Abstract
The objective of this study is to examine the differential expression of mast cell tryptase and its receptor, protease-activated receptor-2 (PAR-2), in the synovium and synovial fluid of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Biochemical and immunohistochemical analyses were performed to determine whether the trypsin-like protease in the synovium is identical to mast cell tryptase. The effects of mast cell tryptase on the proliferation of synovial fibroblast-like cells (SFCs) and the release of IL-8 thereof were evaluated by the [3H]-thymidine incorporation and ELISA, respectively. The trypsin-like protease in the synovium of RA patients was identical to human mast cell tryptase, which was composed of two subunits: 33 and 34 kDa. The 33- and 34-kDa proteins are different glycosylated forms of the 31-kDa protein, which was unglycosylated. Mast cell tryptase activity in RA synovial fluid was significantly higher than that in OA synovial fluid, while their activities and expression in the synovium were similar. Expression of PAR-2 mRNA in the synovium was higher in RA than in OA. Mast cell tryptase containing the unglycosylated 31-kDa subunit was the predominant form in synovial fluid. RA patients had higher amounts of this subunit in their synovial fluid than OA patients. Mast cell tryptase and PAR-2 activating peptide stimulated the proliferation of SFCs and release of IL-8 from these cells. Mast cell tryptase secretion into RA synovial fluid is higher than OA synovial fluid. Mast cell tryptase in synovial fluid stimulates the proliferation of SFCs and the release of pro-inflammatory cytokines via PAR-2, which may contribute to exacerbation of synovitis in RA.
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Malone DG, Wilder RL, Saavedra-Delgado AM, Metcalfe DD (1987) Mast cell numbers in rheumatoid synovial tissues. Correlations with quantitative measures of lymphocytic infiltration and modulation by antiinflammatory therapy. Arthritis Rheum 30:130–137
Gruber BL, Schwartz LB, Ramamurthy NS, Irani AM, Marchese MJ (1988) Activation of latent rheumatoid synovial collagenase by human mast cell tryptase. J Immunol 140:3936–3942
Bridges AJ, Malone DG, Jicinsky J, Chen M, Ory P, Engber W et al (1991) Human synovial mast cell involvement in rheumatoid arthritis and osteoarthritis. Relationship to disease type, clinical activity, and antirheumatic therapy. Arthritis Rheum 34:1116–1124
Dean G, Hoyland JA, Denton J, Donn RP, Freemont AJ (1993) Mast cells in the synovium and synovial fluid in osteoarthritis. Br J Rheumatol 32:671–675
Tetlow LC, Woolley DE (1995) Distribution, activation and tryptase/chymase phenotype of mast cells in the rheumatoid lesion. Ann Rheum Dis 54:549–555
de Paulis A, Marino I, Ciccarelli A, de Crescenzo G, Concardi M, Verga L et al (1996) Human synovial mast cells. I. Ultrastructural in situ and in vitro immunologic characterization. Arthritis Rheum 39:1222–1233
Buckley MG, Gallagher PJ, Walls AF (1998) Mast cell subpopulations in the synovial tissue of patients with osteoarthritis: selective increase in numbers of tryptase-positive, chymase-negative mast cells. J Pathol 186:67–74
Gotis-Graham I, Smith MD, Parker A, McNeil HP (1998) Synovial mast cell responses during clinical improvement in early rheumatoid arthritis. Ann Rheum Dis 57:664–671
Woolley DE, Tetlow LC (2000) Mast cell activation and its relation to proinflammatory cytokine production in the rheumatoid lesion. Arthritis Res 2:65–74
Schwartz LB (1994) Tryptase: a mast cell serine protease. Methods Enzymol 244:88–100
Smith TJ, Hougland MW, Johnson DA (1984) Human lung tryptase. Purification and characterization. J Biol Chem 259:11046–11051
Asokananthan N, Graham PT, Fink J, Knight DA, Bakker AJ, McWilliam AS et al (2002) Activation of protease-activated receptor (PAR)-1, PAR-2, and PAR-4 stimulates IL-6, IL-8, and prostaglandin E2 release from human respiratory epithelial cells. J Immunol 168:3577–3585
D’Andrea MR, Rogahn CJ, Andrade-Gordon P (2000) Localization of protease-activated receptors-1 and -2 in human mast cells: indications for an amplified mast cell degranulation cascade. Biotech Histochem 75:85–90
Smith R, Ransjo M, Tatarczuch L, Song SJ, Pagel C, Morrison JR et al (2004) Activation of protease-activated receptor-2 leads to inhibition of osteoclast differentiation. J Bone Miner Res 19:507–516
Steinhoff M, Neisius U, Ikoma A, Fartasch M, Heyer G, Skov PS et al (2003) Proteinase-activated receptor-2 mediates itch: a novel pathway for pruritus in human skin. J Neurosci 23:6176–6180
Uehara A, Muramoto K, Takada H, Sugawara S (2003) Neutrophil serine proteinases activate human nonepithelial cells to produce inflammatory cytokines through protease-activated receptor 2. J Immunol 170:5690–5696
Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS et al (1988) The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 31:315–324
Altman R, Asch E, Bloch D, Bole G, Borenstein D, Brandt K et al (1986) Development of criteria for the classification and reporting of osteoarthritis. Classification of osteoarthritis of the knee. Diagnostic and therapeutic criteria committee of the American rheumatism association. Arthritis Rheum 29:1039–1049
Nakano S, Ikata T, Kinoshita I, Kanematsu J, Yasuoka S (1999) Characteristics of the protease activity in synovial fluid from patients with rheumatoid arthritis and osteoarthritis. Clin Exp Rheumatol 17:161–170
Yasuoka S, Ohnishi T, Kawano S, Tsuchihashi S, Ogawara M, Masuda K et al (1997) Purification, characterization, and localization of a novel trypsin-like protease found in the human airway. Am J Respir Cell Mol Biol 16:300–308
Suzue N, Nikawa T, Onishi Y, Yamada C, Hirasaka K, Ogawa T et al (2006) Ubiquitin ligase Cbl-b downregulates bone formation through suppression of IGF-I signaling in osteoblasts during denervation. J Bone Miner Res 21:722–734
Nikawa T, Towatari T, Katunuma N (1992) Purification and characterization of cathepsin J from rat liver. Eur J Biochem 204:381–393
Mishiro T, Nakano S, Takahara S, Miki M, Nakamura Y, Yasuoka S et al (2004) Relationship between cathepsin B and thrombin in rheumatoid arthritis. J Rheumatol 31:1265–1273
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Cromlish JA, Seidah NG, Marcinkiewicz M, Hamelin J, Johnson DA, Chretien M (1987) Human pituitary tryptase: molecular forms, NH2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates. J Biol Chem 262:1363–1373
Bradding P, Okayama Y, Howarth PH, Church MK, Holgate ST (1995) Heterogeneity of human mast cells based on cytokine content. J Immunol 155:297–307
Little SS, Johnson DA (1995) Human mast cell tryptase isoforms: separation and examination of substrate-specificity differences. Biochem J 307 (Pt 2):341–346
Schwartz LB, Sakai K, Bradford TR, Ren S, Zweiman B, Worobec AS et al (1995) The alpha form of human tryptase is the predominant type present in blood at baseline in normal subjects and is elevated in those with systemic mastocytosis. J Clin Invest 96:2702–2710
Buckley MG, Walters C, Wong WM, Cawley MI, Ren S, Schwartz LB et al (1997) Mast cell activation in arthritis: detection of alpha- and beta-tryptase, histamine and eosinophil cationic protein in synovial fluid. Clin Sci (Lond) 93:363–370
de Paulis A, Ciccarelli A, Marino I, de Crescenzo G, Marino D, Marone G (1997) Human synovial mast cells. II. Heterogeneity of the pharmacologic effects of antiinflammatory and immunosuppressive drugs. Arthritis Rheum 40:469–478
Chen Y, Shiota M, Ohuchi M, Towatari T, Tashiro J, Murakami M et al (2000) Mast cell tryptase from pig lungs triggers infection by pneumotropic Sendai and influenza A viruses. Purification and characterization. Eur J Biochem 267:3189–3197
Peng Q, McEuen AR, Benyon RC, Walls AF (2003) The heterogeneity of mast cell tryptase from human lung and skin. Eur J Biochem 270:270–283
Kiener HP, Baghestanian M, Dominkus M, Walchshofer S, Ghannadan M, Willheim M et al (1998) Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis. Arthritis Rheum 41:233–245
Orlean P (1992) Enzymes that recognize dolichols participate in three glycosylation pathways and are required for protein secretion. Biochem Cell Biol 70(6):438–447
Kukuruzinska MA, Lennon K (1998) Protein N-glycosylation: molecular genetics and functional significance. Crit Rev Oral Biol Med 9:415–448
Brodsky JL (2005) An in vitro assay for the selective endoplasmic reticulum associated degradation of an unglycosylated secreted protein. Methods 35:354–359
Sareneva T, Pirhonen J, Cantell K, Kalkkinen N, Julkunen I (1994) Role of N-glycosylation in the synthesis, dimerization and secretion of human interferon-gamma. Biochem J 303:831–840
Hirooka T, Suganuma N, Furuhashi M, Kitagawa T, Ando H, Kikkawa F et al (1996) Synthesis and release of glycosylated prolactin in transfected cells with human prolactin complementary deoxyribonucleic acid. Endocr J 43:423–428
Ferrell WR, Lockhart JC, Kelso EB, Dunning L, Plevin R, Meek SE et al (2003) Essential role for proteinase-activated receptor-2 in arthritis. J Clin Invest 111:35–41
Matsushima R, Takahashi A, Nakaya Y, Maezawa H, Miki M, Nakamura Y et al (2006) Human airway trypsin-like protease stimulates human bronchial fibroblast proliferation in a protease-activated receptor-2-dependent pathway. Am J Physiol Lung Cell Mol Physiol 290:L385–L395
Dery O, Corvera CU, Steinhoff M, Bunnett NW (1998) Proteinase-activated receptors: novel mechanisms of signaling by serine proteases. Am J Physiol 274:C1429–C1452
Hirano F, Kobayashi A, Hirano Y, Nomura Y, Fukawa E, Makino I (2002) Thrombin-induced expression of RANTES mRNA through protease activated receptor-1 in human synovial fibroblasts. Ann Rheum Dis 61:834–837
Yang YH, Hall P, Little CB, Fosang AJ, Milenkovski G, Santos L et al (2005) Reduction of arthritis severity in protease-activated receptor-deficient mice. Arthritis Rheum 52:1325–1332
Acknowledgments
We gratefully acknowledged Yukio Higuchi M.D. and Yoshiteru Kawasaki M.D. (Tokushima Prefectural Central Hospital) for collecting the control specimens, and Yuichi Ikeda M.D. and Sachiko Kawano Ph.D. for excellent technical assistance. Financial support for this study was provided by a Grant-in-Aid for Scientific Research (C).
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Nakano, S., Mishiro, T., Takahara, S. et al. Distinct expression of mast cell tryptase and protease activated receptor-2 in synovia of rheumatoid arthritis and osteoarthritis. Clin Rheumatol 26, 1284–1292 (2007). https://doi.org/10.1007/s10067-006-0495-8
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DOI: https://doi.org/10.1007/s10067-006-0495-8