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Aqueous two-phase extraction of bromelain from pineapple peels (‘Phu Lae’ cultv.) and its biochemical properties

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Abstract

This study examines the extraction of bromelain from pineapple peels by using an aqueous two-phase system (ATPS). Bromelain from the crude extract predominantly partitioned to the polyethylene glycol rich phase. The best condition for bromelain partitioning was found to be 18% PEG6000-17% MgSO4, which increased purity 3.44-fold with an activity recovery of 206%. Protein patterns and activity staining showed the molecular weight of bromelain to be about 29 kDa. The bromelain showed the highest relative activity at pH 8.0 and at 60°C. When increasing NaCl concentrations (up to 1.5%, w/v), its activity continuously decreased. The bromelain (0–0.3 units) was applied to hydrolyze collagen. The β, α1, α2 components of collagen extensively degraded into small peptides when treated with the bromelain. This study showed that the ATPS can be employed to isolate the bromelain from pineapple peels and the bromelain extract could be used as a meat tenderizing agent.

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Authors and Affiliations

  1. Food Technology Program, School of Agro-Industry, Mae Fah Luang University, Muang, Chiang Rai, 57100, Thailand

    Sunantha Ketnawa & Saroat Rawdkuen

  2. School of Cosmetic Science, Mae Fah Luang University, Muang, Chiang Rai, 57100, Thailand

    Phanuphong Chaiwut

Authors
  1. Sunantha Ketnawa
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  2. Phanuphong Chaiwut
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  3. Saroat Rawdkuen
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Correspondence to Saroat Rawdkuen.

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Ketnawa, S., Chaiwut, P. & Rawdkuen, S. Aqueous two-phase extraction of bromelain from pineapple peels (‘Phu Lae’ cultv.) and its biochemical properties. Food Sci Biotechnol 20, 1219 (2011). https://doi.org/10.1007/s10068-011-0168-5

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  • DOI: https://doi.org/10.1007/s10068-011-0168-5

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