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Understanding response surface optimization of medium composition for pullulan production from de-oiled rice bran by Aureobasidium pullulans

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Abstract

Central composite rotatable design of RSM was used for the optimization of medium composition for pullulan production from de-oiled rice bran by Aureobasidium pullulans in shake-flask fermentations. The sugars from de-oiled rice bran were extracted in distilled water under moist steam pressure and the obtained de-oiled rice bran extract (DRBE) was used for the optimization of medium composition. RSM optimized medium components (DRBE sugars, 3.88%; yeast extract, 0.24%; (NH4)2SO4, 0.06%; K2HPO4, 0.57% (w/v), and pH, 5.22) supported 5.48% (w/v) pullulan production and 0.88 (A600/100) biomass yield. Coefficient of determination for pullulan production (0.99) and biomass yield (0.99) was close to 1.0 which justifies significance of model. Lack of fit for both responses was non-significant, which shows fitness of quadratic model. FTIR and NMR spectral attributes confirmed the structure of pullulan. XRD patterns verified the amorphous nature of pullulan. De-oiled rice bran was found as a potential substrate for pullulan production.

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Acknowledgement

Authors acknowledge Head, Department of Biotechnology, Punjabi University, Patiala, for providing essential laboratory facilities to execute the present work.

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  1. Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab, 147 002, India

    R. S. Singh & Navpreet Kaur

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  1. R. S. Singh
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  2. Navpreet Kaur
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Singh, R.S., Kaur, N. Understanding response surface optimization of medium composition for pullulan production from de-oiled rice bran by Aureobasidium pullulans. Food Sci Biotechnol 28, 1507–1520 (2019). https://doi.org/10.1007/s10068-019-00585-w

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