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Evaluation of the new Vitek 2 GN card for the identification of gram-negative bacilli frequently encountered in clinical laboratories

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Abstract

The new Vitek 2 GN card (bioMérieux, Marcy-l'Etoile, France) was developed for better identification of fermenting and nonfermenting bacilli. This new card allows the identification of 159 taxa. A total of 426 isolates (331 fermenting and 95 nonfermenting gram-negative bacilli) belonging to 70 taxa covered by the database were evaluated. All isolates were identified in parallel with the ID 32 GN, the API 20E, and the API 20NE methods. The system correctly identified 97.4% (n=415) of the strains. Only 2.1% (n=9) needed additional testing. One strain (0.25%) was misidentified (Klebsiella pneumoniae subsp. pneumoniae), and another one (0.25%) was not identified (Morganella morganii subsp. morganii). The new GN card gives more accurate identifications overall for gram-negative bacilli when compared to the systems described in other similar studies.

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Acknowledgements

We thank Sandrine Pétré, Bernadette Blanc, Béatrice Celliere, Geneviève Bossy, Daniel Monget, and Dave Pincus for their useful discussions about this study.

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Authors and Affiliations

  1. Université Claude Bernard Lyon 1, ISPB, Laboratoire de Microbiologie, EA 3090, 8 av. Rockefeller, 69373, Lyon Cedex 8, France

    F. N. R. Renaud, E. Bergeron & J. Freney

  2. Hôpital de la Croix Rousse, Laboratoire de Microbiologie, 93 rue Croix Rousse, 69004, Lyon, France

    S. Tigaud

  3. Centre Léon Bérard, Laboratoire de Microbiologie, 28 rue Laennec, 69008, Lyon, France

    C. Fuhrmann

  4. Clinique Mutualiste Eugène André, 107 rue Trarieux, 69003, Lyon, France

    B. Gravagna

Authors
  1. F. N. R. Renaud
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  2. E. Bergeron
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  3. S. Tigaud
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  4. C. Fuhrmann
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  5. B. Gravagna
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  6. J. Freney
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Corresponding author

Correspondence to F. N. R. Renaud.

Additional information

This study was presented in part at the 104th General Meeting of the American Society for Microbiology, New Orleans, Louisiana, 2004, Abstract C-180.

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Renaud, F.N.R., Bergeron, E., Tigaud, S. et al. Evaluation of the new Vitek 2 GN card for the identification of gram-negative bacilli frequently encountered in clinical laboratories. Eur J Clin Microbiol Infect Dis 24, 671–676 (2005). https://doi.org/10.1007/s10096-005-0026-6

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  • DOI: https://doi.org/10.1007/s10096-005-0026-6

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