Skip to main content
SpringerLink
Log in

Simultaneous determination of macrolide residues in fish and shrimp by liquid chromatography-tandem mass spectrometry

  • Research Note
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

A sensitive and selective analytical method for 7 macrolides determination by LC-tandem mass spectrometry (LC-MS/MS) in fish and shrimp was developed. Samples were extracted by liquid-liquid extraction steps using 70% acetonitrile. The positive ionization produced the molecular related ions, (M+2H)2+, at m/z 422.3 for spiramycin I, and (M+H)+, at m/z 734.5, 772.4, 828.4, 837.4, 916.5, and 814.4 for erythromycin A, kitasamycin, josamycin, roxithromycin, tylosin A, and oleandomycin, respectively, were the precursor ions for collision-induced dissociation (CID) and 2 diagnostic product ions for each analyte were identified to carry out selected reaction monitoring (SRM) LC-MS/MS analyses. The recovery rates of macrolides in fish and shrimp samples were in the range 68.2–89.0, 72.8–91.6% and 75.2–87.0, 64.4–83.2% for the concentration of 50 and 100 ng/g, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chan W, Gerhardt GC, Salisbury CDC. Determination of tylosin and tilmicosin residues in animal tissues by reversed-phase liquid chromatography. J. AOAC Int. 77: 331–333 (1994)

    CAS  Google Scholar 

  2. Cherlet M, de Baere S, Croubels S, de Backer P. Quantitation of tylosin in swine tissues by liquid chromatography combined with electrospray ionization mass spectrometry. Anal. Chim. Acta 473: 167–175 (2002)

    Article  CAS  Google Scholar 

  3. Hong SS. Lee’s Pharmacology Lecture. Vol. II. Sunil Munhwa Press, Seoul, Korea. pp. 524–556 (1987)

    Google Scholar 

  4. Treves-Brown KM. Applied Fish Pharmacology. pp. 87–94. In: Macrolides. Kluwer Academic Publishers Press, Lodon, UK (2000)

    Google Scholar 

  5. AOAC. Official Method of Analysis of AOAC Intl. 17th ed. Method 988.08. Association of Official Analytical Chemists, Arlington, VA, USA (2000)

    Google Scholar 

  6. Commission of the European Communities. Council regulation No. 2377/90. List of pharmacologically active substances for which maximum residue limits have been fixed. Annex I, Off. J. Eur. Communities. Legis. L224 (1990)

  7. KFDA. Monitoring of antimicrobial resistance on the food-animals and meats. Korea Food & Drug Admistration report, Seoul, Korea. pp. 22–37 (2007)

  8. Korea Animal Health Products Association (KAHPA). The Guide Book of Veterinary Antibiotics. Kyungsungmunhwa Press, Seoul, Korea. pp. 898–923 (2001)

    Google Scholar 

  9. KFDA. Korea Food Code. No. 201072-15. Korea Food & Drug Adminstration, Cheongwon, Korea (2010)

    Google Scholar 

  10. Smither R. Bacterial inhibitors formed during the adventitious growth of microorganisms in chicken liver and pig kidney. J. Appl. Microbiol. 45: 267–277 (1978)

    Article  CAS  Google Scholar 

  11. Kanfer I, Skinner MF, Walker RB. Analysis of macrolide antibiotics. J. Chromatogr. A 812: 255–286 (1998)

    Article  CAS  Google Scholar 

  12. Lingerfelt B, Champney WS. Macrolide and ketolide antibiotic separation by reversed phase high performance liquid chromatography. J. Pharmaceut. Biomed. 20: 459–469 (1999)

    Article  CAS  Google Scholar 

  13. Leal C, Codony R, Compañó R, Granados M, Dolors Prat M. Determination of macrolide antibiotics by liquid chromatography. J. Chromatogr. A 910: 285–290 (2001)

    Article  CAS  Google Scholar 

  14. Horie M, Saito K, Ishii R, Yoshida T, Haramaki, Nakazawa H. Simultaneous determination of five macrolide antibiotics in meat by high-performance liquid chromatography. J. Chromatogr. A 812: 295–302 (1998)

    Article  Google Scholar 

  15. Delepine B, Hurtaud-Pessel D, Sanders P. Multiresidue method for confirmation of macrolide antibiotics in bovine muscle by liquid chromatography/mass spectrometry. J. AOAC Int. 79: 397–404 (1996)

    CAS  Google Scholar 

  16. Draisci R, Palleschi L, Ferretti E, Achene L, Cecilia A. Confirmatory method for macrolide residues in bovine tissues by micro-liquid chromatography-tandem mass spectrometry. J. Chromatogr. A 926: 97–104 (2001)

    Article  CAS  Google Scholar 

  17. Deubel A, Fandiño AS, Sörgel F, Holzgrabe U. Determination of erythromycin and related substances in commercial samples using liquid chromatography/ion trap mass spectrometry. J. Chromatogr. A 1136: 39–47 (2006)

    Article  CAS  Google Scholar 

  18. Horie M, Takegami H, Toya K, Nakazawa H. Determination of macrolide antibiotics in meat and fish by liquid chromatographyelectrospray mass spectrometry. Anal. Chim. Acta 492: 187–197 (2003)

    Article  CAS  Google Scholar 

  19. Labour and Welfare. Analytical methods for residual compositional substances of agricultural chemicals, feed additives, and veterinary drugs in food. Report. No. 0124001. Department of Food Safety Ministry of Health, Labour and Welfare, Syoku-An, Japan. pp. 43–52 (2006)

    Google Scholar 

  20. Causon R. Validation of chromatographic methods in biomedical analysis viewpoint and discussion. J. Chromatogr. B 689: 175–180 (1997)

    Article  CAS  Google Scholar 

  21. Zhang S, Liu Z, Guo X, Cheng L, Wang Z, Shen J. Simultaneous determination and confirmation of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in chicken muscle by liquid chromatography-tandem mass spectrometry. J. Chromatogr. B 875: 399–404 (2008)

    Article  CAS  Google Scholar 

  22. Berrada H, Borrull F, Font G, Marcé RM. Determination of macrolide antibiotics in meat and fish using pressurized liquid extraction and liquid chromatography-mass spectrometry. J. Chromatogr. A 1208: 83–89 (2008)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Aquaculture Industry Division, Southwest Sea Fisheries Research Institute, Yeosu, Jeonnam, 556-823, Korea

    Mi Ra Jo, Tae Seek Lee & Kunbawui Park

  2. Southeast Sea Fisheries Research Institute, National Fisheries Research & Development Institute, Tongyeong, Gyeongnam, 650-943, Korea

    Hee Jung Lee & Poong Ho Kim

  3. Food Safety Division, National Fisheries Research & Development Institute, Busan, 619-705, Korea

    Eun Gyoung Oh & Doo Seog Lee

  4. Department of Food Science, Otsuma Women’s University, Tokyo, 102-8357, Japan

    Masakazu Horie

Authors
  1. Mi Ra Jo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hee Jung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tae Seek Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kunbawui Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eun Gyoung Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Poong Ho Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Doo Seog Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Masakazu Horie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mi Ra Jo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jo, M.R., Lee, H.J., Lee, T.S. et al. Simultaneous determination of macrolide residues in fish and shrimp by liquid chromatography-tandem mass spectrometry. Food Sci Biotechnol 20, 823–827 (2011). https://doi.org/10.1007/s10068-011-0114-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-011-0114-6

Keywords

Search

Navigation