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Evaluation of the immunochromatographic CORIS Giardia-Strip test for rapid diagnosis of Giardia lamblia

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Abstract

The aim of this study was to evaluate the performance of the CORIS Giardia-Strip test (CORIS Bioconcept, Gembloux, Belgium) as a rapid initial method for the routine diagnosis of giardiasis. Compared to a commercial ELISA-coproantigen test (ProSpect Giardia-ELISA-microplate assay; Remel, Lenexa, KS, USA), the commercial strip test had a sensitivity of 58%, a specificity of 99%, a positive predictive value of 93% and a negative predictive value of 93% (n=158). These results are comparable to those obtained using microscopy of direct wet-mounted stool. Since the CORIS Giardia-Strip test is simpler to perform, it can replace direct wet-mounted stool microscopy for the rapid diagnosis of giardiasis; however, its sensitivity is inferior to that of other immunochromatographic antigen detection tests and fresh stool samples are required for its use. Nevertheless, the results suggest that a positive CORIS Giardia-Strip test outcome does not need confirmation, while samples with negative results should be re-examined using another, more sensitive, test.

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References

  1. Adam RD (1991) The biology of Giardia spp. Microbiol Rev 55:706–732

    PubMed  CAS  Google Scholar 

  2. Shetty N, Prabhu T (1988) Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis. J Clin Pathol 41:694–699

    Article  PubMed  CAS  Google Scholar 

  3. Rosoff JD, Sanders CA, Sonnad SS, De Lay PR, Hadley WK, Vincenzi FF, Yajko DM, O’Hanley PD (1989) Stool diagnosis of giardiasis using a commercially available enzyme immunoassay to detect Giardia-specific antigen 65 (GSA 65). J Clin Microbiol 27:1997–2002

    PubMed  CAS  Google Scholar 

  4. Johnston SP, Ballard MM, Beach MJ, Causer L, Wilkins PP (2003) Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens. J Clin Microbiol 41:623–626 DOI 10.1128/JCM.41.2.623–626.2003

    Article  PubMed  Google Scholar 

  5. Garcia LS, Shimizu RY (1997) Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J Clin Microbiol 35:1526–1529

    PubMed  CAS  Google Scholar 

  6. Zimmerman SK, Needham CA (1995) Comparison of conventional stool concentration and preserved-smear methods with Merifluor Cryptosporidium/Giardia Direct Immunofluorescence Assay and ProSpecT Giardia EZ Microplate Assay for detection of Giardia lamblia. J Clin Microbiol 33:1942–1943

    PubMed  CAS  Google Scholar 

  7. Ghosh S, Debnath A, Sil A, De S, Chattopadhyay DJ, Das P (2000) PCR detection of Giardia lamblia in stool: targeting intergenic spacer region of multicopy rRNA gene. Mol Cell Probes 14:181–189 DOI 10.1006/mcpr.2000.0302

    Article  PubMed  CAS  Google Scholar 

  8. Verweij JJ, Schinkel J, Laeijendecker D, van Rooyen MA, van Lieshout L, Polderman AM (2003) Real-time PCR for the detection of Giardia lamblia. Mol Cell Probes 17:223-225 DOI 10.1016/S0890-8508(03)00057-4

    Article  PubMed  CAS  Google Scholar 

  9. Garcia LS, Shimizu RY (2000) Detection of Giardia lamblia and Cryptosporidium parvum antigens in human fecal specimens using the ColorPAC combination rapid solid-phase qualitative immunochromatographic assay. J Clin Microbiol 38:1267–1268

    PubMed  CAS  Google Scholar 

  10. Garcia LS, Shimizu RY, Bernard CN (2000) Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum antigens in human fecal specimens using the triage parasite panel enzyme immunoassay. J Clin Microbiol 38:3337–3340

    PubMed  CAS  Google Scholar 

  11. Garcia LS, Shimizu RY, Novak S, Carroll M, Chan F (2003) Commercial assay for detection of Giardia lamblia and Cryptosporidium parvum antigens in human fecal specimens by rapid solid-phase qualitative immunochromatography. J Clin Microbiol 41:209–212 DOI 10.1128/JCM.41.1.209–212.2003

    Article  PubMed  CAS  Google Scholar 

  12. Marti H, Escher E (1990) SAF—eine alternative Fixierlösung für parasitologische Stuhluntersuchungen. Schweiz Med Wochenschr 120:1473–1476

    PubMed  CAS  Google Scholar 

  13. Rocha MO, Mello RT, Guimaraes TM, Toledo V, Moreira M, Costa CA (1999) Detection of a Giardia lamblia coproantigen by using a commercially available immunoenzymatic assay, in Belo Horizonte, Brazil. Rev Inst Med Trop Sao Paulo 41:151–154

    PubMed  Google Scholar 

  14. Katanik MT, Schneider SK, Rosenblatt JE, Hall GS, Procop GW (2001) Evaluation of ColorPAC Giardia/Cryptosporidium rapid assay and ProSpecT Giardia/Cryptosporidium microplate assay for detection of Giardia and Cryptosporidium in fecal specimens. J Clin Microbiol 39:4523–4525 DOI 10.1128/JCM.39.12.4523–4525.2001

    Article  PubMed  CAS  Google Scholar 

  15. Aziz H, Beck CE, Lux MF, Hudson MJ (2001) A comparison study of different methods used in the detection of Giardia lamblia. Clin Lab Sci 14:150–154

    PubMed  CAS  Google Scholar 

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Acknowledgement

Financial support for the study was obtained from the Forschungsförderungs-Programm der Medizinischen Fakultät Heidelberg (FULEXVAR 2002).

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Authors and Affiliations

  1. Abteilung Parasitologie, Hygiene Institut, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany

    N. Oster, H. Gehrig-Feistel, H. Jung, J. Kammer, J. E. McLean & M. Lanzer

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  1. N. Oster
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  2. H. Gehrig-Feistel
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  3. H. Jung
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  4. J. Kammer
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  5. J. E. McLean
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  6. M. Lanzer
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Correspondence to M. Lanzer.

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Oster, N., Gehrig-Feistel, H., Jung, H. et al. Evaluation of the immunochromatographic CORIS Giardia-Strip test for rapid diagnosis of Giardia lamblia . Eur J Clin Microbiol Infect Dis 25, 112–115 (2006). https://doi.org/10.1007/s10096-006-0088-0

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  • DOI: https://doi.org/10.1007/s10096-006-0088-0

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