Skip to main content
SpringerLink
Log in

Effects of Allium hookeri root water extracts on inhibition of adipogenesis and GLUT-4 expression in 3T3-L1 adipocytes

  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Anti-adipogenic and antidiabetic activities of Allium hookeri root water extracts (ARW) were assessed. Oil Red O staining showed that treatment with ARW caused a dose-dependent reduction in lipid accumulation. ARW was also involved in adipocyte lipolysis via LPL activity, and in the concentration of glycerol in a culture medium. On the basis of the concentration of adipokines following ARW treatment, ARW appeared to inhibit expression of PPAR-γ, to reduce concentrations of leptin and resistin, to increase the concentration of adiponectin, and to inhibit lipid accumulation. ARW modulated adipokine expression associated with insulin resistance and sensitivity. 3T3-L1 adipocytes treated with ARW showed increased GLUT-4 expression with increased glucose uptake into adipocytes. ARW showed effectiveness for improvement of diabetic conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ward WK, Beard JC, Halter JB, Pfeifer MA, Porte DJ. Pathophysiology of insulin secretion in non-insulin-dependent diabetes mellitus. Diabetes Care 7: 491–502 (1984)

    Article  CAS  Google Scholar 

  2. Robertson RP, Harmon J, Tran PO, Tanaka Y, Takahashi H. Glucose toxicity in beta-cells: type 2 diabetes, good radicals gone bad, and the glutathione connection. Diabetes 52: 581–587 (2003)

    Article  CAS  Google Scholar 

  3. Hotamisligil GS, Budavari A, Spiegelman BM. Reduced tyrosine kinase activity of the insulin receptor in obesity-diabetes. Central role of tumor necrosis factor-alpha. J. Clin. Invest. 94: 1543–1549 (1994)

    CAS  Google Scholar 

  4. Hwang EY, Hong JH, Choi JH, Lee EJ, Lee IS. Study on anti-obesity and hypoglycemic effects of Lycium chinense mill extracts. J. Korean Soc. Food Sci. Nutr. 38: 1528–1534 (2009)

    Article  CAS  Google Scholar 

  5. Kim JY, Shin M, Heo YR. Effects of stabilized rice bran on obesity and antioxidative enzyme activity in high fat diet-induced obese C57BL/6 mice. J. Korean Soc. Food Sci. Nutr. 43: 1148–1157 (2014)

    Article  CAS  Google Scholar 

  6. Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Pratley RE, Tataranni PA. Hypoadiponectinemia in obesity and type 2 diabetes: Close association with insulin resistance and hyperinsulinemia. J. Clin. Endocr. Metab. 86: 1930–1935 (2001)

    Article  CAS  Google Scholar 

  7. Sohn JH, Kim TY. Recent update on pathogenesis of nonalcoholic fatty liver disease. Korean J. Med. 79: 461–474 (2010)

    CAS  Google Scholar 

  8. Lee SM, Shin MJ, Seong SI, Hwang KY, Lee JY, Kwon O, Jin, T, Chung JH. 1-Deoxynojirimycin isolated from a Bacillus subtillis stimulates adiponectin and GLUT4 expressions in 3T3-L1 adipocytes. J. Microbiol. Biotechn. 23: 637–643 (2013)

    Article  CAS  Google Scholar 

  9. Green BD, Flatt PR, Bailey CJ. Dipeptidyl peptidase IV (DPP IV) inhibitors: A newly emerging drug class for the treatment of type 2 diabetes. Diabetes Vasc. Dis. Re. 3: 159–165 (2006)

    Article  Google Scholar 

  10. Park JA, Jin KS, Oh YN, Hyun SK, Choi YH, Kwon HJ, Kim BW. Antiadipogenic effect of Vitis amurensis root methanol extract and its solvent fractions in 3T3-L1 preadipocytes. J. Life Sci. 23: 69–78 (2013)

    Article  Google Scholar 

  11. Pushparaj P, Tan CH, Tan BKH. Effects of Averrhoa bilimbi leaf extract on blood glucose and lipids in streptozotocin-diabetic rats. J. Ethnopharmacol. 72: 69–76 (2000)

    Article  CAS  Google Scholar 

  12. Seong S, Evacuasiany E, Widowati W, Fauziah N, Manik VT, Maesaroh M. Inhibitory potential of rambutan seeds extract and fractions on adipogenesis in 3T3-L1 cell line. J. Exp. Integr. Med. 5: 55–60 (2015)

    Article  Google Scholar 

  13. Bae GC, Bae DY. The anti-inflammatory effects of ethanol extract of Allium Hookeri cultivated in South Korea. Korean J. Herbol. 27: 55–61 (2012)

    Article  Google Scholar 

  14. Rhyu DY, Park SH. Characterization of alkyl thiosulfinate in Allium hookeri root using HPLC-ESI-MS. Appl. Biol. Chem. 56: 457–459 (2013)

    CAS  Google Scholar 

  15. Akash MSH, Rehman K, Chen S. Spice plant Allium cepa: Dietary supplement for treatment of type 2 diabetes mellitus. Nutrition 30: 1128–1137 (2014)

    Article  CAS  Google Scholar 

  16. Fleischauer AT, Poole C, Arab L. Garlic consumption and cancer prevention: Meta-analyses of colorectal and stomach cancers. Am. J. Clin. Nutr. 72: 1047–1052 (2000)

    CAS  Google Scholar 

  17. Song EY, Pyun CW, Hong GE, Lim KW, Lee CH. Effect of addition of Allium hookeri on the quality of fermented sausage with meat from sulfur fed pigs during ripening. Korean J. Food Sci. An. 34: 263–272 (2014)

    Article  Google Scholar 

  18. Sung YY, Yoon TS, Kim SJ, Yang WK, Kim HK. Anti-obesity activity of Allium fistulosum L. extract by down-regulation of the expression of lipogenic genes in high-fat diet-induced obese mice. Mol. Med. Rep. 4: 431–435 (2011)

    CAS  Google Scholar 

  19. Ramirez-Zacarías JL, Castro-Muñozledo F, Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97: 493–497 (1992)

    Article  Google Scholar 

  20. Semenkovich C, Wims M, Noe L, Etienne J, Chan L. Insulin regulation of lipoprotein lipase activity in 3T3-Ll adipocytes is mediated at posttranscriptional and posttranslational levels. J. Biol. Chem. 264: 9030–9038 (1989)

    CAS  Google Scholar 

  21. Moreno DA, Ilic N, Poulev A, Brasaemle DL, Fried SK, Raskin I. Inhibitory effects of grape seed extract on lipases. Nutrition 19: 876–879 (2003)

    Article  CAS  Google Scholar 

  22. Park HS, Choi EJ, Lee JH, Kim GH. Evaluation of Allium vegetables for antiadipogenic, anti-cancer, and anti-inflammatory activities in vitro. J. Life Sci. 52: 127–132 (2013)

    Google Scholar 

  23. Tups A, Ellis C, Moar KM, Logie TJ, Adam CL, Mercer JG, Klingenspor M. Photoperiodic regulation of leptin sensitivity in the Siberian hamster, Phodopus sungorus, is reflected in arcuate nucleus SOCS-3 (suppressor of cytokine signaling) gene expression. Endocrinology 145: 1185–1193 (2004)

    Article  CAS  Google Scholar 

  24. Levy JR, Davenport B, Clore JN, Stevens W. Lipid metabolism and resistin gene expression in insulin-resistant Fischer 344 rat. Am. J. Physiol. Endocrinol. Metab. 282: 626–633 (2002)

    Article  Google Scholar 

  25. Matsuzawa Y, Funahashi T, Kihara S, Shimomura I. Adiponectin and metabolic syndrome. Arterioscl. Throm. Vasc. 24: 29–33 (2004)

    Article  CAS  Google Scholar 

  26. Jamurtas AZ, Theocharis V, Koukoulis G, Stakias N, Fatouros IG, Kouretas D, Koutedakis Y. The effects of acute exercise on serum adiponectin and resistin levels and their relation to insulin sensitivity in overweight males. Eur. J. Appl. Physiol. 97: 122–126 (2006)

    Article  CAS  Google Scholar 

  27. Mae T, Kishida H, Nishiyama T, Tsukagawa M, Konishi E, Kuroda M, Mimaki Y, Sashida Y, Takahashi K, Kawada T, Nakagawa K, Kitahara M. A Licorice ethanolic extract with peroxisome proliferator-activated receptor ligandbinding activity affects diabetes in KK-Ay mice, abdominal obesity in dietinduced obese C57BL mice and hypertension in spontaneously hypertensive rats. J. Nutr. 133: 3369–3377 (2003)

    CAS  Google Scholar 

  28. Choi HY, Kim GH. Inhibitory effects of Allium sacculiferum Max. methanol extracts on ROS production and lipid accumulation during differentiation of 3T3-L1 cells. J. Korean Soc. Food Sci. Nutr. 43: 822–828 (2014)

    Google Scholar 

  29. Park JA, Park C, Han MH, Kim BW, Chung YH, Choi YH. Inhibition of adipocyte differentiation and adipogenesis by aged black garlic extracts in 3T3-L1 preadipocytes. J. Life Sci. 21: 720–728 (2011)

    Article  Google Scholar 

  30. Ku SK, Chung IK, Cheon WH, Kim JW. Allium victorialis leaf extract prevents high fat diet induced obesity in mice. J. Vet. Clin. 28: 280–286 (2011)

    Google Scholar 

  31. Xiea W, Zhanga Y, Wanga N, Zhoua H, Dub L, Maa X, Shia X, Cai G. Novel effects of macrostemonoside A, a compound from Allium macrostemon Bung, on hyperglycemia, hyperlipidemia, and visceral obesity in high-fat diet-fed C57BL/6 mice. Eur. J. Pharmacol. 599: 159–165 (2008)

    Article  Google Scholar 

  32. Kim EJ, Lee DH, Kim HJ, Lee SJ, Ban JO, Cho MC, Jeong HS, Yang Y, Hong JT, Yoon DY. Thiacremonone, a sulfur compound isolated from garlic, attenuates lipid accumulation partially mediated via AMPK activation in 3T3-L1 adipocytes. J. Nutr. Biochem. 23: 1552–1558 (2012)

    Article  CAS  Google Scholar 

  33. Kohn AD, Summers SA, Birnbaum MJ, Roth RA. Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation. J. Biol. Chem. 271: 31372–31378 (1996)

    Article  CAS  Google Scholar 

  34. Kadan S, Saad B, Sasson Y, Zaid H. In vitro evaluations of cytotoxicity of eight antidiabetic medicinal plants and their effect on GLUT4 translocation. Evid-Based Compl. Alt. 2013: 1–9 (2013)

    Article  Google Scholar 

  35. Liu CT, Hsu TW, Chen KM, Tan YP, Lii CK, Sheen LY. The antidiabetic effect of garlic oil is associated with ameliorated oxidative stress but not ameliorated level of pro-inflammatory cytokines in skeletal muscle of streptozotocininduced diabetic rats. J. Tradit. Complement. Med. 2: 135–144 (2012)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Imsil Research Institute of Cheese Science, Imsil, Jeonbuk, 55918, Korea

    Hee Sun Yang, Yu Jin Choi, Hee Yeon Jin, Sang Cheon Lee & Chang Ki Huh

Authors
  1. Hee Sun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Jin Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee Yeon Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sang Cheon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chang Ki Huh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chang Ki Huh.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, H., Choi, Y., Jin, H. et al. Effects of Allium hookeri root water extracts on inhibition of adipogenesis and GLUT-4 expression in 3T3-L1 adipocytes. Food Sci Biotechnol 25, 615–621 (2016). https://doi.org/10.1007/s10068-016-0086-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-016-0086-7

Keywords

Search

Navigation