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Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells

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Abstract

Nepalese sumac (Rhus parviflora) is a wild edible fruit used for the treatment of various ailments including neurological complications and stomach disorders in the traditional medicinal system of south Asia (Ayurveda). Four flavonoids were isolated from ethyl acetate (EtOAc) fraction of Nepalese sumac fruits and their chemical structures were determined on the basis of NMR, fast atom bombardment mass spectrometry (FAB/MS), and IR. The efficiency of isolated compounds in attenuating glutamateinduced cell death in an immortalized mouse hippocampal cell line (HT-22) and inhibition of cycline dependent kinase 5 (Cdk5) were investigated. Among the compounds, flavanols, fustin (1) and taxifolin (2), an aurone, aureusidin (3), and a biflavonoid, agathisflavone (4) were found to have protective effect against glutamate induced oxidative injury in HT22 cells. Aureusidin (3), a Cdk5/p25 inhibitor (IC50 3.5 μM), was the most potent neuroprotector with an EC50 value of 11.90 μM.

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Authors and Affiliations

  1. Graduate School of Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University, Yongin, Gyeonggi, 446-701, Korea

    Sabina Shrestha, Ji-Hae Park, Jin-Gyeong Cho & Nam-In Baek

  2. Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Chungbuk, 369-873, Korea

    Dae-Young Lee

  3. Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Dong-Sung Lee, Bin Li & Youn-Chul Kim

  4. Ildong Research Laboratories, Ildong Pharmaceutical Co., Ltd., Yongin, Gyeonggi, 445-170, Korea

    Yong-Jin Jeon & Seung-Woo Yeon

Authors
  1. Sabina Shrestha
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  2. Dae-Young Lee
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  3. Ji-Hae Park
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  4. Jin-Gyeong Cho
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  5. Dong-Sung Lee
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  6. Bin Li
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  7. Youn-Chul Kim
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  8. Yong-Jin Jeon
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  9. Seung-Woo Yeon
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  10. Nam-In Baek
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Correspondence to Nam-In Baek.

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Shrestha, S., Lee, DY., Park, JH. et al. Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells. Food Sci Biotechnol 22, 895–902 (2013). https://doi.org/10.1007/s10068-013-0161-2

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  • DOI: https://doi.org/10.1007/s10068-013-0161-2

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