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Hesperidin attenuates oxidative and neuronal damage caused by global cerebral ischemia/reperfusion in a C57BL/J6 mouse model

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Abstract

The aim of this study was to determine the effects of hesperidin (HP) on neuronal damage in brain tissue caused by global cerebral ischemia/reperfusion (I/R) in C57BL/J6 mice. For this purpose, a total of 40 mice were divided equally into four groups: (1) sham-operated (SH), (2) global cerebral I/R, (3) HP, and (4) HP+I/R. The SH group was used as a control. In the I/R group, the bilateral carotid arteries were clipped for 15 min, and the mice were treated with vehicle for 10 days. In the HP group, mice were administered HP (100 mg/kg) for 10 days without carotid occlusion. In the HP+I/R group, the I/R model was applied to the mice exactly as in the I/R group, and they were then treated with 100 mg/kg HP for 10 days. Cerebral I/R significantly induced oxidative stress via an increase in lipid peroxidation and a decrease in the components of the antioxidant defense system. Furthermore, cerebral I/R increased the incidence of histopathological damage and apoptosis in brain tissue. HP treatment significantly reversed the oxidative effects of I/R and inhibited the development of neurodegenerative histopathology. Therefore, the current study demonstrates that HP treatment effectively prevents oxidative and histological damage in the brain caused by global I/R. In this context, the beneficial effects of HP are likely a result of its strong antioxidant and free radical-scavenging properties. HP may be an useful treatment to attenuate the negative effects of global cerebral I/R.

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Acknowledgments

We acknowledge the support of IUBAP (Scientific Research Fund of Inonu University) under Grant 2013/205.

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The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Brain and Neurosurgery, Faculty of Medicine, University of Inonu, 44280, Malatya, Turkey

    M. Namik Oztanir & M. Arif Aladag

  2. Department of Pharmacology, Faculty of Medicine, University of Inonu, 44280, Malatya, Turkey

    Osman Ciftci

  3. Department of Histology and Embryology, Faculty Medicine, University of Inonu, 44280, Malatya, Turkey

    Aslı Cetin

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  1. M. Namik Oztanir
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  2. Osman Ciftci
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  3. Aslı Cetin
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  4. M. Arif Aladag
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Correspondence to M. Namik Oztanir.

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Oztanir, M.N., Ciftci, O., Cetin, A. et al. Hesperidin attenuates oxidative and neuronal damage caused by global cerebral ischemia/reperfusion in a C57BL/J6 mouse model. Neurol Sci 35, 1393–1399 (2014). https://doi.org/10.1007/s10072-014-1725-5

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  • DOI: https://doi.org/10.1007/s10072-014-1725-5

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