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Effect of sodium selenite on the bacteria growth, selenium accumulation, and selenium biotransformation in Pediococcus acidilactici

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Abstract

In this study, the effect of low selenium concentrations on bacteria growth, selenium bioaccumulation, and selenium speciation in Pediococcus acidilactici was investigated. Six different sodium selenite (Na2SeO3) solutions with concentrations of 0, 0.5, 1, 2, 3, and 4 mg/L were added in MRS broth for 24 h. Then, the obtained bacterial pellets were weighed. The contents of total selenium and selenium species in the bacterial pellets were measured via optimized enzymatic hydrolysis and HPLC-ICP-MS. The maximum dried P. acidilactici biomass of 1.44 g/L was achieved by utilizing 1 mg/L Na2SeO3. By increasing sodium selenite concentrations, total selenium contents were significantly increased from 0.14 to 1.45 mg/g dry weight (p < 0.05). The findings indicated that selenium was favorably incorporated into the bacteria protein fraction and mainly formed selenocysteine. Therefore, selenium-enriched lactic acid bacterium P. acidilactici can deliver a less-toxic, more bioavailable selenium source for human and animal nutrition.

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Acknowledgements

This work was financially supported by Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

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Authors and Affiliations

  1. Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Km 9 Darya Boulevard, P.O. Box 578, Sari, Iran

    Masoud Kousha, Sakineh Yeganeh & Abdolsamad Keramat Amirkolaie

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  1. Masoud Kousha
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  2. Sakineh Yeganeh
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  3. Abdolsamad Keramat Amirkolaie
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Correspondence to Sakineh Yeganeh.

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Kousha, M., Yeganeh, S. & Keramat Amirkolaie, A. Effect of sodium selenite on the bacteria growth, selenium accumulation, and selenium biotransformation in Pediococcus acidilactici . Food Sci Biotechnol 26, 1013–1018 (2017). https://doi.org/10.1007/s10068-017-0142-y

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  • DOI: https://doi.org/10.1007/s10068-017-0142-y

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