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Antioxidant and antiproliferative activities of grape seeds from different cultivars

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Abstract

Grape seeds contain high levels of phytochemicals, which have been correlated with a decreased risk of chronic diseases. In this study, grape seeds from 10 different cultivars (‘SV 18315’, ‘Naples’, ‘Agawam’, ‘Aligote’, ‘V.50151’, ‘Cabernet Sauvignon’, ‘Urbana’, ‘Baco22A’, ‘Clinton’, and ‘Campbell Early’) were evaluated for their antioxidant and antiproliferative activities. The antioxidant activity of the grape seeds was evaluated by radical scavenging activities and reducing power. The antiproliferative activity was assessed by the inhibition of MCF7, NCI-H460, HCT116, and MKN45 cancer cell proliferation. The total polyphenol and flavonoid contents were determined by spectrophotometric methods. ‘Cabernet Sauvignon’ and ‘Baco22A’ had high polyphenol (4,369.2 and 3,747.0 mg/100 g seeds) and flavonoid contents (3,072.7 and 2,032.9 mg/100 g seeds). ‘Cabernet Sauvignon’ and ‘Baco22A’ also possessed the highest radical scavenging activity. In addition, these were showed generally higher antiproliferative activity than other cultivars. Both the polyphenol and flavonoid contents were positively correlated with radical scavenging activity (R2>0.9). These results may provide basic information about health-beneficial effects of grape seeds.

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References

  1. Squadriato GL, Pryor WA. Oxidative chemistry of nitric oxide: The roles of superoxide, peroxynitrite, and carbon dioxide. Free Radical Bio. Med. 25: 392–403 (1998)

    Article  Google Scholar 

  2. Waris G, Ahsan H. Reactive oxygen species: Role in the development of cancer and various chronic conditions. J. Carcinog. 5: 1–8 (2006)

    Article  Google Scholar 

  3. Moskovitz J, Yim MB, Chock B. Free radicals and disease. Arch. Biochem. Biophys. 2: 354–359 (2002)

    Article  Google Scholar 

  4. Hudson EA, Dinh PA, Kokubu T, Simmonds MSJ, Gescher A. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidem. Biomar. 9: 1163–1170 (2000)

    CAS  Google Scholar 

  5. Hollman PCH. Evidence for health benefits of plant phenols: Local or systemic effects? J. Sci. Food Agr. 81: 842–852 (2001)

    Article  CAS  Google Scholar 

  6. Bartolome B, Hernandez T, Bengoechea ML, Quesada C, Comez-Cordoves C, Estrella I. Determination of some structural features of procyanidins and related compounds by photodiode-array detection. J. Chromatogr. A 723: 19–26 (1996)

    Article  CAS  Google Scholar 

  7. Maier T, Schieber A, Kammerer DR, Carle R. Residues of grpae (Vitis vinifera L.) seed oil production as a valuable source of phenolic antioxidants. Food Chem. 112: 551–559 (2009)

    Article  CAS  Google Scholar 

  8. Bouhamidi B, Prévost V, Nouvelot A. High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated fatty acid against UV-C induced peroxidation. Life Sci. 321: 31–38 (1998)

    CAS  Google Scholar 

  9. Yilmaz Y, Toledo RT. Health aspects of functional grape seed constituents. Food Sci. Biotechnol. 15: 422–433 (2004)

    CAS  Google Scholar 

  10. Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agr. Food Chem. 50: 3010–3014 (2002)

    Article  CAS  Google Scholar 

  11. Choi Y, Gu J, Kim M, Lee J. Antioxidant and antiproliferative activities of methanolic extracts from thirty Korean medicinal plants. Food Sci. Biotechnol. 17: 1235–1239 (2008)

    Google Scholar 

  12. Choi Y, Lee J. Antioxidant and antiproliferative properties of a tocotrienol-rich fraction from grape seeds. Food Chem. 114: 1386–1390 (2009)

    Article  CAS  Google Scholar 

  13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231–1237 (1999)

    Article  CAS  Google Scholar 

  14. Choi Y, Jeong HS, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem. 103: 130–138 (2007)

    Article  CAS  Google Scholar 

  15. Mosmann T. Rapid colormetric assay for celluar growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55–63 (1983)

    Article  CAS  Google Scholar 

  16. Tsao R, Yang R. Optimization of a new mobile phase to know the complex and real polyphenolic composition: Towards a total phenolic index using high performance liquid chromatography. J. Chromatogr. A 1018: 29–40 (2003)

    Article  CAS  Google Scholar 

  17. Baydar NG, Özkan G, Saðiç O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 15: 335–339 (2004)

    Article  Google Scholar 

  18. Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutat. Res. 579: 200–213 (2005)

    CAS  Google Scholar 

  19. Castillo J, Benavente-Garca O, Lorente J, Alcaraz M, Redondo A, Ortuo A, Del Rio JA. Antioxidant activity and radioprotective effects against chromosomal damage induced in vivo by X-rays of flavan-3-ols (procyanidins) from frape seeds (Vitis vinifera): Comparative study versus other phenolic and organic compounds. J. Agr. Food Chem. 48: 1738–1745 (2000)

    Article  CAS  Google Scholar 

  20. Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agr. Food Chem. 43: 1813–1815 (1995)

    Article  CAS  Google Scholar 

  21. Jayaprakisha GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 73: 285–290 (2001)

    Article  Google Scholar 

  22. Velioglu YS, Mazza G, Gao L, Oomah BO. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agr. Food Chem. 46: 4113–4117 (1998)

    Article  CAS  Google Scholar 

  23. Bakkalbasi E, Yemis O, Aslanova D. Major flavan-3-ol composition and antioxidant activity of seeds from different grape varieties grown in Turkey. Eur. Food Res. Technol. 221: 792–797 (2005)

    Article  CAS  Google Scholar 

  24. Guendez R, Kallithraka S, Makris DP, Kefalas P. Determination of low molecular weight polyphenolic constituents in grape (Vitis vinifera sp.) seed extracts: Correlation with antiradical activity. Food Chem. 89: 1–9 (2005)

    Article  CAS  Google Scholar 

  25. Maier T, Schieber A, Kammerer DR, Carle R. Residues of grape (Vitis vinifera L.) seed oil production as a valuable source of phenolic antioxidants. Food Chem. 112: 551–559 (2009)

    Article  CAS  Google Scholar 

  26. Hakimuddin F, Paliyath G, Meckling K. Treatment of MCF7 breast cancer cells with a red grape wine polyphenol fraction results in disruption of calcium homeostasis and cell cycle arrest causing selective cytotoxicity. J. Agr. Food Chem. 54: 7912–7923 (2006)

    Article  CAS  Google Scholar 

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  1. Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea

    Jeehye Sung & Junsoo Lee

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  1. Jeehye Sung
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  2. Junsoo Lee
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Correspondence to Junsoo Lee.

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Sung, J., Lee, J. Antioxidant and antiproliferative activities of grape seeds from different cultivars. Food Sci Biotechnol 19, 321–326 (2010). https://doi.org/10.1007/s10068-010-0046-6

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  • DOI: https://doi.org/10.1007/s10068-010-0046-6

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