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Association of the −1082 G/A promoter polymorphism of interleukin-10 gene with the autoantibodies production in patients with rheumatoid arthritis

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Abstract

Interleukin-10 (IL-10) is an immunoregulatory cytokine, usually considered to mediate the downregulation of the inflammatory response in rheumatoid arthritis (RA). Some effects of IL-10 are not anti-inflammatory; for example, the activation of B cells to promote autoantibody production. Allelic polymorphisms located in the promoter region of the IL-10 gene may contribute to the regulation of autoantibodies production. To examine the putative association between the −1082 G/A polymorphism in the promoter region of the IL-10 gene and the susceptibility to disease onset and severity of RA, a total of 144 patients with RA diagnosed according to the revised criteria of the American College of Rheumatology for RA were consecutively recruited into the study. Radiographic progression of RA was scored according to the Sharp/van der Heijde method. Serum levels of rheumatoid factors (RFs) were measured by enzyme-linked immunosorbent assay. Polymerase chain reaction amplification was used for the analysis of the promoter polymorphism of the IL-10 gene. We observed significant differences in genotype distribution of the −1082 G/A polymorphism between IgM RF, IgA RF, and IgG RF positive/negative subgroups of RA patients, with higher prevalence of the GG genotype within IgM RF (P g = 0.006), IgA RF (P g = 0.05), and IgG RF (P g = 0.007) negative RA patients. Results obtained in this study provide the evidence of an association between the −1082 G/A polymorphism in the IL-10 gene promoter and the production of RFs in RA patients.

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Acknowledgement

The study was supported by research project no. NR7812-3/2004, Ministry of Health of the Czech Republic.

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Authors and Affiliations

  1. Rheumatology Division, 2nd Department of Internal Medicine, St. Anne’s University Hospital Brno, Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic

    Petr Nemec & Miroslav Soucek

  2. Institute of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic

    Monika Pavkova-Goldbergova & Anna Vasku

  3. Institute of Rheumatology, Na Slupi 4, 128 50, Prague, Czech Republic

    Jindra Gatterova

  4. Rheumatology Division, Department of Internal Medicine and Hematooncology, University Hospital Brno, Faculty of Medicine, Masaryk University, Jihlavská 20, 625 00, Brno, Czech Republic

    Zdenek Fojtik

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  1. Petr Nemec
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  2. Monika Pavkova-Goldbergova
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  4. Zdenek Fojtik
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  6. Miroslav Soucek
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Correspondence to Petr Nemec.

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Nemec, P., Pavkova-Goldbergova, M., Gatterova, J. et al. Association of the −1082 G/A promoter polymorphism of interleukin-10 gene with the autoantibodies production in patients with rheumatoid arthritis. Clin Rheumatol 28, 899–905 (2009). https://doi.org/10.1007/s10067-009-1168-1

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  • DOI: https://doi.org/10.1007/s10067-009-1168-1

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