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Interictal levels of calcitonin gene related peptide in gingival crevicular fluid of chronic migraine patients

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Abstract

Calcitonin gene related peptide (CGRP) is a mediator of neurogenic inflammation playing a major role in the pathogenesis of migraine. Increases in serum CGRP have been detected previously in migraineurs and a return to baseline values regarded as successful treatment. As gingival crevicular fluid is known to originate from the serum, the aim of this study is to measure the CGRP content of gingival crevicular fluid (GCF) in chronic migraine patients and to determine whether there is a correlation between serum and GCF values of CGRP. For this study, 24 female individuals suffering from chronic migraine with aura were age-matched with 15 healthy individuals. Serum and GCF samples were obtained from both groups and enzyme linked immunosorbent assay performed to measure CGRP concentration. The level of CGRP in the serum and GCF of chronic migraine patients was 41 ± 16 pg/mL and 0.25 ± 0.09 pg/μg respectively while in healthy individuals CGRP levels were 29 ± 8 pg/mL and 0.19 ± 0.07 pg/μg. The correlation between CGRP levels of the GCF and serum was 0.88 for migraineurs and 0.81 in the controls. Only a weak positive relationship was observed between age and CGRP levels in both groups. CGRP levels were higher in migraineurs compared with controls both in serum and GCF. Furthermore there is a strong correlation between CGRP levels of the serum and GCF. The results of this study suggest that CGRP levels of GCF have potential diagnostic purposes in patients with chronic migraine.

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Funding

The authors of the manuscript would like to thank the vice chancellery of the AJA University of Medical Sciences for the funding of this research (grant number: 2274). We would also like to thank Shiraz University of Medical sciences for their support and facilitation in the process of patient selection and sampling.

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Authors and Affiliations

  1. Department of Periodontology, Dental Faculty - Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Tehran, Iran

    Reza Fekrazad

  2. International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research Network (USERN), Tehran, Tehran, Iran

    Reza Fekrazad

  3. Orthodontic Research Center, Department of Orthodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Fars, Iran

    Ahmadreza Sardarian

  4. Department of Physical Medicine and Rehabilitation, School of Medicine, AJA University of Medical Sciences, Tehran, Tehran, Iran

    Kamran Azma

  5. Department of Biochemistry, School of Medicine, AJA University of Medical Sciences, Tehran, Tehran, Iran

    Masoumeh Karami

  6. Clinical Neurology Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Fars, Iran

    Afshin Borhani-Haghighi

  7. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Fars, Iran

    Behrouz Gharesi-Fard

  8. Department of Biochemistry, School of Medicine, Fasa University of Medical Sciences, Fasa, Fars, Iran

    Bahram Movahedi

Authors
  1. Reza Fekrazad
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  2. Ahmadreza Sardarian
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  3. Kamran Azma
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  4. Masoumeh Karami
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  5. Afshin Borhani-Haghighi
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Correspondence to Ahmadreza Sardarian.

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Fekrazad, R., Sardarian, A., Azma, K. et al. Interictal levels of calcitonin gene related peptide in gingival crevicular fluid of chronic migraine patients. Neurol Sci 39, 1217–1223 (2018). https://doi.org/10.1007/s10072-018-3340-3

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  • DOI: https://doi.org/10.1007/s10072-018-3340-3

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