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Ginsenoside-Rd attenuates TRPM7 and ASIC1a but promotes ASIC2a expression in rats after focal cerebral ischemia

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Abstract

Our previous studies have showed that ginsenoside (GS)-Rd, a mono-compound isolated from traditional Chinese herb panax ginseng, has the neuroprotective effects following ischemic stroke. However, the underlying mechanisms are still largely unknown. Our latest study showed that GS-Rd could block calcium influx in cultured cortical neurons after excitotoxic injury, indicating that GS-Rd may act on cation channels. To explore this possibility, in this study, we used a rat middle cerebral artery occlusion (MCAO) model to examine the effects of GS-Rd on the expression of non-selective cation channels, including transient receptor potential melastatin (TRPM) and acid sensing ion channels (ASIC), and cation channels, including N-methyl-d-aspartate (NMDA) receptors, which all play essential roles in ischemic stroke. Our results showed that both TRPM and ASIC channels were expressed in the brain. At 24 h following MCAO insult, mRNA and protein expression levels of TRPM7, ASIC1a and ASIC2a were significantly increased. Pretreatment of 10 mg/kg GS-Rd attenuated MCAO-induced expression of TRPM7 and ASIC1a but promoted that of ASIC2a. In contrast, GS-Rd had no significant effects on the expression of NMDA receptors. Thus, our results suggest that GS-Rd neuroprotection following cerebral ischemia may be at least due to its effects on the expression of TRPM7, ASIC1a and ASIC2a.

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Acknowledgments

The authors are grateful to Ms. Dongyun Feng for technical assistance. This work was supported by grants from the National Natural Science Foundation of China (No. 31170801).

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The authors declare that they have no conflict of interest.

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  1. Linfu Zhou

    Present address: Department of Neurology, Third Hospital of PLA, Baoji, 721004, China

Authors and Affiliations

  1. Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, China

    Yunxia Zhang, Linfu Zhou, Xiao Zhang, Ming Shi & Gang Zhao

  2. Department of Immunology, Fourth Military Medical University, Xi’an, 710032, China

    Jiuxu Bai

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  1. Yunxia Zhang
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Correspondence to Ming Shi or Gang Zhao.

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Y. Zhang and L. Zhou contributed equally to this work.

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Zhang, Y., Zhou, L., Zhang, X. et al. Ginsenoside-Rd attenuates TRPM7 and ASIC1a but promotes ASIC2a expression in rats after focal cerebral ischemia. Neurol Sci 33, 1125–1131 (2012). https://doi.org/10.1007/s10072-011-0916-6

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