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Involvement of oxidative stress in SAMP10 mice with age-related neurodegeneration

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Abstract

Age-related changes in the brain tissue are reflected in many aspects. We sought to determine the morphology, Nissl bodies, behavioral appearance and oxidative stress in the brain using SAMP10 mice, a substrain of the senescence-accelerated mouse. SAMP10 mice groups divided by different ages (3, 5, 8 and 14 months) were compared with those of control groups with the above corresponding ages. Cortical thickness, Nissl bodies, behavioral appearance and oxidative stress were evaluated through image software, thionine staining, step-down test and colorimetry, respectively. The weight and cortical thickness of the brain in SAMP10 mice significantly reduced from 8 months of age. The results showed that the number of Nissl bodies decreased or Nissl bodies shrank with dark staining in histology. The same result appeared in a step-down test. As the SAMP10 mice grew older, the oxidative stress-related markers superoxide dismutase decreased and malondialdehyde increased after 8 months. Glutathione peroxidase activities showed no age-related changes. The changes of brain morphology and productions of oxidative stress in the brain tissue might contribute to the behavioral abnormality. Deceleration of age-related production of oxidative stress might be expected to be a potent strategy for anti-aging interventions.

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References

  1. Reed AL, Tanaka A, Sorescu D, Liu H, Jeong EM, Sturdy M, Walp ER, Dudley SC Jr, Sutliff RL (2011) Diastolic dysfunction is associated with cardiac fibrosis in the senescence-accelerated mouse. Am J Physiol Heart Circ Physiol 301:H824–H831

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Atsuyoshi S, Sanae H (2011) Senescence-accelerated mice (SAMs) as a model for brain aging and immunosenescence. Aging Dis 2:414–435

    Google Scholar 

  3. Reuter BK, Pastorelli L, Brogi M, Garg RR, McBride JA, Rowlett RM, Arrieta MC, Wang XM, Keller EJ, Feldman SH, Mize JR, Cominelli F, Meddings JB, Pizarro TT (2011) Spontaneous, immune-mediated gastric inflammation in SAMP1/YitFc mice, a model of Crohn’s-like gastritis. Gastroenterology 141:1709–1719

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Ota H, Akishita M, Akiyoshi T, Kahyo T, Setou M, Ogawa S, Iijima K, Eto M, Ouchi Y (2012) Testosterone deficiency accelerates neuronal and vascular aging of SAMP8 mice: protective role of eNOS and SIRT1. PLoS One 7:e29598

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Sato E, Oda N, Ozaki N, Hashimoto S, Kurokawa T, Ishibashi S (1996) Early and transient increase in oxidative stress in the cerebral cortex of senescence-accelerated mouse. Mech Ageing Dev 86:105–114

    Article  CAS  PubMed  Google Scholar 

  6. Toshio T, Masanori H, Keiichi (1997) Senescence-accelerated mouse (SAM): A novel murine model of senescence. Exp Gerontol 32:105–109

  7. Zussy C, Brureau A, Keller E, Marchal S, Blayo C, Delair B, Ixart G, Maurice T, Givalois L (2013) Alzheimer’s disease related markers, cellular toxicity and behavioral deficits induced six weeks after oligomeric Amyloid-β peptide injection in rats. PLoS One 8:e53117

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Chan KH, Lam KS, Cheng OY, Kwan JS, Ho PW, Cheng KK, Chung SK, Ho JW, Guo VY, Xu A (2012) Adiponectin is protective against oxidative stress induced cytotoxicity in amyloid-beta neurotoxicity. PLoS On 7:e52354

    Article  CAS  Google Scholar 

  9. Quiroz-Baez R, Flores-Domínguez D, Arias C (2013) Synaptic aging is associated with mitochondrial dysfunction, reduced antioxidant contents and increased vulnerability to amyloid-β toxicity. Curr Alzheimer Res 10:324–331

    Article  CAS  PubMed  Google Scholar 

  10. Puca AA, Carrizzo A, Villa F, Ferrario A, Casaburo M, Maciąg A, Vecchione C (2013) Vascular aging: the role of oxidative stress. Int J Biochem Cell Biol 45:556–559

    Article  CAS  PubMed  Google Scholar 

  11. Andrade JP, Assunção M (2012) Protective effects of chronic green tea consumption on age-related neurodegeneration. Curr Pharm Des 18:4–14

    Article  CAS  PubMed  Google Scholar 

  12. Ince I, Kayalar H, Elgin G, Koksal C, Yavasoglu NU (2012) Antioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®. Pharm Biol 50:720–726

    Article  CAS  PubMed  Google Scholar 

  13. Mandawad GG, Dawane BS, Beedkar SD, Khobragade CN, Yemul OS (2013) Trisubstituted thiophene analogues of 1-thiazolyl-2-pyrazoline, super oxidase inhibitors and free radical scavengers. Bioorg Med Chem 21:365–372

    Article  CAS  PubMed  Google Scholar 

  14. Carney JM, Starke-Reed PE, Oliver CN, Landum RW, Cheng MS, Wu JF, Floyd RA (1991) Reversal of age-related increase in brain protein oxidation, decrease in enzyme activity, and loss in temporal and spatial memory by chronic administration of the spin-trapping compound N-tert-butyl-alpha-phenylnitrone. Proc Natl Acad Sci USA 88:3633–3636

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Shimada A, Hosokawa M, Ohta A, Akiguchi I, Takeda T (1994) Localization of atrophy-prone areas in the ageing mouse brain: comparison between the brain atrophy model SAM-P/10 and the normal control SAM-R/1. Neuroscience 59:859–869

    Article  CAS  PubMed  Google Scholar 

  16. Wang Jun, Lei Hongtao, Li Pengtao, Han Li, Hou Jincai, Yan Yangyang, Zhao Haiping, Tsuji Tomoko (2012) The anti-brain ageing effects of krill phosphatidylserine in SAMP10 mice. J Agric Sci 9:196–208

    Google Scholar 

  17. Shuchang H, Qiao N, Piye N, Mingwei H, Xiaoshu S, Feng S, Sheng W, Opler M (2008) Protective effects of gastrodia elata on aluminium-chloride-induced learning impairments and alterations of amino acid neurotransmitter release in adult rats. Restor Neurol Neurosci 26:467–473

    PubMed  PubMed Central  Google Scholar 

  18. Benetti F, Izquierdo I (2013) Histamine infused into basolateral amygdala enhances memory consolidation of inhibitory avoidance. Int J Neuropsychopharmacol 11:1–7

    Google Scholar 

  19. Minami M, Yoshikawa H (1979) A simplified assay method of superoxide dismutase activity for clinical use. Clin Chem Acta 92:337–342

    Article  CAS  Google Scholar 

  20. Zhang H, Jiang Y, He Z, Ma M (2005) Cadmium accumulation and oxidative burst in garlic (Allium sativum). J Plant Physiol 162: 977–984

  21. Lawrence RA, Burk RF (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952–958

    Article  CAS  PubMed  Google Scholar 

  22. Kumagai N, Chiba Y, Hosono M, Fujii M, Kawamura N, Keino H, Yoshikawa K, Ishii S, Saitoh Y, Satoh M, Shimada A, Hosokawa M (2007) Involvement of pro-inflammatory cytokines and microglia in an age-associated neurodegeneration model, the SAMP10 mouse. Brain Res 1185:75–85

    Article  CAS  PubMed  Google Scholar 

  23. Davis AM (1995) The Bcl-2 family of proteins, and the regulation of neuronal survival. Trends Neurosci 18:355–358

    Article  Google Scholar 

  24. Kroemer G (1997) The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nat Med 3:614–619

    Article  CAS  PubMed  Google Scholar 

  25. Numata T, Saito T, Maekawa K, Takahashi Y, Saitoh H, Hosokawa T, Fujita H, Kurasaki M (2002) Bcl-2-linked apoptosis due to increase in NO synthase in brain of SAMP10. Biochem Biophys Res Commun 297:517–522

    Article  CAS  PubMed  Google Scholar 

  26. Shimada A, Keino H, Satoh M, Kishikawa M, Seriu N, Hosokawa M (2002) Age-related progressive neuronal DNA damage associated with cerebral degeneration in a mouse model of accelerated senescence. J Gerontol A Biol Sci Med Sci 57:B415–B421

    Article  PubMed  Google Scholar 

  27. Switzer RC, De Olmos J, Heimer L (1985) Olfactory system. In: Paxinos G (ed) The rat nervous system. Academic Press, Sydney, pp 1–36

    Google Scholar 

  28. Chan KH, Lam KS, Cheng OY, Kwan JS, Ho PW, Cheng KK, Chung SK, Ho JW, Guo VY, Xu A (2012) Adiponectin is protective against oxidative stress induced cytotoxicity in amyloid-beta neurotoxicity. PLoS One 7:e52354

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Grompone G, Martorell P, Llopis S, González N, Genovés S, Mulet AP, Fernández-Calero T, Tiscornia I, Bollati-Fogolín M, Chambaud I, Foligné B, Montserrat A, Ramón D (2012) Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans. PLoS One 7:e52493

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Butterfield DA, Drake J, Pocernich C, Castegna A (2001) Evidence of oxidative damage in Alzheimer’s disease brain: central role for amyloid β-peptide. Trends Mol Med 7:548–554

    Article  CAS  PubMed  Google Scholar 

  31. Coyle JT, Puttfarcken P (1993) Oxidative stress, glutamate, and neurodegenerative disorders. Science 262:689–695

    Article  CAS  PubMed  Google Scholar 

  32. Abdel-Salam OM, Salem NA, El-Shamarka ME, Hussein JS, Ahmed NA, El-Nagar ME (2012) Studies on the effects of aspartame on memory and oxidative stress in brain of mice. Eur Rev Med Pharmacol Sci 16:2092–2101

    CAS  PubMed  Google Scholar 

  33. Wang HM, Wang LW, Liu XM, Li CL, Xu SP, Farooq AD (2013) Neuroprotective effects of forsythiaside on learning and memory deficits in senescence-accelerated mouse prone (SAMP8) mice. Pharmacol Biochem Behav 105:134–141

    Article  CAS  PubMed  Google Scholar 

  34. Jha N, Jurma O, Lalli G, Liu Y, Pettus EH, Greenamyre JT, Liu RM, Forman HJ, Andersen JK (2000) Glutathione depletion in PC12 results in selective inhibition of mitochondrial complex I activity: implications for Parkinson’s disease. J Biol Chem 275:26096–26101

    Article  CAS  PubMed  Google Scholar 

  35. Currais A, Maher P (2013) Functional consequences of age-dependent changes in glutathione status in the brain. Antioxid Redox Signal 9:813–822

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (Grant Nos. 81102679, 81473449), the Fundamental Research Funds for the Central public welfare research institutes (ZZ070824), the National Basic Research Program of China (973 Program, 2015CB554400).

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Authors and Affiliations

  1. Institute of Basic Theory, China Academy of Chinese Medical Sciences, 16 Dong Zhi Men Nei Nan Xiao Jie, Beijing, 100700, China

    Jun Wang

  2. The Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China

    Hongtao Lei

  3. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, 1 Xiyuan Caochang, Hai Dian District, Beijing, 100091, China

    Jincai Hou & Jianxun Liu

  4. Beijing Key Laboratory of Pharmacology of Chinese Materia Medicine, 1 Xiyuan Caochang, Hai Dian District, Beijing, 100091, China

    Jianxun Liu

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  1. Jun Wang
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  2. Hongtao Lei
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Correspondence to Jianxun Liu.

Additional information

J. Wang and H. Lei contributed equally to this paper.

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Wang, J., Lei, H., Hou, J. et al. Involvement of oxidative stress in SAMP10 mice with age-related neurodegeneration. Neurol Sci 36, 743–750 (2015). https://doi.org/10.1007/s10072-014-2029-5

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  • DOI: https://doi.org/10.1007/s10072-014-2029-5

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