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Protective effects of heme oxygenase-1 against MPP+-induced cytotoxicity in PC-12 cells

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Abstract

Heme oxygenase-1 (HO-1) catalyses the rate-limiting step of heme degradation to biliverdin, which is in turn reduced to bilirubin, CO and free iron. HO-1 can be induced by several harmful stimuli including oxidative stress, and it has a protective role against the cytotoxicity in different cells. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridinium (MPP+) is a neurotoxic substance that induces the degeneration of dopaminergic neurons. This study examined whether HO-1 can be induced by MPP+ and whether HO-1 has a protective role against the MPP+-induced cytotoxicity in PC-12 cells. MPP+ triggered a relatively rapid induction of HO-1. The MPP+-induced cytotoxicity and reactive oxygen species (ROS) production markedly increased by HO-1 inhibitor, zinc protoporphyrin-IX (ZnPP-IX). The increase of ROS production by ZnPP-IX was completely abrogated by either two products of HO (biliverdin or bilirubin) while the increase of cytotoxicity by ZnPP-IX was attenuated partially. These suggest that HO-1 expression might have some cytoprotective effect against MPP+-induced cytotoxicity.

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Authors and Affiliations

  1. Laboratory of Pharmacology, College of Pharmacy, Sungkyunkwan University, Chunchun-dong, Suwon, 440-746, Republic of Korea

    Jung-Woo Bae, Mi-Jeong Kim, Choon-Gon Jang & Seok-Yong Lee

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  1. Jung-Woo Bae
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  2. Mi-Jeong Kim
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Correspondence to Seok-Yong Lee.

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Bae, JW., Kim, MJ., Jang, CG. et al. Protective effects of heme oxygenase-1 against MPP+-induced cytotoxicity in PC-12 cells. Neurol Sci 31, 307–313 (2010). https://doi.org/10.1007/s10072-010-0216-6

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  • DOI: https://doi.org/10.1007/s10072-010-0216-6

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