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Diagnostic value of antiagalactosyl IgG antibodies in rheumatoid arthritis

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Abstract

Our objective in this study was to explore the diagnostic value of antiagalactosyl IgG antibodies in rheumatoid arthritis (RA). The study comprised 266 Japanese patients with systemic autoimmune diseases, including 60 with RA. Human agalactosyl IgG was prepared enzymatically, and the serum levels of antiagalactosyl IgG antibodies were determined using a lectin enzyme immunoassay. Serum IgG and IgM rheumatoid factors (RF) were measured using laser nephelometry for IgM (LN-RF) and an enzyme-linked immunosorbent assay for IgG (IgG-RF). Antiagalactosyl IgG antibodies were significantly more common in patients with RA than in those without (78% vs. 18%, odds ratio (OR) 16.51, 95% confidence interval (CI) 8.12–33.58, p<0.0001). Patients with RA also had a higher frequency of LN-RF than those without RA (75% vs. 28%, OR 7.81, 95% CI 3.91–15.58, p< 0.001). The specificity of antiagalactosyl IgG antibodies for RA was significantly higher than that of LN-RF (82% vs. 72%, p<0.0011). There was a significant correlation between titers of antiagalactosyl IgG antibodies and C-reactive protein levels. Antiagalactosyl IgG antibodies are more specific markers for RA than conventional LN-RF, and may provide useful information for the diagnosis of RA.

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Abbreviations

AKA:

Antikeratin antibodies

RA:

Rheumatoid arthritis

RF:

Rheumatoid factor

SLE:

Systemic lupus erythematosus

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Acknowledgements

Supported in part by grants from the Ministry of Health and Welfare of Japan, and from the Ministry of Education, Science, Technology and Culture of Japan.

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Authors and Affiliations

  1. Department of Medicine II, Hokkaido University Graduate School of Medicine, N15 W7, Kitaku, 060–8638, Sapporo, Japan

    Hemlata Das, Tatsuya Atsumi, Yoshiharu Amasaki, Kenji Ichikawa, Olga Amengual & Takao Koike

  2. Department of Laboratory Medicine, Hokkaido University Graduate School of Medicine, N15 W7, Kitaku, 060–8638, Sapporo, Japan

    Yukari Fukushima, Hitoshi Shibuya & Keiko Ito

  3. Research and Development of Clinical Diagnostics Division, Tsukuba Research Laboratory, Eisai Co. Ltd, Tsukuba, Ibaraki, Japan

    Yuji Yamada

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  1. Hemlata Das
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  2. Tatsuya Atsumi
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  3. Yukari Fukushima
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  4. Hitoshi Shibuya
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  5. Keiko Ito
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  6. Yuji Yamada
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  7. Yoshiharu Amasaki
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  8. Kenji Ichikawa
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  9. Olga Amengual
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  10. Takao Koike
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Correspondence to Tatsuya Atsumi.

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Das, H., Atsumi, T., Fukushima, Y. et al. Diagnostic value of antiagalactosyl IgG antibodies in rheumatoid arthritis. Clin Rheumatol 23, 218–222 (2004). https://doi.org/10.1007/s10067-003-0860-9

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  • DOI: https://doi.org/10.1007/s10067-003-0860-9

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