Skip to main content
Log in

Extraction, multi-response analysis, and optimization of biologically active phenolic compounds from the pulp of Indian jamun fruit

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

An aqueous extraction technique was applied to optimize the extraction process variables of temperature (40°C–60°C), time (20–100 min), and the solid-to-liquid ratio (1:10–1:15) using a Box-Behnken experimental design and response surface methodology. Maximum extraction yields of total phenolics and flavonoids, and anti-oxidant activities were obtained from Indian jamun fruit. Effects of the extraction temperature and the solid-toliquid ratio were found to be significant (p<0.05) for all responses. Second order polynomial models were developed from experimental data to predict the effects of the independent variables on the responses. Optimum extraction conditions (temperature of 54°C, time of 50 min, and a solid-to-liquid ratio of 1:8.5) for the maximum extraction yield of total phenolics (1,332.36 mg GAE/100 g) and flavonoids (110.94 mg QE/100 g), and the anti-oxidant activity (233.55mg AAE/100 g) were achieved. Experimental values matched well with predicted values obtained under optimum conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Duthie GG, Duthie SJ, Kyle JAM. Plant polyphenols in cancer and heart disease: Implications as nutritional antioxidants. Nutr. Res. Rev. 13: 79–106 (2000)

    Article  CAS  Google Scholar 

  2. John JA, Shahidi F. Phenolic compounds and antioxidant activity of Brazil nut (Bertholletia excelsa). J. Funct. Food 2(3): 196–209 (2010)

    Article  CAS  Google Scholar 

  3. Larson RA. The antioxidants of higher plants. Phytochemistry 27: 969–978 (1998)

    Article  Google Scholar 

  4. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J. Agr. Food Chem. 49: 5165–5170 (2001)

    Article  CAS  Google Scholar 

  5. Cai YZ, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J. Agr. Food Chem. 51: 2288–2294 (2003)

    Article  CAS  Google Scholar 

  6. Pokorný J. Are natural antioxidants better -and safer-than synthetic antioxidants? Eur. J. Lipid. Sci. Tech. 109: 629–642 (2007)

    Article  Google Scholar 

  7. Prakash Maran J, Manikandan S. Response surface modeling and optimization of process parameters for aqueous extraction of pigments from prickly pear (opuntia ficus-indica) fruit. Dyes. Pigm. 95: 465–472 (2012)

    Article  CAS  Google Scholar 

  8. Fan G, Han Y, Gu Z, Chen D. Optimizing conditions for anthocyanin extractions from purple sweet potato using response surface methodology. LWT-Food Sci. Technol. 41: 155–160 (2008)

    Article  CAS  Google Scholar 

  9. Bujiæ-Kojiæ A, Planinic M, Srécko T, Jakabek L, Seruga M. Influence of solvent and temperature on extraction of phenolic compounds from grape seed, antioxidant activity and colour of extract. Int. J. Food Sci. Tech. 44: 2394–2401 (2009)

    Article  Google Scholar 

  10. Pinelo M, Rubilar M, Sineiro J, Nunez MJ. Effect of solvent, temperature, and solvent-to-solid ration the total phenolic content and antiradical activity of extracts from different components from grape pomace. J. Agr. Food Chem. 53: 2111–2117 (2005)

    Article  CAS  Google Scholar 

  11. Santos DT, Veggi PC, Meireles AMA. Extraction of antioxidant compounds from jabuticaba (Myrciaria Cauliflora) skins. Yield, composition and economical evaluation. J. Food Eng. 101: 23–31 (2010)

    Article  CAS  Google Scholar 

  12. Thirugnanasambandham K, Sivakumar V, Maran JP. Treatment of egg processing industry effluent using chitosan as an adsorbent. J. Serb. Chem. Soc. DOI: 10.2298/JSC130201053T (2013)

    Google Scholar 

  13. Prakash Maran J, Manikandan S, Vigna Nivetha C, Dinesh R. Ultrasound assisted extraction of bioactive compounds from Nephelium lappaceum L. fruit peel using central composite face centered response surface design. Arab. J. Chem. DOI: 10.1016/j.arabjc.2013.02.007 (2013)

    Google Scholar 

  14. Prakash Maran J, Manikandan S, Priya B, Gurumoorthi P. Box-Behnken design based multi-response analysis and optimization of supercritical carbon dioxide extraction of bioactive flavonoid compounds from tea (Camellia sinensis L.) leaves. J. Food Sci. Technol. DOI: 10.1007/s13197-013-0985-z (2013)

    Google Scholar 

  15. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal. Biochem. 269: 337–341 (1999)

    Article  CAS  Google Scholar 

  16. Prakash Maran J, Sivakumar V, Thirgananasambandham K, Sridhar R. Response surface modeling and analysis of barrier and optical properties of maize starch based edible films. Int. J. Biol. Macromol. 60: 412–421 (2013)

    Article  CAS  Google Scholar 

  17. Prakash Maran P, Sivakumar V, Thirgananasambandham K, Sridhar R. Model development and process optimization for solvent extraction of polyphenols from red grapes using Box-Behnken design. Prep. Biochem. Biotech. 44: 56–67 (2014)

    Article  Google Scholar 

  18. Prakash Maran J, Sivakumar V, Thirgananasambandham K, Kandasamy S. Modelling and analysis of film composition on mechnical properties of maize starch based edible films. Int. J. Biol. Macromol. DOI: 10.1016/j.ijbiomac.2013.09.027 (2013)

    Google Scholar 

  19. Prakash Maran J, Sivakumar V, Thirgananasambandham K, Sridhar R. Degradation behavior of biocomposites based on cassava starch buried under indoor soil conditions. Carbohyd. Polym. 101: 20–28 (2014)

    Article  Google Scholar 

  20. Thirugnanasambandham K, Sivakumar V, Maran JP. Application of chitosan as an adsorbent to treat rice mill wastewater — mechanism, modeling and optimization. Carbohyd. Polym. 97: 451–457 (2013)

    Article  CAS  Google Scholar 

  21. Gan CY, Latiff AA. Optimisation of the solvent extraction of bioactive compounds from Parkia speciosa pod using response surface methodology. Food Chem. 124: 1277–1283 (2011)

    Article  CAS  Google Scholar 

  22. Pompeu DR, Silva EM, Rogez H. Optimisation of the solvent extraction of phenolic antioxidants from fruits of Euterpe oleracea using response surface methodology. Bioresour. Technol. 100: 6067–6082 (2009)

    Article  Google Scholar 

  23. Chirinos R, Rogez H, Campos D, Pedreschi R, Larondelle Y. Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers. Sep. Purif. Technol. 55: 217–225 (2007

    Article  CAS  Google Scholar 

  24. Gong Y, Hou Z, Gao Y, Xue Y, Liu X, Li G. Optimization of extraction parameters of bioactive components from defatted marigold (Tagetes erecta L.) residue using response surface methodology. Food Bioprod. Process. 90: 9–16 (2012)

    Article  CAS  Google Scholar 

  25. Æetkoviæ GS, Djilas SM, Èanadanoviæ-Brunet JM, Tumbas VT. Antioxidant properties of marigold extracts. Food Res. Int. 37: 643–650 (2004)

    Article  Google Scholar 

  26. Li W, Gao Y, Zhao J, Wang Q. Phenolic, flavonoid, and lutein ester content and antioxidant activity of 11 cultivars of Chinese marigold. J. Agr. Food Chem. 55: 8478–8484 (2007)

    Article  CAS  Google Scholar 

  27. Prakash Maran J, Sivakumar V, Thirgananasambandham K, Sridhar R. Optimization of microwave assisted extraction of pectin from orange peel. Carbohyd. Polym. 97: 703–709 (2013)

    Article  CAS  Google Scholar 

  28. Prakash Maran J, Manikandan S, Mekala V. Modeling and optimization of betalain extraction from Opuntia ficus-indica using Box-Behnken design with desirability function. Ind. Crop. Prod. 49: 304–311 (2013)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to V. Sivakumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prakash Maran, J., Sivakumar, V., Thirugnanasambandham, K. et al. Extraction, multi-response analysis, and optimization of biologically active phenolic compounds from the pulp of Indian jamun fruit. Food Sci Biotechnol 23, 9–14 (2014). https://doi.org/10.1007/s10068-014-0002-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-014-0002-y

Keywords

Navigation