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Saccharomyces boulardii: Optimization of simultaneous saccharification and fermentation of cell production in organic and conventional apple substrate pulp

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Abstract

A face-centered factorial design was used to study the influence of temperature, cellulase, and pectinase concentration on the production of Saccharomyces boulardii cells during simultaneous saccharification and fermentation of organic and conventional apple substrate pulp. The effects of the variables fermentation temperature (25–35 °C), pectinase concentration (5–25 μL/100 g), and cellulase concentration (4–8 μL/100 g) were analyzed by multiple regression and polynomial models of second order, providing the ideal conditions for yeast cultivation. Cellular production of apple substrates was expressed in log CFU/mL. The optimum condition for temperature was 27.5 °C, and 20 and 5 μL/100 g for pectinase and 8 and 7 μL/100 g for cellulase concentrations for organic and conventional apple pulp, respectively. The observed viability values were in agreement with the predicted values of 8.352 log CFU/mL (organic) and 8.317 log CFU/mL (conventional) apple pulps, thus proving the effectiveness of the models.

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Acknowledgements

This research was developed with the support of the Londrina State University, which provided the infrastructure and facilities. This study was supported by a scholarship from the National Council of Technological and Scientific Development and Coordination for the Improvement of Higher Education Personnel.

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Authors and Affiliations

  1. Department of Food Science and Technology, Center of Agricultural Sciences, Londrina State University, Celso Garcia Cid Highway - PR Route 445, Km 380, P.O. Box 10011, Londrina, Brazil

    Fernanda Silva Farinazzo, Wilma Aparecida Spinosa & Sandra Garcia

  2. School of Medicine, Pontifical Catholic University of Paraná, Jockey Club avenue, 485 - Hipica, Londrina, 86067-000, Brazil

    Eduardo Silva Farinazzo

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  1. Fernanda Silva Farinazzo
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  2. Eduardo Silva Farinazzo
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Correspondence to Fernanda Silva Farinazzo.

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Farinazzo, F.S., Farinazzo, E.S., Spinosa, W.A. et al. Saccharomyces boulardii: Optimization of simultaneous saccharification and fermentation of cell production in organic and conventional apple substrate pulp. Food Sci Biotechnol 26, 969–977 (2017). https://doi.org/10.1007/s10068-017-0123-1

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  • DOI: https://doi.org/10.1007/s10068-017-0123-1

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