Skip to main content

Advertisement

SpringerLink
Log in

The association of −627 interleukin-10 promoter polymorphism in Chinese patients with systemic lupus erythematosus

  • Review
  • Published:
Clinical Rheumatology Aims and scope Submit manuscript

Abstract

The objective of the study is to examine whether interleukin-10 (IL-10) promoter polymorphism is a marker of susceptibility of systemic lupus erythematosus (SLE) in Chinese patients in Taiwan. The study included 119 Chinese patients with SLE. One hundred unrelated healthy individuals living in central Taiwan served as control subjects. Each polymorphism was detected as a result of a polymerase chain reaction (PCR)-based restriction analysis. The PCR product length was determined to be 412 bp (CC) whereas two fragments of 236 and 176 bp were determined to be excisable lengths (AA). The relationship between the IL-10 gene polymorphism and clinical manifestations of SLE was evaluated. For the genotype and allelic frequency, there were statistically significant differences between the SLE patients and the normal control subjects (p=0.007 and 0.003, respectively). But we did not detect any association of carriage rate of the IL-10 polymorphism and the normal control subjects (p=0.077). Furthermore, we did not detect any association of IL-10 genotype with antinuclear antibody, malar rash, photosensitivity, discoid lupus, mucosal ulcer, arthritis, serositis, hematology, immunology, involvement of central nervous system, and renal disease involvement in the SLE patients. The significant relation of −627 IL-10 genotype and allelic frequency with SLE implies that the IL-10 gene polymorphism can serve as a candidate gene marker for further study in patients with SLE in Taiwan.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Llorente L, Zou W, Levy Y, Richaud-Patin Y, Wijdenes J, Alcocer-Varela J, Morel-Fourrier B, Brouet JC, Alarcon-Segovia D, Galanaud P et al (1995) Role of interleukin 10 in the B lymphocyte hyperactivity and autoantibody production of human systemic lupus erythematosus. J Exp Med 181:839

    Article  PubMed  CAS  Google Scholar 

  2. Mongan AE, Ramdahin S, Warrington RJ (1999) Interleukin-10 response abnormalities in systemic lupus erythematosus. Scand J Immunol 46:406

    Article  Google Scholar 

  3. Lacki JK, Samborski W, Mackiewicz SH (1997) Interleukin-10 and interleukin-6 in lupus erythematosus and rheumatoid arthritis, correlations with acute phase proteins. Clin Rheumatol 16:275

    Article  PubMed  CAS  Google Scholar 

  4. Llorente L, Richaud-Patin Y, Fior R, Alcocer-Varela J, Wijdenes J, Fourrier BM, Galanaud P, Emilie D (1994) In vivo production of interleukin-10 by non-T cells in rheumatoid arthritis, Sjogren’s syndrome, and systemic lupus erythematosus: a potential mechanism of B lymphocyte hyperactivity and autoimmunity. Arthritis Rheum 37:1647

    PubMed  CAS  Google Scholar 

  5. Howard M, O’Garra A, Ishida H, de Waal Malefyt R, de Vries J (1992) Biological properties of interleukin 10. J Clin Immunol 12:239–247

    Article  PubMed  CAS  Google Scholar 

  6. Chernoff AE, Granowitz EV, Shapiro L, Vannier E, Lonnemann G, Angel JB, Kennedy JS, Rabson AR, Wolff SM, Dinarello CA (1995) A randomized, controlled trial of IL-10 in humans. Inhibition of inflammatory cytokine production and immune responses. J Immunol 154:5492–5499

    PubMed  CAS  Google Scholar 

  7. Te Velde AA, de Waal Malefijt R, Huijbens RJ, de Vries JE, Figdor CG (1992) IL-10 stimulates monocyte FcγR surface expression and cytotoxic activity: distinct regulation of antibody-dependent cellular cytotoxicity by IFN-γ, IL-4, and IL-10. J Immunol 149:4048

    Google Scholar 

  8. Lalani I, Bhol K, Ahmed AR (1997) Interleukin-10: biology, role in inflammation and autoimmunity. Ann Allergy Asthma Immunol 79:469

    Article  PubMed  CAS  Google Scholar 

  9. Llorente L, Zou W, Levy Y, Richaud-Patin Y, Wijdenes J, Alcocer-Varela J, Morel-Fourrier B, Brouet JC, Alarcon-Segovia D, Galanaud P et al (1995) Role of interleukin 10 in the B lymphocyte hyperactivity and autoantibody production of human systemic lupus erythematosus. J Exp Med 181:839

    Article  PubMed  CAS  Google Scholar 

  10. Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25:1271

    PubMed  CAS  Google Scholar 

  11. Eskdale J, Kube D, Tesch H, Gallagher G (1997) Mapping of the human IL10 gene and further characterization of the 5′ flanking sequence. Immunogenetics 46(2):120–128

    Article  PubMed  CAS  Google Scholar 

  12. Bienvenu J, Doche C, Gutowski MC, Lenoble M, Lepape A, Perdrix JP (1995) Production of pro-inflammatory cytokines and cytokines involved in the TH1/TH2 balance is modulated by pentoxifylline. J Cardiovasc Pharmacol 25(Suppl 2):80–84

    Article  Google Scholar 

  13. Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV (1997) An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 24(1):1–8 (Feb)

    PubMed  CAS  Google Scholar 

  14. Lazarus M, Hajeer AH, Turner D, Sinnott P, Worthington J, Ollier WE, Hutchinson IV (1997) Genetic variation in the interleukin 10 gene promoter and systemic lupus erythematosus. J Rheumatol 24:2314–2317

    PubMed  CAS  Google Scholar 

  15. Mok CC, Lanchbury JS, Chan DW, Lau CS (1998) Interleukin-10 promoter polymorphisms in Southern Chinese patients with systemic lupus erythematosus. Arthritis Rheum 41:1090–1095

    Article  PubMed  CAS  Google Scholar 

  16. Gibson AW, Edberg JC, Wu J, Westendom RG, Huizinga TW, Kimberly RP (2001) Novel single nucleotide polymorphism in the distal IL-10 promoter affect IL-10 production and enhance the risk of systemic lupus erythematosus. J Immunol 166:3915–3922

    PubMed  CAS  Google Scholar 

  17. Lim S, Crawley E, Woo P, Barnes PJ (1998) Haplotype associated with low interleukin-10 production in patients with severe asthma. Lancet 352(9122):113 (Jul 11)

    Article  PubMed  CAS  Google Scholar 

  18. Borish L, Aarons A, Rumbyrt J, Cvietusa P, Negri J, Wenzel S (1996) Interleukin-10 regulation in normal subjects and patients with asthma. J Allergy Clin Immunol 97(6):1288–1296 (Jun)

    Article  PubMed  CAS  Google Scholar 

  19. Grove J, Daly AK, Bassendine MF, Gilvarry E, Day CP (2000) Interleukin 10 promoter region polymorphisms and susceptibility to advanced alcoholic liver disease. Gut 46(4):540–545 (Apr)

    Article  PubMed  CAS  Google Scholar 

  20. Guseva IA, Omarbekova Zh, Myakotkin VA (2003) Polymorphism of FcgRIIIA-158F/V and promotion region of IL-10 genes in systemic lupus erythematosus in Kazakhs. Therapeutic Archives 5:36

    Google Scholar 

  21. Eskdale J, Gallagher G, Verweij CL, Keijsers V, Westendorp RG, Huizinga TW (1998) Interleukin 10 secretion in relation to human IL-10 locus haplotypes. Proc Natl Acad Sci USA 5:9465

    Article  Google Scholar 

  22. Mehrian R Jr, Quismorio FP, Strassmann G, Stimmler MM, Horwitz DA, Kitridou RC, Gauderman WJ, Morrison J, Brautbar C, Jacob CO (1998) Synergistic effect between IL-10 and bcl-2 genotypes in determining susceptibility to systemic lupus erythematosus. Arthritis Rheum 41:596

    Article  PubMed  CAS  Google Scholar 

  23. Eskdale J, McNicholl J, Wordsworth P, Jonas B, Huizinga T, Field M, Gallagher G (1998) Interleukin-10 microsatellite polymorphisms and IL-10 locus alleles in rheumatoid arthritis susceptibility. Lancet 352:1282

    Article  PubMed  CAS  Google Scholar 

  24. Keijsers V, Verweij CL, Westendorp RGJ, Breedveld FC, Huizinga TWJ (1997) IL-10 polymorphisms in relation to production and rheumatoid arthritis. Arthritis Rheum 40(Suppl 9):S179

    Google Scholar 

  25. Lazarus M, Hajeer AH, Turner D, Sinnott P, Worthington J, Ollier WE, Hutchinson IV (1997) Genetic variation in the interleukin 10 gene promoter and systemic lupus erythematosus. J Rheumatol 24:2314

    PubMed  CAS  Google Scholar 

  26. Mok CC, Lanchbury JS, Chan DW, Lau CS (1998) Interleukin-10 promoter polymorphisms in Southern Chinese patients with systemic lupus erythematosus. Arthritis Rheum 41:1090

    Article  PubMed  CAS  Google Scholar 

  27. Eskdale J, Wordsworth P, Bowman S, Field M, Gallagher G (1997) Association between polymorphisms at the human IL-10 locus and systemic lupus erythematosus. Tissue Antigens 49:635

    Article  PubMed  CAS  Google Scholar 

  28. Crawley E, Kay R, Sillibourne J, Patel P, Hutchinson I, Woo P (1999) Polymorphic haplotypes of the interleukin-10 5′ flanking region determine variable interleukin-10 transcription and are associated with particular phenotypes of juvenile rheumatoid arthritis. Arthritis Rheum 42:1101

    Article  PubMed  CAS  Google Scholar 

  29. D’Alfonso S, Rampi M, Bocchio D, Colombo G, Scorza-Smeraldi R, Momigliano-Richardi P (2000) Systemic lupus erythematosus candidate genes in the Italian population: evidence for a significant association with interleukin-10. Arthritis Rheum 43:120

    Article  PubMed  CAS  Google Scholar 

  30. Pratt RE, Dzau VJ (1999) Genomics and hypertension concepts, potentials, and opportunities. Hypertension 33:238–247

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Nephrology, China Medical University Hospital, Taichung, Taiwan, Republic of China

    Po-Wen Lin & Chiu-Ching Huang

  2. Division of Immunology and Rheumatology, China Medical University Hospital, Taichung, Taiwan, Republic of China

    Chung-Ming Huang

  3. Taichung Healthcare and Management University, China Medical University Hospital, Taichung, Taiwan, Republic of China

    Chang-Hai Tsai & Jeffrey J. P. Tsai

  4. Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, Republic of China

    Chin-Ping Chang & Fuu-Jen Tsai

  5. Department of Medicine Genetics, China Medical University Hospital, No. 2 Yuh Der Road, Taichung, Taiwan, Republic of China

    Chin-Ping Chang & Fuu-Jen Tsai

  6. College of Chinese Medicine, China Medical University Hospital, Taichung, Taiwan, Republic of China

    Fuu-Jen Tsai

Authors
  1. Po-Wen Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chung-Ming Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chiu-Ching Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chang-Hai Tsai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jeffrey J. P. Tsai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chin-Ping Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fuu-Jen Tsai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fuu-Jen Tsai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, PW., Huang, CM., Huang, CC. et al. The association of −627 interleukin-10 promoter polymorphism in Chinese patients with systemic lupus erythematosus. Clin Rheumatol 26, 298–301 (2007). https://doi.org/10.1007/s10067-006-0329-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10067-006-0329-8

Keywords

Search

Navigation