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Phenylpropanoids from red kohlrabi sprouts inhibits nitric oxide production in RAW 264.7 macrophage cells

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Abstract

The sprouts of red kohlrabi (Brassica oleracea var. gongylodes) were extracted with methanol, and the concentrated extracts were successively partitioned with ethyl acetate, n-butyl alcohol (n-BuOH), and H2O. From the n-BuOH fraction, four phenylpropanoids were isolated through repeated silica gel and ODS column chromatographies. On the basis of physico-chemical and spectroscopic data including NMR, MS, and IR, the isolated compounds were identified as 3-(3,4,5-trimethoxyphenyl)-2E-propenoic acid methyl ester (1), (E)-sinapic acid methyl ester (2), (E)-sinapoyl glucoside (3), and lawsoniaside B (4). Moreover, the compounds dose-dependently inhibited NO production in RAW 264.7 cells with IC50 values of 41.22 (1), 38.32 (3), and 22.65 (4) μM, respectively. With further studies, these compounds may be potentially useful in developing new anti-inflammatory agents.

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Authors and Affiliations

  1. Department of Horticultural Biotechnology, Kyung Hee University, Yongin, Gyeonggi, 446-701, Korea

    Jae-Woong Lee, Dong-Ryeol Baek & Youn-Hyung Lee

  2. Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk, 369-873, Korea

    Dae-Young Lee & Geum-Soog Kim

  3. Department of Oriental Medicinal Materials & Processing, Kyung Hee University, Yongin, Gyeonggi, 446-701, Korea

    Rak-Hoon Jeong & Nam-In Baek

  4. Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Dong-Sung Lee & Youn-Chul Kim

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  1. Jae-Woong Lee
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  2. Dae-Young Lee
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  3. Dong-Ryeol Baek
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  4. Rak-Hoon Jeong
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  9. Youn-Hyung Lee
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Correspondence to Youn-Hyung Lee.

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Lee, JW., Lee, DY., Baek, DR. et al. Phenylpropanoids from red kohlrabi sprouts inhibits nitric oxide production in RAW 264.7 macrophage cells. Food Sci Biotechnol 23, 965–969 (2014). https://doi.org/10.1007/s10068-014-0130-4

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  • DOI: https://doi.org/10.1007/s10068-014-0130-4

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