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Rapid method for determination of β-carotene in foods using ultra high performance liquid chromatography

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Abstract

The ultra high performance liquid chromatography (u-HPLC) method for determination of β-carotene in foods was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on a reversed column C18 (particle size 2 μm, i.d. 2 mm, length 50 mm), followed by ultra violet (UV) detection at 450 nm. The recovery of β-carotene was more than 84.4% and the limit of detection and limit of quantitation of u-HPLC analysis were 0.28 and 0.85 μg/mL for β-carotene with butylated hydroxytoluene (BHT) and 0.62 and 1.89 μg/mL for β-carotene without BHT, respectively. The calibration graph for β-carotene was linear from 0.1 to 25.0 μg/mL for u-HPLC. The intra- and interday precisions (relative standard deviations) were <7.5 and <7.8%, respectively. Benefits of u-HPLC analysis of β-carotene in foods is reduction of the analysis time to approximately 1/4, saving the volume of solvent to approximately 1/15. It seems that u-HPLC can offer significant improvements in speed, sensitivity, and resolution compared with conventional HPLC, this bodes well for future applications.

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Authors and Affiliations

  1. Food Analysis Center, Korea Food Research Institute, Seongnam, Gyeonggi, 463-746, Korea

    Jaeho Ha, You-Shin Shim, Hye-Young Seo & Hyun-Jin Nam

  2. Hiroaki Nakagawa Naka Division, Hitachi High-Technologies Corporation, Hitachinaka-shi, Ibaraki-ken, 312-8504, Japan

    Masahito Ito & Hiroaki Nakagawa

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  1. Jaeho Ha
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  2. You-Shin Shim
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  3. Hye-Young Seo
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  4. Hyun-Jin Nam
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  5. Masahito Ito
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  6. Hiroaki Nakagawa
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Correspondence to Jaeho Ha.

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Ha, J., Shim, YS., Seo, HY. et al. Rapid method for determination of β-carotene in foods using ultra high performance liquid chromatography. Food Sci Biotechnol 19, 1199–1204 (2010). https://doi.org/10.1007/s10068-010-0171-2

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  • DOI: https://doi.org/10.1007/s10068-010-0171-2

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