FITC IgG capture enzyme linked immunosorbent assay (GACELISA). J Virol Methods 83:135–144
Nokes DJ, Enquselassie F, Nigatu W, Vyse AJ, Cohen BJ, Brown DW, Cutts FT (2001) Has oral fluid the potential to replace serum for the evaluation of population immunity levels? A study of measles, rubella and hepatitis B in rural Ethiopia. Bull World Health Organ 79:588–595
Perry KR, Brown DW, Parry JV, Panday S, Pipkin C, Richards A (1993) Detection of measles, mumps, and rubella antibodies in saliva using antibody capture radioimmunoassay. J Med Virol 40:235–240
Thieme T, Piacentini S, Davidson S, Steingart K (1994) Determination of measles, mumps, and rubella immunization status using oral fluid samples. JAMA 272:219–221
Brown DW, Ramsay ME, Richards AF, Miller E (1994) Salivary diagnosis of measles: a study of notified cases in the United Kingdom, 1991–3. BMJ 308:1015–1017
Amela C, Pachon I, de Ory F (2003) Evaluation of the measles, mumps and rubella immunisation programme in Spain by using a sero-epidemiological survey. Eur J Epidemiol 18:71–79
Muhlemann K (2004) The molecular epidemiology of mumps virus. Infect Genet Evol 4:215–219
Nardone A, Pebody RG, van den Hof S, Levy-Bruhl D, Plesner AM, Rota MC, Tischer A, Andrews N, Berbers G, Crovari P, Edmunds WJ, Gabutti G, Saliou P, Miller E (2003) Sero-epidemiology of mumps in western Europe. Epidemiol Infect 131:691–701
Dominguez A, Plans P, Costa J, Torner N, Cardenosa N, Batalla J, Plasencia A, Salleras L (2006) Seroprevalence of measles, rubella, and mumps antibodies in Catalonia, Spain: results of a cross-sectional study. Eur J Clin Microbiol Infect Dis 25:310–317
Huerta M, Davidovitch N, Aboudy Y, Ankol OE, Balicer RD, Zarka S, Grotto I (2006) Declining population immunity to mumps among Israeli military recruits. Vaccine 24:6300–6303
Janaszek-Seydlitz W, Bucholc B, Gorska P, Slusarczyk J (2005) Mumps in Poland since 1990 to 2003; epidemiology and antibody prevalence. Vaccine 23:2711–2716
Mossong J, Putz L, Schneider F (2004) Seroprevalence of measles, mumps and rubella antibodies in Luxembourg: results from a national cross-sectional study. Epidemiol Infect 132:11–18
Vyse AJ, Gay NJ, Hesketh LM, Pebody R, Morgan-Capner P, Miller E (2006) Interpreting serological surveys using mixture models: the seroepidemiology of measles, mumps and rubella in England and Wales at the beginning of the 21st century. Epidemiol Infect 134:1303–1312
Gay NJ, Vyse AJ, Enquselassie F, Nigatu W, Nokes DJ (2003) Improving sensitivity of oral fluid testing in IgG prevalence studies: application of mixture models to a rubella antibody survey. Epidemiol Infect 130:285–291
Madar R, Straka S, Baska T (2002) Detection of antibodies in saliva—an effective auxiliary method in surveillance of infectious diseases. Bratisl Lek Listy 103:38–41
Nigatu W, Nokes DJ, Afework A, Brown DW, Cutts FT, Jin L (2006) Serological and molecular epidemiology of measles virus outbreaks reported in Ethiopia during 2000–2004. J Med Virol 78:1648–1655
Acknowledgments
This study was supported by the Norwegian Institute of Public Health and by the Norwegian Armed Forces. We thank H. Fremstad, W. Holmen, and A. Lund for excellent technical assistance. We also thank the remaining staff of our laboratory, I. J. Rodal and the military staff who participated in sample collection during the registration for primary military service. All experiments in this project comply with the current laws in Norway.
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Vainio, K., Samdal, H.H., Ånestad, G. et al. Detection of measles- and mumps-specific IgG antibodies in paired serum and oral fluid samples from Norwegian conscripts. Eur J Clin Microbiol Infect Dis 27, 461–465 (2008). https://doi.org/10.1007/s10096-008-0460-3
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DOI: https://doi.org/10.1007/s10096-008-0460-3