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Inverse correlation of programmed death 1 (PD-1) expression in T cells to the spinal radiologic changes in Taiwanese patients with ankylosing spondylitis

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Abstract

Programmed death 1 (PD-1) has been shown to be involved in the negative regulation of the immune response. However, the role of PD-1 in ankylosing spondylitis (AS) has not been studied. Therefore, we analyzed the expression of PD-1 in peripheral blood mononuclear cells (PBMC) from patients with AS. Twenty-three AS patients, 20 rheumatoid arthritis (RA) patients, and 25 normal healthy subjects were recruited. The percentage of the PBMC and PD-1 levels in these subjects were measured by flow cytometry. A higher percentage of CD4+ T cells was noted in AS patients than in healthy controls (37.53 ± 1.65% vs. 31.55 ± 0.92%, P < 0.01), but a similar result was not observed with regard to CD3+ T lymphocytes, CD4 + CD25 high + regulatory T cells, CD19+ B cells, and CD14+ and CD16+ monocytes/macrophages. PD-1 levels in PBMC were not significantly higher in AS patients than in RA patients or age- and gender-matched healthy controls and were also not correlated to the erythrocyte sedimentation rate, C-reactive protein, limitation of back flexion, and chest expansion in patients with AS. Of interest, the percentages of PD-1 + CD3+ T cells and PD-1 + CD4+ T cells were significantly lower in AS patients with higher modified Stokes Ankylosing Spondylitis Spinal Scores (mSASSS ≥30) than in those with lower mSASSS ( < 30; 0.07 ± 0.04% vs. 0.42 ± 0.14%, P < 0.05; 0.06 ± 0.03% vs. 0.40 ± 0.14%, P < 0.05, respectively). These results have suggested that the increased number of T helper cells lacking PD-1 may contribute to the spinal radiologic changes in AS patients.

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Authors and Affiliations

  1. Division of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, 201, Section 2, Shipai Road, Taipei, Taiwan, 11217

    Ming-Han Chen, Wei-Sheng Chen, Hui-Ting Lee, Chang-Youh Tsai & Chung-Tei Chou

  2. Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan

    Ming-Han Chen, Wei-Sheng Chen, Hui-Ting Lee, Chang-Youh Tsai & Chung-Tei Chou

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  1. Ming-Han Chen
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  2. Wei-Sheng Chen
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  3. Hui-Ting Lee
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  4. Chang-Youh Tsai
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Correspondence to Chang-Youh Tsai or Chung-Tei Chou.

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Chen, MH., Chen, WS., Lee, HT. et al. Inverse correlation of programmed death 1 (PD-1) expression in T cells to the spinal radiologic changes in Taiwanese patients with ankylosing spondylitis. Clin Rheumatol 30, 1181–1187 (2011). https://doi.org/10.1007/s10067-011-1721-6

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  • DOI: https://doi.org/10.1007/s10067-011-1721-6

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