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Allelic frequency of the MCP-1 promoter −2518 polymorphism in the Turkish population and in Turkish patients with juvenile rheumatoid arthritis

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Abstract

Although genetic and environmental factors contribute to the pathogenesis of juvenile rheumathoid arthritis (JRA), the etiology and pathogenesis remain controversial. The objective of this study was to investigate genotypic and allelic frequencies of monocyte chemoattractant protein-1 (MCP-1) gene −2518 (G/A) polymorphism in the healthy Turkish population and patients with JRA. Genomic DNA was collected from 66 JRA patients and 150 healthy individuals. To evaluate the association of the −2518 (G/A) MCP-1 gene polymorphism with the outcome of JRA, we analyzed the types of JRA and the score on the childhood health assessment questionnaire (C-HAQ score). In the healthy Turkish population, the frequencies of A and G alleles were 71 and 29%, respectively. No significant difference was observed between the JRA patients and healthy subjects in the distribution allelic and genotypic frequencies of the −2518 (G/A) MCP-1 gene polymorphism (p>0.05). However, the AG genotype was found to be higher and the AA genotype was found to be lower in the patients with systemic type JRA compared to those with the other types of JRA (p=0.019). When the JRA patients were evaluated according to the C-HAQ score, we found that the −2518 (G/A) MCP-1 gene polymorphism did not relate the prognosis (p>0.05). AG genotype was found to be higher in the systemic type of JRA. The results indicate that MCP-1 gene polymorphism might slightly associate with patients with systemic JRA. Further studies are needed to elucidate the role of this polymorphism in the pathogenesis of JRA in various populations because this polymorphism has a functional significance and an ethnic difference.

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Authors and Affiliations

  1. Department of Pediatrics, Ege University Faculty of Medicine, Izmir, Turkey

    A. Ruhi Özyürek, Zülal Ülger & Ertürk Levent

  2. Department of Pediatrics, Pamukkale University Faculty of Medicine, Denizli, Turkey

    Dolunay Gürses

  3. Department of Pediatrics, SSK Tepecik Hospital, Izmir, Turkey

    A. Rahmi Bakiler

  4. Department of Pediatrics—Laboratory of Molecular Medicine, Ege University Faculty of Medicine, Izmir, Turkey

    Afig Berdeli

  5. Department of Pediatric Cardiology, Ege University Faculty of Medicine, 35100, Bornova, İzmir, Turkey

    Dolunay Gürses

Authors
  1. A. Ruhi Özyürek
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  2. Dolunay Gürses
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  3. Zülal Ülger
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  4. Ertürk Levent
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  5. A. Rahmi Bakiler
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  6. Afig Berdeli
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Correspondence to Dolunay Gürses.

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Özyürek, A.R., Gürses, D., Ülger, Z. et al. Allelic frequency of the MCP-1 promoter −2518 polymorphism in the Turkish population and in Turkish patients with juvenile rheumatoid arthritis. Clin Rheumatol 26, 546–550 (2007). https://doi.org/10.1007/s10067-006-0347-6

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  • DOI: https://doi.org/10.1007/s10067-006-0347-6

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