References
Clinical and Laboratory Standards Institute (2007) Performance standards for antimicrobial susceptibility testing: seventeenth informational supplement M100-S17. Clinical and Laboratory Standards Institute, Wayne, PA
Elsayed S, Hamilton N, Boyd D, Mulvey M (2001) Improved primer design for multiplex PCR analysis of vancomycin-resistant Enterococcus spp. J Clin Microbiol 39:2367–2368
Kariyama R, Mitsuhata R, Chow JW, Clewell DB, Kumon H (2000) Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin resistant enterococci. J Clin Microbiol 38:3092–3095
Dutka-Malen S, Evers S, Courvalin P (1995) Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33:24–27; erratum 33:1434
Patel R, Uhl JR, Kohner P, Hopkins MK, Cockerill FR III (1997) Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci. J Clin Microbiol 35:703–707
Drews SJ, Johnson G, Gharabaghi F, Roscoe M, Matlow A, Tellier R, Richardson SE (2006) A 24-hour screening protocol for identification of vancomycin resistant Enterococcus faecium. J Clin Microbiol 44:1578–1580
Palladino S, Kay ID, Flexman JP, Boehm I, Costa AMG, Lambert EJ, Christiansen KJ (2003) Rapid detection of vanA and vanB genes directly from clinical specimens and enrichment broths by real-time multiplex PCR assay. J Clin Microbiol 41:2483–2486
Paule SM, Trick WE, Tenover FC, Lankford M, Cunningham S, Stosor V, Cordell RL, Peterson LR (2003) Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci. J Clin Microbiol 41:4805–4807
Satake S, Clark N, Rimland D, Nolte FS, Tenover FC (1997) Detection of vancomycin-resistant enterococci in fecal samples by PCR. J Clin Microbiol 35:2325–2330
Sloan LM, Uhl JR, Vetter EA, Schleck CD, Harmsen WS, Manahan J, Thompson RL, Rosenblatt JE, Cockerill FR III (2004) Comparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin resistant enterococci from perianal swabs. J Clin Microbiol 42:2636–2643
Koh TH, Deepak RN, Se-Thoe SY, Lin RVTP, Koay ESC (2007) Experience with the Roche LightCycler VRE detection kit during a large outbreak of vanB2/B3 vancomycin-resistant Enterococcus faecium. J Antimicrob Chemother 60:182–183
Ballard SA, Grabsch EA, Johnson PDR, Grayson ML (2005) Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci: interference by vanB-containing anaerobic bacilli. Antimicrob Agents Chemother 49:77–81
Young HL, Ballard SA, Roffey P, Grayson ML (2007) Direct detection of vanB2 using the Roche LightCycler vanA/B detection assay to indicate vancomycin-resistant enterococcal carriage - sensitive but not specific. J Antimicrob Chemother 59:809–810
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Al-Mohri, H.A., Tadros, M.A., Louie, L. et al. Utility of direct, real-time PCR in the management of a nosocomial outbreak of vancomycin-resistant Enterococcus faecium (vanB genotype). Eur J Clin Microbiol Infect Dis 27, 321–322 (2008). https://doi.org/10.1007/s10096-007-0432-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10096-007-0432-z