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Antioxidant activity and phenolic content of brick caps mycelium (Naematoloma sublateritium) extracts

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Abstract

A total of 5 subfractions were obtained from methanol extracts of N. sublateritium mycelia via systemic extraction using n-hexane, chloroform, ethyl acetate, n-butanol, and water. The trolox equivalent antioxidant capacity of the chloroform subfraction was 649.40 μmol/g of sample. The highest ferric reducing/antioxidant power (FRAP) assay value was found in the chloroform subfraction (299.24 μmol of FeSO4·7H2O/g). The chloroform and ethyl acetate subfractions exhibited the highest total phenolic compound contents, with ferulic acid equivalents of 31.66 and 48.57 mg/g, respectively. The phenolic acids in the subfractions were further identified using liquid chromatography-tandem mass spectrometry. The 5 phenolic acids of 4-hydroxybenzoic acid, α-resorcylic acid, 4-coumaric acid, salicylic acid, and gentisic acid were found to be the major phenolics in the mushroom.

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References

  1. Chaudiere J, Ferrari-Iliou R. Intracellular antioxidant: from chemical to biochemical mechanisms. Food Chem. Toxicol. 37: 949–962 (1999)

    Article  CAS  Google Scholar 

  2. Decker EA. Phenolics: prooxidants or antioxidants. Nutr. Res. 55: 396–407 (1997)

    CAS  Google Scholar 

  3. Lo KM, Cheung PCK. Antioxidant activity of extracts from the fruiting bodies of Agrocybe aegerita var. alba. Food Chem. 89: 533–539 (2005)

    Article  CAS  Google Scholar 

  4. Lee IK, Kim YS, Jang YW, Jung JY, Yun BS. New antioxidant polyphenols from the medicinal mushroom Inonotus obliquus. Bioorg. Med. Chem. Lett. 17: 6678–6681 (2007)

    Article  CAS  Google Scholar 

  5. Yoo NH, Yoo ID, Kim JW, Yun BS, Ryoo IJ, Yoon ES, Chinh NT, Kim JP. Sterin C, a new antioxidant from the mycelial culture of the mushroom Stereum hirsutum. Agric. Chem. Biotechnol. 48: 28–41 (2005)

    Google Scholar 

  6. Negishi O, Negishi Y, Aoyagi Y, Sugahara T, Ozawa T. Mercaptancapturing properties of mushrooms. J. Agr. Food Chem. 49: 5509–5514 (2001)

    Article  CAS  Google Scholar 

  7. Fatmawati S, Kurashiki K, Takeno S, Kim Y, Shimizu K, Sato M, Imaizumi K, Takahashi K, Kamiya S, Kaneko S, Kondo R. The inhibitory effect on aldose reductase by an extract of Ganoderma lucidum. Phytother. Res. 23: 28–32 (2009)

    Article  Google Scholar 

  8. Godio RP, Fouce R, Gudina EJ, Martin JF. Agrobacterium tumefaciens-mediated transformation of the antitumor clavaric acidproducing basidiomycete Hypholoma sublateritium. Curr. Genet. 46: 287–294 (2004)

    Article  CAS  Google Scholar 

  9. Lee YL, Kim KM, Jeon BH, Choi JW, Choi S. The n-butanol fraction of Naematoloma sublateritium suppresses the inflammatory response through downregulation of NF-κB in human endothelial cells. Int. J. Mol. Med. 29: 801–808 (2012)

    Google Scholar 

  10. Choi S, Jang HJ, Choi JY, Kim MS, Lee YR, Kim HS, Choi SW, Jeon BH, Won SI, Kim TW, Choi JW. Antioxidant and anticancer activity of fractions of the ethanol extract of Naematoloma sublateritium. J. Med. Plants Res. 6: 1344–1352 (2012)

    Google Scholar 

  11. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalies, and weight matrix choice. Nucleic Acids Res. 22: 4673–4680 (1994)

    Article  CAS  Google Scholar 

  12. Correll JC, Klittich CJR, Leslie JF. Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 77: 1640–1646 (1987)

    Article  Google Scholar 

  13. Innis MA, Gelfand DH, Sninsky JJ, White TJ. Amplification and direct sequencing of fungal ribosomal DNA for phylogenetics. pp. 315–322. In: PCR Protocols: a Guide to the Methods and Applications. Academic Press Inc., New York, NY, USA (1990)

    Google Scholar 

  14. Carbone I, Kohn LM. Ribosomal DNA sequence divergence within international transcribed spacer 1 of the Selerotiniaccae. Mycologia 85: 415–427 (1993)

    Article  CAS  Google Scholar 

  15. Kang AS, Kang TS, Cho SM, Yu SH. Studies on submerged culture and mycelial components of Naematoloma sublateritium mycelia. Korean J. Mycology 29: 22–27 (2001)

    Google Scholar 

  16. Gutfinger T. Polyphenols in olive oils. J. Am. Oil Chem. Soc. 58: 966–968 (1981)

    Article  CAS  Google Scholar 

  17. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109–114 (2000)

    Article  CAS  Google Scholar 

  18. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231–1237 (1999)

    Article  CAS  Google Scholar 

  19. Bakar MFA, Mohamed M, Rahmat Am, Fry J. Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chem. 113: 479–483 (2009)

    Article  Google Scholar 

  20. Chung YC, Chen SJ, Hsu CK, Chang CT, Chou ST. Studies on the antioxidative activity of Graptopetalum paraguayense E. Walther. Food Chem. 91: 419–424 (2005)

    Article  CAS  Google Scholar 

  21. Kang HJ, Chawla SP, Jo C, Kwon JH, Byun MW. Studies on the development of functional powder from citrus peel. Bioresource Technol. 97: 614–620 (2006)

    Article  CAS  Google Scholar 

  22. Gursoy N, Sarikurkcu C, Cengiz M, Solak MH. Antioxidant activities, metal contents, total phenolics, and flavonoids of seven Morchella species. Food Chem. Toxicol. 47: 2381–2388 (2009)

    Article  CAS  Google Scholar 

  23. Wong K, Sabaratnam V, Abdullah N, Kuppusamy U, Naidu M. Effects of cultivation techniques and processing on antimicrobial and antioxidant activities of Hericium erinaceus (Bull.:Fr.) Pers. extracts. Food Technol. Biotech. 47: 47–55 (2009)

    CAS  Google Scholar 

  24. Chatchai T, Saranyu K, Khajeelak C, Chantragan S, Rakrudee S. Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lev. Food Chem. 128: 634–639 (2011)

    Article  Google Scholar 

  25. Barros L, Duenas M, Ferreira ICFR, Baptista P, Santos-Buelga C. Phenolic acids determination by HPLC-DAD-ESI/MS in sixteen different Portuguese wild mushrooms species. Food Chem. Toxicol. 47: 1076–1079 (2009)

    Article  CAS  Google Scholar 

  26. Peungvicha P, Temsiririrkkul R, Prasain JK, Tezuka Y, Kadota S, Thirawarapan SS, Watanabe H. 4-Hydroxybenzoic acid: A hypoglycemic constituent of aqueous extract of Pandanus odorus root. J. Ethnopharmacol. 62: 79–84 (1998)

    Article  CAS  Google Scholar 

  27. Ashidate K, Kawamura M, Mimura D, Tohda H, Miyazaki S, Teramoto T, Yamamoto Y, Hirata Y. Gentisic acid, an aspirin metabolite, inhibits oxidation of low-density lipoprotein and the formation of cholesterol ester hydroperoxides in human plasma. Eur. J. Pharmacol. 513: 173–179 (2005)

    Article  CAS  Google Scholar 

  28. Lodovici M, Raimondi L, Guglielmi F, Gemignani S, Dolara P. Protection against ultraviolet B-induced oxidative DNA damage in rabbit corneal-derived cells (SIRC) by 4-coumaric acid. Toxicology 184: 141–147 (2003)

    Article  CAS  Google Scholar 

  29. Castilho PC, Savluchinske-Feio S, Weinhold TS, Gouveia SC. Evaluation of the antimicrobial and antioxidant activities of essential oils, extracts and their main components from oregano from Madeira Island, Portugal. Food Control 23: 552–558 (2012)

    Article  CAS  Google Scholar 

  30. Heleno SA, Barros L, Sousa MJ, Martins A, Santos-Buelga C, Ferreira ICFR. Targeted metabolites analysis in wild Boletus species. LWT-Food Sci. Technol. 44: 1343–1348 (2011)

    Article  CAS  Google Scholar 

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Li, H., Nam, WS., Moon, B. et al. Antioxidant activity and phenolic content of brick caps mycelium (Naematoloma sublateritium) extracts. Food Sci Biotechnol 23, 1425–1431 (2014). https://doi.org/10.1007/s10068-014-0195-0

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